TEFM_MOUSE
ID TEFM_MOUSE Reviewed; 364 AA.
AC Q5SSK3;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Transcription elongation factor, mitochondrial;
DE Flags: Precursor;
GN Name=Tefm;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Stomach;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transcription elongation factor which increases mitochondrial
CC RNA polymerase processivity. Regulates transcription of the
CC mitochondrial genome, including genes important for the oxidative
CC phosphorylation machinery (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with POLRMT. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}. Mitochondrion
CC matrix, mitochondrion nucleoid {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TEFM family. {ECO:0000305}.
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DR EMBL; AK146639; BAE27323.1; -; mRNA.
DR EMBL; AL663057; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS36241.1; -.
DR RefSeq; NP_899098.2; NM_183275.2.
DR AlphaFoldDB; Q5SSK3; -.
DR SMR; Q5SSK3; -.
DR BioGRID; 212921; 2.
DR STRING; 10090.ENSMUSP00000059304; -.
DR PhosphoSitePlus; Q5SSK3; -.
DR EPD; Q5SSK3; -.
DR MaxQB; Q5SSK3; -.
DR PaxDb; Q5SSK3; -.
DR PeptideAtlas; Q5SSK3; -.
DR PRIDE; Q5SSK3; -.
DR ProteomicsDB; 263161; -.
DR Antibodypedia; 7311; 105 antibodies from 19 providers.
DR DNASU; 68550; -.
DR Ensembl; ENSMUST00000050207; ENSMUSP00000059304; ENSMUSG00000046909.
DR GeneID; 68550; -.
DR KEGG; mmu:68550; -.
DR UCSC; uc007klj.1; mouse.
DR CTD; 79736; -.
DR MGI; MGI:1915800; Tefm.
DR VEuPathDB; HostDB:ENSMUSG00000046909; -.
DR eggNOG; ENOG502QPVB; Eukaryota.
DR GeneTree; ENSGT00390000010581; -.
DR HOGENOM; CLU_066790_0_0_1; -.
DR InParanoid; Q5SSK3; -.
DR OMA; ESPQMAQ; -.
DR OrthoDB; 1096937at2759; -.
DR PhylomeDB; Q5SSK3; -.
DR TreeFam; TF325413; -.
DR BioGRID-ORCS; 68550; 24 hits in 71 CRISPR screens.
DR ChiTaRS; Tefm; mouse.
DR PRO; PR:Q5SSK3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SSK3; protein.
DR Bgee; ENSMUSG00000046909; Expressed in intercostal muscle and 201 other tissues.
DR ExpressionAtlas; Q5SSK3; baseline and differential.
DR Genevisible; Q5SSK3; MM.
DR GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR GO; GO:0042645; C:mitochondrial nucleoid; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:1990904; C:ribonucleoprotein complex; ISS:UniProtKB.
DR GO; GO:0030337; F:DNA polymerase processivity factor activity; ISS:UniProtKB.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006390; P:mitochondrial transcription; ISS:UniProtKB.
DR GO; GO:0006119; P:oxidative phosphorylation; ISS:UniProtKB.
DR GO; GO:0006392; P:transcription elongation from mitochondrial promoter; IEA:InterPro.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR010994; RuvA_2-like.
DR InterPro; IPR039150; TEFM.
DR PANTHER; PTHR21053; PTHR21053; 1.
DR SUPFAM; SSF47781; SSF47781; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Mitochondrion nucleoid; Reference proteome; Transcription;
KW Transcription regulation; Transit peptide.
FT TRANSIT 1..39
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 40..364
FT /note="Transcription elongation factor, mitochondrial"
FT /id="PRO_0000406330"
SQ SEQUENCE 364 AA; 41874 MW; 37A54216F0E53DF4 CRC64;
MAWRTNLACL IKAGGRRWFP VPEYSSLPPV LNNTCSVRKS TAPEKRVASV AACDTDGKEP
GNPLDRLFSL EQQASILQVL NTASDKELEA FRLLRGRKSV NIVEHRKKFG PFQRLENLID
VPLIQYKTAV EVCNSILCPE NRRRKKSQEK WLLRKFIKPG VEQERLKAVK SIVSIVFGTR
RIAWAHLDRR PTVLDWQQTE CWKLTNKTYP TSFYLEEISS VISKIPKADL YILEKSGLSI
QNTSLLPILL HFLITEAMLY ALLNKTFAED GQHRVLSINR NAVGKHFDLM IGDTRTSGRE
LVKQFLSESV LKERPRVFFP QDLLVQYRQK VVKSSYRIEE LYDSLLQAVA FYELVFGKDS
ELKC
