BRAE_PSEAE
ID BRAE_PSEAE Reviewed; 417 AA.
AC P21628;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=High-affinity branched-chain amino acid transport system permease protein BraE;
GN Name=braE; OrderedLocusNames=PA1072;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=2120183; DOI=10.1128/jb.172.10.5531-5539.1990;
RA Hoshino T., Kose K.;
RT "Cloning, nucleotide sequences, and identification of products of the
RT Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity
RT branched-chain amino acid transport system.";
RL J. Bacteriol. 172:5531-5539(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Component of the high affinity leucine, isoleucine, valine,
CC transport system (LIV-I), which is operative without Na(+) and is
CC specific for alanine and threonine, in addition to branched-chain amino
CC acids.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. LivHM subfamily. {ECO:0000305}.
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DR EMBL; D90223; BAA14256.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04461.1; -; Genomic_DNA.
DR PIR; C36125; C36125.
DR RefSeq; NP_249763.1; NC_002516.2.
DR RefSeq; WP_003082131.1; NZ_QZGE01000006.1.
DR AlphaFoldDB; P21628; -.
DR STRING; 287.DR97_871; -.
DR TCDB; 3.A.1.4.8; the atp-binding cassette (abc) superfamily.
DR PaxDb; P21628; -.
DR PRIDE; P21628; -.
DR EnsemblBacteria; AAG04461; AAG04461; PA1072.
DR GeneID; 880501; -.
DR KEGG; pae:PA1072; -.
DR PATRIC; fig|208964.12.peg.1110; -.
DR PseudoCAP; PA1072; -.
DR HOGENOM; CLU_031365_1_1_6; -.
DR InParanoid; P21628; -.
DR OMA; VFWYKLQ; -.
DR PhylomeDB; P21628; -.
DR BioCyc; PAER208964:G1FZ6-1095-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015658; F:branched-chain amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015803; P:branched-chain amino acid transport; IBA:GO_Central.
DR GO; GO:0042941; P:D-alanine transport; IMP:PseudoCAP.
DR CDD; cd06581; TM_PBP1_LivM_like; 1.
DR InterPro; IPR001851; ABC_transp_permease.
DR InterPro; IPR021807; LivHM_N.
DR InterPro; IPR043428; LivM-like.
DR PANTHER; PTHR30482; PTHR30482; 1.
DR Pfam; PF02653; BPD_transp_2; 1.
DR Pfam; PF11862; DUF3382; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..417
FT /note="High-affinity branched-chain amino acid transport
FT system permease protein BraE"
FT /id="PRO_0000059967"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 417 AA; 45562 MW; 26F5E4C20DBF7AF1 CRC64;
MSQSLKRALF SALLVILVSY PILGLKLRTV GIKLEVLGAD AQTLWTIAAA ALAMFVWQLF
RDRIPLKLGR GVGYKVNGSG LKNFLSLPST QRWAVLALVV VAFVWPFFAS RGAVDIATLI
LIYVMLGIGL NIVVGLAGLL DLGYVGFYAV GAYTYALLAE YAGFGFWTAL PIAGMMAALF
GFLLGFPVLR LRGDYLAIVT LGFGEIIRIL LRNMTEITGG PNGIGSIPKP TLFGLTFERR
APEGMQTFHE FFGIAYNTNY KVILLYVVAL LLVLLALFVI NRLMRMPIGR AWEALREDEV
ACRALGLNPT IVKLSAFTIG ASFAGFAGSF FAARQGLVTP ESFTFIESAM ILAIVVLGGM
GSQLGVILAA VVMVLLQEMR GFNEYRMLIF GLTMIVMMIW RPQGLLPMQR PHLELKP
