TEF_CHICK
ID TEF_CHICK Reviewed; 319 AA.
AC Q92172; Q788X3; Q92057;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Transcription factor VBP;
DE AltName: Full=Thyrotroph embryonic factor homolog;
DE AltName: Full=Vitellogenin gene-binding protein;
GN Name=TEF; Synonyms=VBP;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [GENOMIC DNA]
RP OF 1-60, ALTERNATIVE PROMOTER USAGE, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=White leghorn; TISSUE=Embryonic fibroblast;
RX PubMed=7984425; DOI=10.1093/nar/22.22.4733;
RA Burch J.B.E., Davis D.L.;
RT "Alternative promoter usage and splicing options result in the differential
RT expression of mRNAs encoding four isoforms of chicken VBP, a member of the
RT PAR subfamily of bZIP transcription factors.";
RL Nucleic Acids Res. 22:4733-4741(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, SUBUNIT, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF LEU-278 AND LEU-285.
RC TISSUE=Liver;
RX PubMed=1922023; DOI=10.1128/mcb.11.10.4863-4875.1991;
RA Iyer S.V., Davis D.L., Seal S.N., Burch J.B.E.;
RT "Chicken vitellogenin gene-binding protein, a leucine zipper transcription
RT factor that binds to an important control element in the chicken
RT vitellogenin II promoter, is related to rat DBP.";
RL Mol. Cell. Biol. 11:4863-4875(1991).
CC -!- FUNCTION: Transcription factor that binds to and transactivates the
CC vitellogenin II (VTG2) promoter. Binds to the palindromic sequence 5'-
CC GTTTACATAAAC-3'. {ECO:0000269|PubMed:1922023,
CC ECO:0000269|PubMed:7984425}.
CC -!- SUBUNIT: Binds DNA as a homodimer or a heterodimer. Exists as a stable
CC dimer in the absence of DNA. {ECO:0000269|PubMed:1922023}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage, Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=Alpha/beta;
CC IsoId=Q92172-1; Sequence=Displayed;
CC Name=2; Synonyms=Beta/beta;
CC IsoId=Q92172-2; Sequence=VSP_051642;
CC Name=3; Synonyms=Alpha/alpha;
CC IsoId=Q92172-3; Sequence=VSP_051643;
CC Name=4; Synonyms=Beta/alpha;
CC IsoId=Q92172-4; Sequence=VSP_051642, VSP_051643;
CC -!- TISSUE SPECIFICITY: Isoform 1 and isoform 3 are expressed in a variety
CC of somatic tissues, including liver, heart, intestine, stomach and
CC kidney. Both isoforms are also expressed in hepatoma (LMH) cells and in
CC embryonic fibroblast cell lines. Isoform 2 and isoform 4 are expressed
CC in adult heart and intestine. {ECO:0000269|PubMed:1922023,
CC ECO:0000269|PubMed:7984425}.
CC -!- DEVELOPMENTAL STAGE: Isoform 3 is expressed as early as embryonic day
CC 10. {ECO:0000269|PubMed:1922023}.
CC -!- MISCELLANEOUS: [Isoform 1]: Produced by alternative promoter usage.
CC -!- MISCELLANEOUS: [Isoform 2]: Produced by alternative promoter usage.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: Produced by alternative splicing of isoform
CC 1. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 4]: Produced by alternative splicing of isoform
CC 2. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the bZIP family. PAR subfamily. {ECO:0000305}.
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DR EMBL; U09221; AAA82156.1; -; mRNA.
DR EMBL; U09222; AAA82157.1; -; Genomic_DNA.
DR EMBL; U09223; AAA82158.1; -; Genomic_DNA.
DR PIR; A41524; A41524.
DR PIR; S50109; S50109.
DR RefSeq; NP_990713.1; NM_205382.1. [Q92172-2]
DR AlphaFoldDB; Q92172; -.
DR SMR; Q92172; -.
DR STRING; 9031.ENSGALP00000037167; -.
DR PaxDb; Q92172; -.
DR Ensembl; ENSGALT00000037963; ENSGALP00000037167; ENSGALG00000011958. [Q92172-2]
DR Ensembl; ENSGALT00000064953; ENSGALP00000048592; ENSGALG00000011958. [Q92172-1]
DR Ensembl; ENSGALT00000096528; ENSGALP00000071276; ENSGALG00000011958. [Q92172-4]
DR GeneID; 396342; -.
DR KEGG; gga:396342; -.
DR CTD; 7008; -.
DR VEuPathDB; HostDB:geneid_396342; -.
DR eggNOG; KOG3119; Eukaryota.
DR GeneTree; ENSGT00940000156578; -.
DR HOGENOM; CLU_051922_2_0_1; -.
DR InParanoid; Q92172; -.
DR OMA; PDCVEVD; -.
DR OrthoDB; 1023460at2759; -.
DR PhylomeDB; Q92172; -.
DR TreeFam; TF315869; -.
DR PRO; PR:Q92172; -.
DR Proteomes; UP000000539; Chromosome 1.
DR Bgee; ENSGALG00000011958; Expressed in cerebellum and 13 other tissues.
DR ExpressionAtlas; Q92172; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR040223; PAR_bZIP.
DR InterPro; IPR029832; TEF/VBP.
DR PANTHER; PTHR11988; PTHR11988; 1.
DR PANTHER; PTHR11988:SF24; PTHR11988:SF24; 1.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative promoter usage; Alternative splicing; DNA-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..319
FT /note="Transcription factor VBP"
FT /id="PRO_0000076516"
FT DOMAIN 243..306
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 245..265
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 266..273
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 145..180
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..60
FT /note="MPGRAAHQEAAAAGGAAAEPTAAGGSAGAVAQQPEQQGLAGAFPLVLKKLME
FT NPPRDARL -> MSVCNSAGGPAALDFPEVLKSLLEYSLPWTTKMT (in isoform
FT 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:7984425"
FT /id="VSP_051642"
FT VAR_SEQ 313..319
FT /note="FDLSDSE -> L (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:1922023"
FT /id="VSP_051643"
FT MUTAGEN 278
FT /note="L->V: Complete loss of binding to VTG2 promoter in
FT isoform 3."
FT /evidence="ECO:0000269|PubMed:1922023"
FT MUTAGEN 285
FT /note="L->V: Complete loss of binding to VTG2 promoter in
FT isoform 3."
FT /evidence="ECO:0000269|PubMed:1922023"
SQ SEQUENCE 319 AA; 34954 MW; 68218FF969A51804 CRC64;
MPGRAAHQEA AAAGGAAAEP TAAGGSAGAV AQQPEQQGLA GAFPLVLKKL MENPPRDARL
DKEKKIKLEE DEAAAASTMA VSASLMPPIW DKTIPYDGES FHLEYMDLDE FLLENGIPSS
PTHLDLNQNP LMPVAKLEEK EPASASTGSP VSSSSTAVYQ QSEAASSTES PPQNERNTPS
PIDPDCVEVE VNFNPDPADL VLSSVPGGEL FNPRKHKFTE EDLKPQPMIK KAKKVFVPDE
QKDEKYWTRR KKNNVAAKRS RDARRLKENQ ITIRAAFLEK ENTALRTEVA ELRKEVGRCK
NIVSKYETRY GPFDLSDSE
