TEKT2_MOUSE
ID TEKT2_MOUSE Reviewed; 430 AA.
AC Q922G7; Q9WVR0;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Tektin-2;
DE AltName: Full=Tektin-t;
DE AltName: Full=Testicular tektin;
GN Name=Tekt2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Testis;
RX PubMed=10456331; DOI=10.1016/s0014-5793(99)00967-9;
RA Iguchi N., Tanaka H., Fujii T., Tamura K., Kaneko Y., Nojima H.,
RA Nishimune Y.;
RT "Molecular cloning of haploid germ cell-specific tektin cDNA and analysis
RT of the protein in mouse testis.";
RL FEBS Lett. 456:315-321(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15340058; DOI=10.1128/mcb.24.18.7958-7964.2004;
RA Tanaka H., Iguchi N., Toyama Y., Kitamura K., Takahashi T., Kaseda K.,
RA Maekawa M., Nishimune Y.;
RT "Mice deficient in the axonemal protein tektin-t exhibit male infertility
RT and immotile-cilium syndrome due to impaired inner arm dynein function.";
RL Mol. Cell. Biol. 24:7958-7964(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH CCDC172.
RX PubMed=24394471; DOI=10.1369/0022155413520607;
RA Yamaguchi A., Kaneko T., Inai T., Iida H.;
RT "Molecular cloning and subcellular localization of Tektin2-binding protein
RT 1 (Ccdc 172) in rat spermatozoa.";
RL J. Histochem. Cytochem. 62:286-297(2014).
CC -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC doublet microtubules (DMTs) in cilia and flagellar axoneme
CC (PubMed:15340058). Plays a key role in the assembly or attachment of
CC the inner dynein arm to microtubules in sperm flagella and tracheal
CC cilia (PubMed:15340058). Forms filamentous polymers in the walls of
CC ciliary and flagellar microtubules (PubMed:15340058).
CC {ECO:0000269|PubMed:15340058}.
CC -!- SUBUNIT: May interact with CCDC172. {ECO:0000269|PubMed:24394471}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:15340058}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000269|PubMed:10456331}. Cytoplasm, cytoskeleton,
CC microtubule organizing center {ECO:0000269|PubMed:24394471}.
CC Note=Colocalized with CCDC172 at the perinuclear region
CC (PubMed:24394471). {ECO:0000269|PubMed:24394471}.
CC -!- TISSUE SPECIFICITY: Testis specific. {ECO:0000269|PubMed:10456331}.
CC -!- DEVELOPMENTAL STAGE: Localized in the flagella of elongating spermatids
CC from developmental step 15 to maturity. {ECO:0000269|PubMed:10456331}.
CC -!- PTM: Tyrosine phosphorylated. {ECO:0000250|UniProtKB:Q1W6C3}.
CC -!- SIMILARITY: Belongs to the tektin family. {ECO:0000305}.
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DR EMBL; AB027138; BAA81822.1; -; mRNA.
DR EMBL; BC008140; AAH08140.1; -; mRNA.
DR CCDS; CCDS18651.1; -.
DR RefSeq; NP_036032.2; NM_011902.2.
DR RefSeq; XP_017175679.1; XM_017320190.1.
DR RefSeq; XP_017175680.1; XM_017320191.1.
DR RefSeq; XP_017175681.1; XM_017320192.1.
DR AlphaFoldDB; Q922G7; -.
DR SMR; Q922G7; -.
DR BioGRID; 204892; 1.
DR STRING; 10090.ENSMUSP00000099676; -.
DR PhosphoSitePlus; Q922G7; -.
DR MaxQB; Q922G7; -.
DR PaxDb; Q922G7; -.
DR PRIDE; Q922G7; -.
DR ProteomicsDB; 262865; -.
DR Antibodypedia; 17404; 146 antibodies from 23 providers.
DR DNASU; 24084; -.
DR Ensembl; ENSMUST00000030658; ENSMUSP00000030658; ENSMUSG00000028845.
DR Ensembl; ENSMUST00000102616; ENSMUSP00000099676; ENSMUSG00000028845.
DR GeneID; 24084; -.
DR KEGG; mmu:24084; -.
DR UCSC; uc008utf.1; mouse.
DR CTD; 27285; -.
DR MGI; MGI:1346335; Tekt2.
DR VEuPathDB; HostDB:ENSMUSG00000028845; -.
DR eggNOG; KOG2685; Eukaryota.
DR GeneTree; ENSGT00950000182894; -.
DR HOGENOM; CLU_033588_0_0_1; -.
DR InParanoid; Q922G7; -.
DR OMA; FDHRGKM; -.
DR OrthoDB; 581072at2759; -.
DR PhylomeDB; Q922G7; -.
DR TreeFam; TF320754; -.
DR BioGRID-ORCS; 24084; 4 hits in 73 CRISPR screens.
DR PRO; PR:Q922G7; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q922G7; protein.
DR Bgee; ENSMUSG00000028845; Expressed in testis and 62 other tissues.
DR ExpressionAtlas; Q922G7; baseline and differential.
DR Genevisible; Q922G7; MM.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0015630; C:microtubule cytoskeleton; IBA:GO_Central.
DR GO; GO:0005815; C:microtubule organizing center; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IBA:GO_Central.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR GO; GO:0036159; P:inner dynein arm assembly; IMP:MGI.
DR InterPro; IPR000435; Tektin.
DR PANTHER; PTHR19960; PTHR19960; 1.
DR PRINTS; PR00511; TEKTIN.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Cytoplasm; Cytoskeleton; Flagellum; Microtubule; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..430
FT /note="Tektin-2"
FT /id="PRO_0000184566"
FT COILED 75..162
FT /evidence="ECO:0000255"
FT COILED 226..380
FT /evidence="ECO:0000255"
FT CONFLICT 32
FT /note="R -> Q (in Ref. 1; BAA81822)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 430 AA; 50311 MW; 1FE35ED77B5E9CDB CRC64;
MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN
RTRLAERIDT VNRWKETLDK CLTDLDAEID SLAQAKESAE QNLQAKNLPL DVAIECLTLR
ESRRDIDVVR DPVEEELLKE VEVIEATKKV LQEKISQAFQ HLCLLQEIRQ QLNSDHRDKM
ETLEIDRGCL SLNLTSPNIS LKVNPTRIPK DSTTLQQWDE FTRFNKNRAE AEMKASIELR
EAIALAIAQT NNELDAQRVA TEFTFRKRLR EMESFYSELK WQEKNTLEEI AELQGDIRRL
EEDLRRKMMN LKLAHTRLES RTYRSNVELC RDQTQYGLID EVHQLEATIN TMKQKLAQTQ
NALDALFKHL ARIQADIACK TNTLLLDTKC MDTRRKLTVP AEKFVPQVDT FTRTTNRTLS
PLKICQLELT
