BRAC_PSEAE
ID BRAC_PSEAE Reviewed; 373 AA.
AC P21175;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein;
DE Short=LIVAT-BP;
DE Short=Leu/Ile/Val/Thr/Ala-binding protein;
DE Flags: Precursor;
GN Name=braC; OrderedLocusNames=PA1074;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-39, AND FUNCTION.
RC STRAIN=PAO;
RX PubMed=2509433; DOI=10.1128/jb.171.11.6300-6306.1989;
RA Hoshino T., Kose K.;
RT "Cloning and nucleotide sequence of braC, the structural gene for the
RT leucine-, isoleucine-, and valine-binding protein of Pseudomonas aeruginosa
RT PAO.";
RL J. Bacteriol. 171:6300-6306(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=2120183; DOI=10.1128/jb.172.10.5531-5539.1990;
RA Hoshino T., Kose K.;
RT "Cloning, nucleotide sequences, and identification of products of the
RT Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity
RT branched-chain amino acid transport system.";
RL J. Bacteriol. 172:5531-5539(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [4]
RP FUNCTION AS A BINDING PROTEIN.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=31627455; DOI=10.3390/ijms20205156;
RA Fernandez M., Rico-Jimenez M., Ortega A., Daddaoua A., Garcia Garcia A.I.,
RA Martin-Mora D., Torres N.M., Tajuelo A., Matilla M.A., Krell T.;
RT "Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding
RT Proteins.";
RL Int. J. Mol. Sci. 20:5156-5156(2019).
CC -!- FUNCTION: Component of the high-affinity leucine, isoleucine, valine
CC transport system I (LIV-I), which is operative without Na(+) and is
CC specific for alanine and threonine, in addition to branched-chain amino
CC acids (PubMed:2509433). Binds L-leucine, L-isoleucine, L-valine, L-
CC threonine and L-alanine with nanomolar affinities. Can also bind L-
CC homoserine with high affinity (PubMed:31627455).
CC {ECO:0000269|PubMed:2509433, ECO:0000269|PubMed:31627455}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the leucine-binding protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M31071; AAA88430.1; -; Genomic_DNA.
DR EMBL; D90223; BAA14254.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04463.1; -; Genomic_DNA.
DR PIR; A36125; A36125.
DR RefSeq; NP_249765.1; NC_002516.2.
DR RefSeq; WP_003082136.1; NZ_QZGE01000006.1.
DR AlphaFoldDB; P21175; -.
DR SMR; P21175; -.
DR STRING; 287.DR97_869; -.
DR TCDB; 3.A.1.4.8; the atp-binding cassette (abc) superfamily.
DR PaxDb; P21175; -.
DR PRIDE; P21175; -.
DR EnsemblBacteria; AAG04463; AAG04463; PA1074.
DR GeneID; 878501; -.
DR KEGG; pae:PA1074; -.
DR PATRIC; fig|208964.12.peg.1112; -.
DR PseudoCAP; PA1074; -.
DR HOGENOM; CLU_027128_6_0_6; -.
DR InParanoid; P21175; -.
DR OMA; MEFTGAR; -.
DR PhylomeDB; P21175; -.
DR BioCyc; PAER208964:G1FZ6-1097-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0015803; P:branched-chain amino acid transport; IDA:PseudoCAP.
DR GO; GO:0042941; P:D-alanine transport; IMP:PseudoCAP.
DR GO; GO:0015808; P:L-alanine transport; IMP:PseudoCAP.
DR GO; GO:1903806; P:L-isoleucine import across plasma membrane; IMP:PseudoCAP.
DR GO; GO:1903805; P:L-valine import across plasma membrane; IMP:PseudoCAP.
DR InterPro; IPR028081; Leu-bd.
DR InterPro; IPR000709; Leu_Ile_Val-bd.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF13458; Peripla_BP_6; 1.
DR PRINTS; PR00337; LEUILEVALBP.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Direct protein sequencing; Disulfide bond; Periplasm;
KW Reference proteome; Signal; Transport.
FT SIGNAL 1..26
FT /evidence="ECO:0000269|PubMed:2509433"
FT CHAIN 27..373
FT /note="Leucine-, isoleucine-, valine-, threonine-, and
FT alanine-binding protein"
FT /id="PRO_0000017698"
FT DISULFID 80..105
FT CONFLICT 165
FT /note="D -> V (in Ref. 1; AAA88430)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 373 AA; 39769 MW; 105188ADDEDEE63B CRC64;
MKKGTQRLSR LFAAMAIAGF ASYSMAADTI KIALAGPVTG PVAQYGDMQR AGALMAIEQI
NKAGGVNGAQ LEGVIYDDAC DPKQAVAVAN KVVNDGVKFV VGHVCSSSTQ PATDIYEDEG
VLMITPSATA PEITSRGYKL IFRTIGLDNM QGPVAGKFIA ERYKDKTIAV LHDKQQYGEG
IATEVKKTVE DAGIKVAVFE GLNAGDKDFN ALISKLKKAG VQFVYFGGYH PEMGLLLRQA
KQAGLDARFM GPEGVGNSEI TAIAGDASEG MLATLPRAFE QDPKNKALID AFKAKNQDPS
GIFVLPAYSA VTVIAKGIEK AGEADPEKVA EALRANTFET PTGNLGFDEK GDLKNFDFTV
YEWHKDATRT EVK
