TEF_MOUSE
ID TEF_MOUSE Reviewed; 301 AA.
AC Q9JLC6; Q3U426; Q3UGF4; Q3UM49; Q3URC8; Q6QHT6; Q8C6I0; Q8VD02;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Thyrotroph embryonic factor;
GN Name=Tef;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
RC STRAIN=BALB/cJ; TISSUE=Brain;
RX PubMed=10799536; DOI=10.1074/jbc.275.19.14524;
RA Krueger D.A., Warner E.A., Dowd D.R.;
RT "Involvement of thyrotroph embryonic factor in calcium-mediated regulation
RT of gene expression.";
RL J. Biol. Chem. 275:14524-14531(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), AND ALTERNATIVE
RP PROMOTER USAGE.
RX PubMed=14702338; DOI=10.1074/jbc.m313822200;
RA Zhou J., Hoggatt A.M., Herring B.P.;
RT "Activation of the smooth muscle-specific telokin gene by thyrotroph
RT embryonic factor (TEF).";
RL J. Biol. Chem. 279:15929-15937(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS ALPHA AND BETA).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Hippocampus, Kidney, and Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS ALPHA AND 2).
RC STRAIN=FVB/N; TISSUE=Kidney, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP CIRCADIAN INDUCTION.
RX PubMed=8617210; DOI=10.1002/j.1460-2075.1996.tb00365.x;
RA Fonjallaz P., Ossipow V., Wanner G., Schibler U.;
RT "The two PAR leucine zipper proteins, TEF and DBP, display similar
RT circadian and tissue-specific expression, but have different target
RT promoter preferences.";
RL EMBO J. 15:351-362(1996).
RN [6]
RP INVOLVEMENT IN EPILEPSY.
RX PubMed=15175240; DOI=10.1101/gad.301404;
RA Gachon F., Fonjallaz P., Damiola F., Gos P., Kodama T., Zakany J.,
RA Duboule D., Petit B., Tafti M., Schibler U.;
RT "The loss of circadian PAR bZip transcription factors results in
RT epilepsy.";
RL Genes Dev. 18:1397-1412(2004).
CC -!- FUNCTION: Transcription factor that binds to and transactivates the
CC TSHB promoter. Binds to a minimal DNA-binding sequence 5'-
CC [TC][AG][AG]TTA[TC][AG]-3' (By similarity). Also activates the telokin
CC promoter in smooth muscle-specific and calcium-dependent manner.
CC {ECO:0000250}.
CC -!- SUBUNIT: Binds DNA as a homodimer or a heterodimer. Can form a
CC heterodimer with DBP (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage; Named isoforms=3;
CC Name=Alpha;
CC IsoId=Q9JLC6-1; Sequence=Displayed;
CC Name=Beta;
CC IsoId=Q9JLC6-2; Sequence=VSP_011245;
CC Name=2;
CC IsoId=Q9JLC6-3; Sequence=VSP_011246;
CC -!- TISSUE SPECIFICITY: Isoform Alpha and isoform Beta are expressed at
CC high levels in lung, bladder, kidney, gut and brain.
CC -!- INDUCTION: Accumulates according to a robust circadian rhythm in liver
CC and kidney. In liver nuclei, the amplitude of daily oscillation has
CC been estimated to be 9-fold. Expressed at nearly constant level in the
CC brain.
CC -!- MISCELLANEOUS: Mice deficient for all three PAR bZIP proteins (DBP, HLF
CC and TEF) display a dramatically shortened life span and are highly
CC susceptible to generalized spontaneous and audiogenic epilepsies (due
CC for example to the noise of a vacuum cleaner) that are frequently
CC lethal. The down-regulation of pyridoxal kinase (Pdxk) expression in
CC these mice may participate in this seizure phenotype.
CC -!- SIMILARITY: Belongs to the bZIP family. PAR subfamily. {ECO:0000305}.
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DR EMBL; AF194420; AAF70830.1; -; mRNA.
DR EMBL; AY540631; AAS45599.1; -; mRNA.
DR EMBL; AY540632; AAS45600.1; -; mRNA.
DR EMBL; AK075601; BAC35849.1; -; mRNA.
DR EMBL; AK141606; BAE24760.1; -; mRNA.
DR EMBL; AK145125; BAE26249.1; -; mRNA.
DR EMBL; AK147965; BAE28255.1; -; mRNA.
DR EMBL; AK154472; BAE32609.1; -; mRNA.
DR EMBL; BC017689; AAH17689.1; -; mRNA.
DR EMBL; BC036982; AAH36982.1; -; mRNA.
DR CCDS; CCDS27671.2; -. [Q9JLC6-2]
DR CCDS; CCDS27672.1; -. [Q9JLC6-1]
DR RefSeq; NP_059072.1; NM_017376.3. [Q9JLC6-1]
DR RefSeq; NP_705617.2; NM_153484.3. [Q9JLC6-2]
DR AlphaFoldDB; Q9JLC6; -.
DR SMR; Q9JLC6; -.
DR BioGRID; 204106; 3.
DR IntAct; Q9JLC6; 2.
DR STRING; 10090.ENSMUSP00000023024; -.
DR iPTMnet; Q9JLC6; -.
DR PhosphoSitePlus; Q9JLC6; -.
DR EPD; Q9JLC6; -.
DR MaxQB; Q9JLC6; -.
DR PaxDb; Q9JLC6; -.
DR PRIDE; Q9JLC6; -.
DR ProteomicsDB; 263030; -. [Q9JLC6-1]
DR ProteomicsDB; 263031; -. [Q9JLC6-2]
DR ProteomicsDB; 263032; -. [Q9JLC6-3]
DR Antibodypedia; 26917; 176 antibodies from 25 providers.
DR DNASU; 21685; -.
DR Ensembl; ENSMUST00000023024; ENSMUSP00000023024; ENSMUSG00000022389. [Q9JLC6-1]
DR Ensembl; ENSMUST00000109553; ENSMUSP00000105180; ENSMUSG00000022389. [Q9JLC6-2]
DR GeneID; 21685; -.
DR KEGG; mmu:21685; -.
DR UCSC; uc007wxj.2; mouse. [Q9JLC6-2]
DR UCSC; uc007wxk.2; mouse. [Q9JLC6-1]
DR CTD; 7008; -.
DR MGI; MGI:98663; Tef.
DR VEuPathDB; HostDB:ENSMUSG00000022389; -.
DR eggNOG; KOG3119; Eukaryota.
DR GeneTree; ENSGT00940000156578; -.
DR HOGENOM; CLU_051922_2_0_1; -.
DR InParanoid; Q9JLC6; -.
DR OMA; PDCVEVD; -.
DR OrthoDB; 1023460at2759; -.
DR PhylomeDB; Q9JLC6; -.
DR TreeFam; TF315869; -.
DR BioGRID-ORCS; 21685; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Tef; mouse.
DR PRO; PR:Q9JLC6; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9JLC6; protein.
DR Bgee; ENSMUSG00000022389; Expressed in superior frontal gyrus and 262 other tissues.
DR ExpressionAtlas; Q9JLC6; baseline and differential.
DR Genevisible; Q9JLC6; MM.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR040223; PAR_bZIP.
DR InterPro; IPR029832; TEF/VBP.
DR PANTHER; PTHR11988; PTHR11988; 1.
DR PANTHER; PTHR11988:SF24; PTHR11988:SF24; 1.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative promoter usage; Biological rhythms; DNA-binding;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..301
FT /note="Thyrotroph embryonic factor"
FT /id="PRO_0000076513"
FT DOMAIN 231..294
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 130..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 233..253
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 254..261
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 42..64
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 130..156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 30
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q10587"
FT VAR_SEQ 1..50
FT /note="MSDAGGGKKPPVEPQAGPGPGRAAGERGLSGSFPLVLKKLMENPPRETRL
FT -> MDMPEVLKSLLEHSLPWSEKKA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_011246"
FT VAR_SEQ 1..50
FT /note="MSDAGGGKKPPVEPQAGPGPGRAAGERGLSGSFPLVLKKLMENPPRETRL
FT -> MSSCSQIGVAPAMDMPEVLKSLLEHSLPWSEKKA (in isoform Beta)"
FT /evidence="ECO:0000303|PubMed:14702338,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_011245"
FT CONFLICT 38
FT /note="K -> R (in Ref. 3; BAE26249)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="M -> V (in Ref. 3; BAC35849)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="S -> C (in Ref. 3; BAE28255)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="K -> E (in Ref. 3; BAE26249)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="F -> L (in Ref. 3; BAE26249)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 301 AA; 33145 MW; F83FFFC6D7E091A4 CRC64;
MSDAGGGKKP PVEPQAGPGP GRAAGERGLS GSFPLVLKKL MENPPRETRL DKEKGKEKLE
EDESAAASTM AVSASLMPPI WDKTIPYDGE SFHLEYMDLD EFLLENGIPA SPTHLAQNLL
LPVAELEGKE SASSSTASPP SSSTAIFQPS ETVSSTESSL EKERETPSPI DPSCVEVDVN
FNPDPADLVL SSVPGGELFN PRKHRFAEED LKPQPMIKKA KKVFVPDEQK DEKYWTRRKK
NNVAAKRSRD ARRLKENQIT IRAAFLEKEN TALRTEVAEL RKEVGKCKTI VSKYETKYGP
L
