TEF_DROME
ID TEF_DROME Reviewed; 649 AA.
AC Q7K4M4; B6UYC2; B6UYC7; B6UYC8; B6UYD0; B6UYD1; B6UYD2; B6UYD3; B6UYD5;
AC B6UYD6; B6UYD8; B6UYE0; B6UYE5; B6UYE6; B6UYE9; B6UYF1; B6UYF2; B6UYF3;
AC Q5MBE0;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Protein teflon {ECO:0000303|PubMed:11139508};
GN Name=tef {ECO:0000312|EMBL:AAK93242.1, ECO:0000312|FlyBase:FBgn0086350};
GN ORFNames=CG8961;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAV90635.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF CYS-38.
RX PubMed=16816414; DOI=10.1534/genetics.106.061556;
RA Arya G.H., Lodico M.J.P., Ahmad O.I., Amin R., Tomkiel J.E.;
RT "Molecular characterization of teflon, a gene required for meiotic autosome
RT segregation in male Drosophila melanogaster.";
RL Genetics 174:125-134(2006).
RN [2] {ECO:0000312|EMBL:AAF57911.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAF57911.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAK93242.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93242.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305, ECO:0000312|EMBL:ACI96588.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 74-430, AND VARIANTS GLU-90; TYR-97;
RP ILE-122; ASN-131; SER-155; CYS-162; MET-211; SER-240; SER-292; ASP-346;
RP ASN-349 AND THR-383.
RC STRAIN=MW11 {ECO:0000312|EMBL:ACI96578.1},
RC MW25 {ECO:0000312|EMBL:ACI96583.1}, MW27 {ECO:0000312|EMBL:ACI96584.1},
RC MW38 {ECO:0000312|EMBL:ACI96600.1}, MW56 {ECO:0000312|EMBL:ACI96604.1},
RC MW6 {ECO:0000312|EMBL:ACI96605.1}, MW63 {ECO:0000312|EMBL:ACI96606.1},
RC MW9 {ECO:0000312|EMBL:ACI96610.1}, NC301 {ECO:0000312|EMBL:ACI96585.1},
RC NC303 {ECO:0000312|EMBL:ACI96586.1}, NC304 {ECO:0000312|EMBL:ACI96587.1},
RC NC306 {ECO:0000312|EMBL:ACI96588.1}, NC319 {ECO:0000312|EMBL:ACI96589.1},
RC NC322 {ECO:0000312|EMBL:ACI96590.1}, NC335 {ECO:0000312|EMBL:ACI96591.1},
RC NC336 {ECO:0000312|EMBL:ACI96592.1}, NC350 {ECO:0000312|EMBL:ACI96593.1},
RC NC357 {ECO:0000312|EMBL:ACI96594.1}, NC358 {ECO:0000312|EMBL:ACI96595.1},
RC NC359 {ECO:0000312|EMBL:ACI96596.1}, NC361 {ECO:0000312|EMBL:ACI96597.1},
RC NC362 {ECO:0000312|EMBL:ACI96598.1}, NC375 {ECO:0000312|EMBL:ACI96599.1},
RC NC390 {ECO:0000312|EMBL:ACI96601.1}, NC397 {ECO:0000312|EMBL:ACI96602.1},
RC NC399 {ECO:0000312|EMBL:ACI96603.1}, NC732 {ECO:0000312|EMBL:ACI96607.1},
RC NC740 {ECO:0000312|EMBL:ACI96608.1}, and
RC NC774 {ECO:0000312|EMBL:ACI96609.1};
RX PubMed=18984573; DOI=10.1534/genetics.108.093807;
RA Anderson J.A., Gilliland W.D., Langley C.H.;
RT "Molecular population genetics and evolution of Drosophila meiosis genes.";
RL Genetics 181:177-185(2009).
RN [6] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=11139508; DOI=10.1093/genetics/157.1.273;
RA Tomkiel J.E., Wakimoto B.T., Briscoe A. Jr.;
RT "The teflon gene is required for maintenance of autosomal homolog pairing
RT at meiosis I in male Drosophila melanogaster.";
RL Genetics 157:273-281(2001).
CC -!- FUNCTION: Specifically required in males for proper segregation of
CC autosomal bivalents at meiosis I. Expression is required in the male
CC germ line prior to spermatocyte stage S4. May have a role as a bridging
CC molecule maintaining adhesion to hold autosome bivalents together via
CC heterochromatic connections. {ECO:0000269|PubMed:11139508,
CC ECO:0000269|PubMed:16816414}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11139508,
CC ECO:0000269|PubMed:16816414}. Chromosome. Note=Male meiotic
CC chromosomes. {ECO:0000269|PubMed:11139508,
CC ECO:0000269|PubMed:16816414}.
CC -!- TISSUE SPECIFICITY: Expressed at a low level in a variety of tissues,
CC highest expression is in testis. {ECO:0000269|PubMed:11139508,
CC ECO:0000269|PubMed:16816414}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development. {ECO:0000269|PubMed:16816414}.
CC -!- MISCELLANEOUS: Drosophilid specific gene duplication generates rgr and
CC tef. Teflon has a function unique to Drosophilids.
CC {ECO:0000269|PubMed:16816414}.
CC -!- SIMILARITY: Belongs to the Teflon family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY840220; AAV90635.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF57911.1; -; Genomic_DNA.
DR EMBL; AY051818; AAK93242.1; -; mRNA.
DR EMBL; FJ219520; ACI96578.1; -; Genomic_DNA.
DR EMBL; FJ219525; ACI96583.1; -; Genomic_DNA.
DR EMBL; FJ219526; ACI96584.1; -; Genomic_DNA.
DR EMBL; FJ219527; ACI96585.1; -; Genomic_DNA.
DR EMBL; FJ219528; ACI96586.1; -; Genomic_DNA.
DR EMBL; FJ219529; ACI96587.1; -; Genomic_DNA.
DR EMBL; FJ219530; ACI96588.1; -; Genomic_DNA.
DR EMBL; FJ219531; ACI96589.1; -; Genomic_DNA.
DR EMBL; FJ219532; ACI96590.1; -; Genomic_DNA.
DR EMBL; FJ219533; ACI96591.1; -; Genomic_DNA.
DR EMBL; FJ219534; ACI96592.1; -; Genomic_DNA.
DR EMBL; FJ219535; ACI96593.1; -; Genomic_DNA.
DR EMBL; FJ219536; ACI96594.1; -; Genomic_DNA.
DR EMBL; FJ219537; ACI96595.1; -; Genomic_DNA.
DR EMBL; FJ219538; ACI96596.1; -; Genomic_DNA.
DR EMBL; FJ219539; ACI96597.1; -; Genomic_DNA.
DR EMBL; FJ219540; ACI96598.1; -; Genomic_DNA.
DR EMBL; FJ219541; ACI96599.1; -; Genomic_DNA.
DR EMBL; FJ219542; ACI96600.1; -; Genomic_DNA.
DR EMBL; FJ219543; ACI96601.1; -; Genomic_DNA.
DR EMBL; FJ219544; ACI96602.1; -; Genomic_DNA.
DR EMBL; FJ219545; ACI96603.1; -; Genomic_DNA.
DR EMBL; FJ219546; ACI96604.1; -; Genomic_DNA.
DR EMBL; FJ219547; ACI96605.1; -; Genomic_DNA.
DR EMBL; FJ219548; ACI96606.1; -; Genomic_DNA.
DR EMBL; FJ219549; ACI96607.1; -; Genomic_DNA.
DR EMBL; FJ219550; ACI96608.1; -; Genomic_DNA.
DR EMBL; FJ219551; ACI96609.1; -; Genomic_DNA.
DR EMBL; FJ219552; ACI96610.1; -; Genomic_DNA.
DR RefSeq; NP_523766.1; NM_079042.4.
DR AlphaFoldDB; Q7K4M4; -.
DR BioGRID; 62620; 3.
DR IntAct; Q7K4M4; 3.
DR STRING; 7227.FBpp0086165; -.
DR PaxDb; Q7K4M4; -.
DR DNASU; 36920; -.
DR EnsemblMetazoa; FBtr0087014; FBpp0086165; FBgn0086350.
DR GeneID; 36920; -.
DR KEGG; dme:Dmel_CG8961; -.
DR UCSC; CG8961-RA; d. melanogaster.
DR CTD; 7008; -.
DR FlyBase; FBgn0086350; tef.
DR VEuPathDB; VectorBase:FBgn0086350; -.
DR eggNOG; ENOG502T9CV; Eukaryota.
DR HOGENOM; CLU_014432_0_0_1; -.
DR InParanoid; Q7K4M4; -.
DR OMA; TKEHNVY; -.
DR OrthoDB; 602441at2759; -.
DR PhylomeDB; Q7K4M4; -.
DR BioGRID-ORCS; 36920; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36920; -.
DR PRO; PR:Q7K4M4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0086350; Expressed in cleaving embryo and 22 other tissues.
DR Genevisible; Q7K4M4; DM.
DR GO; GO:0030849; C:autosome; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007060; P:male meiosis chromosome segregation; IMP:FlyBase.
DR GO; GO:0051308; P:male meiosis chromosome separation; IMP:UniProtKB.
DR GO; GO:0007141; P:male meiosis I; IMP:FlyBase.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Chromosome; Chromosome partition; Meiosis; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..649
FT /note="Protein teflon"
FT /id="PRO_0000377408"
FT ZN_FING 33..56
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 599..621
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 625..648
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 78..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 90
FT /note="G -> E (in strain: NC732 and NC390)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 97
FT /note="D -> Y (in strain: MW6)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 122
FT /note="M -> I (in strain: MW11, MW27, NC301 and NC397)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 131
FT /note="S -> N (in strain: MW11)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 155
FT /note="N -> S (in strain: MW11)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 162
FT /note="R -> C (in strain: MW25, MW38, MW56, MW63, MW9,
FT NC304, NC357 and NC361)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 211
FT /note="I -> M (in strain: MW6, MW9, MW11, MW25, MW27, MW38,
FT MW56, MW63, NC301, NC304, NC357, NC361, NC390, NC397 and
FT NC732)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 240
FT /note="T -> S (in strain: MW11)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 292
FT /note="A -> S (in strain: NC357)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 346
FT /note="G -> D (in strain: NC397)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 349
FT /note="D -> N (in strain: NC335)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 383
FT /note="A -> T (in strain: MW6, MW27, NC301, NC304 and
FT NC397)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT MUTAGEN 38
FT /note="C->Y: In z5864; Induces 4th chromosome non-
FT disjunction."
FT /evidence="ECO:0000269|PubMed:16816414"
SQ SEQUENCE 649 AA; 73358 MW; F96518EB37D60E68 CRC64;
MSKFLDMLSG SQCVSLEKCG DVVVSTNDCM IALYCHFCRD LFTQLPEFLR HLQSNHSDVL
HFTKEHNVYS VEELLSGEQG KAHEDAQSAG HNSSSGDSSS LMNSEDSRAI EGSEDNSDNS
PMKPEQIGKQ SEINLLAEVT NILLQTNDKE LKPENGVFNR PRKKANNESS SLKICDLKSH
TIARTSRKRM SMVKNRILRV LDSDLSAKLE IKPPEPNSKL SITEPIQEDN IPGTCFDTPT
KPIPSSSQLS VRKSSLTEAN QICTKYAEKK TAPTMPKLLN CVPKPILTSQ QAHTNAEISE
INETYHLAAS QVTKTTKTFP VKITQIDILQ PVKLPKTLIT PINEEGVSDQ VENSTNNINN
AQSLPKENTK KFFKKRSELG IKAQGSPNKF FKIIKSKANP IIVKRVQTTS AKASTNKIQI
RSNDKTNCFA SEFNSTKIRK LKMENCVDLK SEDPCANTNT RLNKLATISS CEILKAVGLP
AITDNPIEDT LLPDELETIR KKADQFIKIY RKYDSIWNYR KICPPAKPDF ISQQIFALTR
EVNKTMLCNL ANSDIKGIIN QISVWHYNIY TQYIDLDTIS ENARYTLKLF SFLPVSFAYF
CKCCDDIFIL KKEYLKHLIS HQVRFQCTKC IKVFKYKGYY EKHLRNAHS
