BRAD_PSEAE
ID BRAD_PSEAE Reviewed; 307 AA.
AC P21627; Q6LDM2;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=High-affinity branched-chain amino acid transport system permease protein BraD;
GN Name=braD; OrderedLocusNames=PA1073;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=2120183; DOI=10.1128/jb.172.10.5531-5539.1990;
RA Hoshino T., Kose K.;
RT "Cloning, nucleotide sequences, and identification of products of the
RT Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity
RT branched-chain amino acid transport system.";
RL J. Bacteriol. 172:5531-5539(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
RC STRAIN=PAO;
RX PubMed=2509433; DOI=10.1128/jb.171.11.6300-6306.1989;
RA Hoshino T., Kose K.;
RT "Cloning and nucleotide sequence of braC, the structural gene for the
RT leucine-, isoleucine-, and valine-binding protein of Pseudomonas aeruginosa
RT PAO.";
RL J. Bacteriol. 171:6300-6306(1989).
CC -!- FUNCTION: Component of the high affinity leucine, isoleucine, valine,
CC transport system (LIV-I), which is operative without Na(+) and is
CC specific for alanine and threonine, in addition to branched-chain amino
CC acids.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. LivHM subfamily. {ECO:0000305}.
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DR EMBL; D90223; BAA14255.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04462.1; -; Genomic_DNA.
DR EMBL; M31071; AAA88431.1; -; Genomic_DNA.
DR PIR; B36125; B36125.
DR RefSeq; NP_249764.1; NC_002516.2.
DR RefSeq; WP_003086391.1; NZ_QZGE01000006.1.
DR AlphaFoldDB; P21627; -.
DR STRING; 287.DR97_870; -.
DR TCDB; 3.A.1.4.8; the atp-binding cassette (abc) superfamily.
DR PaxDb; P21627; -.
DR PRIDE; P21627; -.
DR DNASU; 881243; -.
DR EnsemblBacteria; AAG04462; AAG04462; PA1073.
DR GeneID; 881243; -.
DR KEGG; pae:PA1073; -.
DR PATRIC; fig|208964.12.peg.1111; -.
DR PseudoCAP; PA1073; -.
DR HOGENOM; CLU_039929_3_0_6; -.
DR InParanoid; P21627; -.
DR OMA; NMGWFLI; -.
DR PhylomeDB; P21627; -.
DR BioCyc; PAER208964:G1FZ6-1096-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015190; F:L-leucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015192; F:L-phenylalanine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042941; P:D-alanine transport; IMP:PseudoCAP.
DR GO; GO:0015808; P:L-alanine transport; IMP:PseudoCAP.
DR GO; GO:1903806; P:L-isoleucine import across plasma membrane; IMP:PseudoCAP.
DR GO; GO:1903785; P:L-valine transmembrane transport; IBA:GO_Central.
DR GO; GO:0098713; P:leucine import across plasma membrane; IBA:GO_Central.
DR GO; GO:0015823; P:phenylalanine transport; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR001851; ABC_transp_permease.
DR Pfam; PF02653; BPD_transp_2; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..307
FT /note="High-affinity branched-chain amino acid transport
FT system permease protein BraD"
FT /id="PRO_0000059966"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 307 AA; 32514 MW; 362AE5D3A931A77E CRC64;
MPEIYHYLQQ LVNGLTVGST YALIAIGYTM VYGIIGMINF AHGEVYMIGS YIAFIAITLL
AMMGLDSVPL MMLAAFAASI IVTSAFGYSI ERVAYRPLRG GNRLIPLISA IGMSIFLQNA
VMLSQDSKEK AIPTLLPGNF VFGESSMNGV VISYMQILIF VVTFLVMFGL TLFISRSRLG
RACRACAEDL KMTNLLGINS NNIIALTFVI GAALAAVAAV LLGMQYGVIN PGIGFLAGIK
AFTAAVLGGI GSIPGAMLGG LLLGVAEAFG ADVFGDQYKD VVAFGLLILV LLFRPTGILG
RPEVEKV
