TEF_HUMAN
ID TEF_HUMAN Reviewed; 303 AA.
AC Q10587; B0QYS8; B2RC22; Q15729; Q7Z3J7; Q8IU94; Q96TG4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 3.
DT 03-AUG-2022, entry version 185.
DE RecName: Full=Thyrotroph embryonic factor;
GN Name=TEF; Synonyms=KIAA1655;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7835883; DOI=10.1006/geno.1994.1510;
RA Khatib Z.A., Inaba T., Valentine M., Look A.T.;
RT "Chromosomal localization and cDNA cloning of the human DBP and TEF
RT genes.";
RL Genomics 23:344-351(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8639829;
RA Hunger S.P., Li S., Fall M.Z., Naumovski L., Cleary M.L.;
RT "The proto-oncogene HLF and the related basic leucine zipper protein TEF
RT display highly similar DNA-binding and transcriptional regulatory
RT properties.";
RL Blood 87:4607-4617(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15461802; DOI=10.1186/gb-2004-5-10-r84;
RA Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A.,
RA Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J.,
RA Beare D.M., Dunham I.;
RT "A genome annotation-driven approach to cloning the human ORFeome.";
RL Genome Biol. 5:R84.1-R84.11(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P.,
RA Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y.,
RA LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Cerebellum, and Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10591208; DOI=10.1038/990031;
RA Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA Wright H.;
RT "The DNA sequence of human chromosome 22.";
RL Nature 402:489-495(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-303.
RC TISSUE=Retina;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
CC -!- FUNCTION: Transcription factor that binds to and transactivates the
CC TSHB promoter. Binds to a minimal DNA-binding sequence 5'-
CC [TC][AG][AG]TTA[TC][AG]-3'.
CC -!- SUBUNIT: Binds DNA as a homodimer or a heterodimer. Can form a
CC heterodimer with DBP.
CC -!- INTERACTION:
CC Q10587; P50570-2: DNM2; NbExp=3; IntAct=EBI-2796967, EBI-10968534;
CC Q10587; Q13084: MRPL28; NbExp=3; IntAct=EBI-2796967, EBI-723426;
CC Q10587; Q16236: NFE2L2; NbExp=5; IntAct=EBI-2796967, EBI-2007911;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q10587-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q10587-2; Sequence=VSP_041376;
CC -!- INDUCTION: Accumulates according to a robust circadian rhythm.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bZIP family. PAR subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA81373.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U06935; AAA81373.1; ALT_INIT; mRNA.
DR EMBL; U44059; AAB06497.1; -; mRNA.
DR EMBL; CR456592; CAG30478.1; -; mRNA.
DR EMBL; CR541827; CAG46626.1; -; mRNA.
DR EMBL; AK091916; BAG52441.1; -; mRNA.
DR EMBL; AK314906; BAG37419.1; -; mRNA.
DR EMBL; AL035659; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471095; EAW60430.1; -; Genomic_DNA.
DR EMBL; CH471095; EAW60431.1; -; Genomic_DNA.
DR EMBL; BC039258; AAH39258.1; -; mRNA.
DR EMBL; BC042476; AAH42476.1; -; mRNA.
DR EMBL; BX537848; CAD97856.1; -; mRNA.
DR CCDS; CCDS14014.1; -. [Q10587-1]
DR CCDS; CCDS46716.1; -. [Q10587-2]
DR PIR; G02360; G02360.
DR RefSeq; NP_001138870.1; NM_001145398.2. [Q10587-2]
DR RefSeq; NP_003207.1; NM_003216.3. [Q10587-1]
DR PDB; 4U5T; X-ray; 3.30 A; A/B=257-295.
DR PDBsum; 4U5T; -.
DR AlphaFoldDB; Q10587; -.
DR SMR; Q10587; -.
DR BioGRID; 112867; 3.
DR IntAct; Q10587; 33.
DR STRING; 9606.ENSP00000266304; -.
DR iPTMnet; Q10587; -.
DR PhosphoSitePlus; Q10587; -.
DR BioMuta; TEF; -.
DR DMDM; 51338730; -.
DR EPD; Q10587; -.
DR jPOST; Q10587; -.
DR MassIVE; Q10587; -.
DR PaxDb; Q10587; -.
DR PeptideAtlas; Q10587; -.
DR PRIDE; Q10587; -.
DR ProteomicsDB; 58863; -. [Q10587-1]
DR ProteomicsDB; 58864; -. [Q10587-2]
DR Antibodypedia; 26917; 176 antibodies from 25 providers.
DR DNASU; 7008; -.
DR Ensembl; ENST00000266304.9; ENSP00000266304.4; ENSG00000167074.15. [Q10587-1]
DR Ensembl; ENST00000406644.7; ENSP00000385256.3; ENSG00000167074.15. [Q10587-2]
DR GeneID; 7008; -.
DR KEGG; hsa:7008; -.
DR MANE-Select; ENST00000266304.9; ENSP00000266304.4; NM_003216.4; NP_003207.1.
DR UCSC; uc003azx.5; human. [Q10587-1]
DR CTD; 7008; -.
DR DisGeNET; 7008; -.
DR GeneCards; TEF; -.
DR HGNC; HGNC:11722; TEF.
DR HPA; ENSG00000167074; Low tissue specificity.
DR MIM; 188595; gene.
DR neXtProt; NX_Q10587; -.
DR OpenTargets; ENSG00000167074; -.
DR PharmGKB; PA36439; -.
DR VEuPathDB; HostDB:ENSG00000167074; -.
DR eggNOG; KOG3119; Eukaryota.
DR GeneTree; ENSGT00940000156578; -.
DR HOGENOM; CLU_051922_2_0_1; -.
DR InParanoid; Q10587; -.
DR OMA; PDCVEVD; -.
DR OrthoDB; 1023460at2759; -.
DR PhylomeDB; Q10587; -.
DR TreeFam; TF315869; -.
DR PathwayCommons; Q10587; -.
DR SignaLink; Q10587; -.
DR SIGNOR; Q10587; -.
DR BioGRID-ORCS; 7008; 31 hits in 1094 CRISPR screens.
DR ChiTaRS; TEF; human.
DR GeneWiki; TEF_(gene); -.
DR GenomeRNAi; 7008; -.
DR Pharos; Q10587; Tbio.
DR PRO; PR:Q10587; -.
DR Proteomes; UP000005640; Chromosome 22.
DR RNAct; Q10587; protein.
DR Bgee; ENSG00000167074; Expressed in cerebellar hemisphere and 179 other tissues.
DR ExpressionAtlas; Q10587; baseline and differential.
DR Genevisible; Q10587; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0044877; F:protein-containing complex binding; IEA:Ensembl.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR040223; PAR_bZIP.
DR InterPro; IPR029832; TEF/VBP.
DR PANTHER; PTHR11988; PTHR11988; 1.
DR PANTHER; PTHR11988:SF24; PTHR11988:SF24; 1.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; Biological rhythms;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..303
FT /note="Thyrotroph embryonic factor"
FT /id="PRO_0000076512"
FT DOMAIN 233..296
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 235..255
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 256..263
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 44..63
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..158
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 32
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT VAR_SEQ 1..52
FT /note="MSDAGGGKKPPVDPQAGPGPGPGRAAGERGLSGSFPLVLKKLMENPPREARL
FT -> MDMPEVLKSLLEHSLPWPEKRT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_041376"
FT CONFLICT 54
FT /note="K -> E (in Ref. 1; AAA81373)"
FT /evidence="ECO:0000305"
FT HELIX 261..293
FT /evidence="ECO:0007829|PDB:4U5T"
SQ SEQUENCE 303 AA; 33248 MW; 4A87B7BFA7248C6F CRC64;
MSDAGGGKKP PVDPQAGPGP GPGRAAGERG LSGSFPLVLK KLMENPPREA RLDKEKGKEK
LEEDEAAAAS TMAVSASLMP PIWDKTIPYD GESFHLEYMD LDEFLLENGI PASPTHLAHN
LLLPVAELEG KESASSSTAS PPSSSTAIFQ PSETVSSTES SLEKERETPS PIDPNCVEVD
VNFNPDPADL VLSSVPGGEL FNPRKHKFAE EDLKPQPMIK KAKKVFVPDE QKDEKYWTRR
KKNNVAAKRS RDARRLKENQ ITIRAAFLEK ENTALRTEVA ELRKEVGKCK TIVSKYETKY
GPL
