BRAB_PSEAE
ID BRAB_PSEAE Reviewed; 437 AA.
AC P19072;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Branched-chain amino acid transport system 2 carrier protein;
DE AltName: Full=LIV-II;
GN Name=braB; OrderedLocusNames=PA1590;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=2111004; DOI=10.1007/bf00391754;
RA Hoshino T., Kose K., Uratani Y.;
RT "Cloning and nucleotide sequence of the gene braB coding for the sodium-
RT coupled branched-chain amino acid carrier in Pseudomonas aeruginosa PAO.";
RL Mol. Gen. Genet. 220:461-467(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Component of the LIV-II transport system for branched-chain
CC amino acids. BraB is specific for isoleucine, leucine and valine. The
CC LIV-II transport system is coupled to sodium and lithium ions.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the branched chain amino acid transporter
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X51634; CAA35961.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04979.1; -; Genomic_DNA.
DR PIR; S11497; S11497.
DR RefSeq; NP_250281.1; NC_002516.2.
DR RefSeq; WP_003114263.1; NZ_QZGE01000003.1.
DR AlphaFoldDB; P19072; -.
DR STRING; 287.DR97_299; -.
DR TCDB; 2.A.26.1.1; the branched chain amino acid:cation symporter (livcs) family.
DR PaxDb; P19072; -.
DR EnsemblBacteria; AAG04979; AAG04979; PA1590.
DR GeneID; 882083; -.
DR KEGG; pae:PA1590; -.
DR PATRIC; fig|208964.12.peg.1649; -.
DR PseudoCAP; PA1590; -.
DR HOGENOM; CLU_036807_0_0_6; -.
DR InParanoid; P19072; -.
DR OMA; AFAFSYH; -.
DR PhylomeDB; P19072; -.
DR BioCyc; PAER208964:G1FZ6-1620-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015190; F:L-leucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015803; P:branched-chain amino acid transport; IDA:PseudoCAP.
DR GO; GO:0015818; P:isoleucine transport; IBA:GO_Central.
DR GO; GO:0015820; P:leucine transport; IBA:GO_Central.
DR GO; GO:0015829; P:valine transport; IBA:GO_Central.
DR InterPro; IPR004685; Brnchd-chn_aa_trnsp_Livcs.
DR PANTHER; PTHR30588; PTHR30588; 1.
DR Pfam; PF05525; Branch_AA_trans; 1.
DR TIGRFAMs; TIGR00796; livcs; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..437
FT /note="Branched-chain amino acid transport system 2 carrier
FT protein"
FT /id="PRO_0000099764"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 369..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 437 AA; 45283 MW; 75C64F6E84985122 CRC64;
MTHLKGFDLL ALGFMTFALF LGAGNIIFPP SAGMAAGEHV WSAAFGFLLT GVGLPLLTVV
ALARVGGGIG RLTQPIGRRA GVAFAIAVYL AIGPLFATPR TAVVSFEMGV APFTGDGGVP
LLIYTVAYFS VVLFLVLNPG RLVDRVGKVI TPVLLSALLV LGGAAIFAPA GEIGSSSGEY
QSAPLVQGFL QGYLTMDTLG ALVFGIVIAT AIRDRGISDS RLVTRYSMIA GVIAATGLSL
VYLALFYLGA TSQGIAGDAQ NGVQILTAYV QQTFGVSGSL LLAVVITLAC LTTAVGLITA
CGEFFSDLLP VSYKTVVIVF SLFSLLVANQ GLTQLISLSV PVLVGLYPLA IVLIALSLFD
RLWVSAPRVF VPVMIVALLF GIVDGLGAAK LNGWVPDVFA KLPLADQSLG WLLPVSIALV
LAVVCDRLLG KPREAVA
