TEKT4_MOUSE
ID TEKT4_MOUSE Reviewed; 447 AA.
AC Q149S1; A0A0R4J051; Q3ZTL6; Q9DAH3;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Tektin-4;
DE AltName: Full=Testicular microtubules-related protein 4;
GN Name=Tekt4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6 X 129;
RX PubMed=17244819; DOI=10.1096/fj.06-7035com;
RA Roy A., Lin Y.N., Agno J.E., DeMayo F.J., Matzuk M.M.;
RT "Absence of tektin 4 causes asthenozoospermia and subfertility in male
RT mice.";
RL FASEB J. 21:1013-1025(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16596631; DOI=10.1002/mrd.20486;
RA Iida H., Honda Y., Matsuyama T., Shibata Y., Inai T.;
RT "Tektin 4 is located on outer dense fibers, not associated with axonemal
RT tubulins of flagella in rodent spermatozoa.";
RL Mol. Reprod. Dev. 73:929-936(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms
CC filamentous polymers in the walls of ciliary and flagellar microtubules
CC (By similarity). Contributes to normal sperm motility
CC (PubMed:17244819). {ECO:0000250|UniProtKB:Q2TA38,
CC ECO:0000269|PubMed:17244819}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:Q2TA38}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:16596631, ECO:0000269|PubMed:17244819}. Note=Found
CC in the abaxial (convex) surface of outer dense fibers in sperm
CC flagella. {ECO:0000269|PubMed:16596631}.
CC -!- TISSUE SPECIFICITY: Detected in testis, where it is weakly expressed in
CC round spermatids, and strongly expressed in the flagellum of step 16
CC elongated spermatids (at protein level) (PubMed:17244819). In the sperm
CC flagellum, localizes to the principal piece and midpiece (at protein
CC level) (PubMed:17244819, PubMed:16596631). Specifically expressed in
CC testis; not detected in other tissues tested (PubMed:17244819).
CC {ECO:0000269|PubMed:16596631, ECO:0000269|PubMed:17244819}.
CC -!- DEVELOPMENTAL STAGE: Detected in testis from postnatal day 16 onwards,
CC reaching maximal levels by postnatal day 18.
CC {ECO:0000269|PubMed:17244819}.
CC -!- DISRUPTION PHENOTYPE: In a 129S5/SvEvBrd genetic background, males show
CC progressive reduction in fertility with almost complete loss of
CC fertility after 5 months of breeding. Testis weight and histology
CC appear normal. Spermatozoa have significantly reduced forward motility.
CC The sperm flagellum shows defective bending in the midpiece region
CC which impairs waveform propagation and forward propulsion. Sperm ATP
CC levels deplete significantly over time, probably as a result of excess
CC energy consumption from inefficient flagellar beating. The
CC ultrastructure of the flagellum has some subtle abnormalities with an
CC enlarged space between the mitochondrial sheath and the outer dense
CC fibers. In a mixed C57BL/6J;129S5/SvEvBrd genetic background, male
CC fertility is not significantly affected. {ECO:0000269|PubMed:17244819}.
CC -!- SIMILARITY: Belongs to the tektin family. {ECO:0000305}.
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DR EMBL; AY485267; AAS55789.1; -; mRNA.
DR EMBL; AK005842; BAB24268.1; -; mRNA.
DR EMBL; AC131323; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC117510; AAI17511.1; -; mRNA.
DR EMBL; BC117526; AAI17527.1; -; mRNA.
DR CCDS; CCDS28519.1; -.
DR RefSeq; NP_082227.1; NM_027951.1.
DR AlphaFoldDB; Q149S1; -.
DR SMR; Q149S1; -.
DR BioGRID; 214969; 1.
DR STRING; 10090.ENSMUSP00000025002; -.
DR iPTMnet; Q149S1; -.
DR PhosphoSitePlus; Q149S1; -.
DR REPRODUCTION-2DPAGE; Q149S1; -.
DR PaxDb; Q149S1; -.
DR PRIDE; Q149S1; -.
DR ProteomicsDB; 262866; -.
DR ProteomicsDB; 368063; -.
DR Antibodypedia; 32286; 176 antibodies from 22 providers.
DR Ensembl; ENSMUST00000025002; ENSMUSP00000025002; ENSMUSG00000024175.
DR GeneID; 71840; -.
DR KEGG; mmu:71840; -.
DR UCSC; uc008baw.1; mouse.
DR CTD; 150483; -.
DR MGI; MGI:1919090; Tekt4.
DR VEuPathDB; HostDB:ENSMUSG00000024175; -.
DR eggNOG; KOG2685; Eukaryota.
DR GeneTree; ENSGT00950000182894; -.
DR InParanoid; Q149S1; -.
DR OMA; RNLEDTH; -.
DR OrthoDB; 581072at2759; -.
DR PhylomeDB; Q149S1; -.
DR TreeFam; TF320754; -.
DR BioGRID-ORCS; 71840; 4 hits in 71 CRISPR screens.
DR PRO; PR:Q149S1; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q149S1; protein.
DR Bgee; ENSMUSG00000024175; Expressed in spermatid and 41 other tissues.
DR ExpressionAtlas; Q149S1; baseline and differential.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0015630; C:microtubule cytoskeleton; IBA:GO_Central.
DR GO; GO:0036126; C:sperm flagellum; IDA:MGI.
DR GO; GO:0097225; C:sperm midpiece; IDA:MGI.
DR GO; GO:0097228; C:sperm principal piece; IDA:MGI.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IMP:MGI.
DR GO; GO:0060378; P:regulation of brood size; IGI:UniProtKB.
DR InterPro; IPR000435; Tektin.
DR PANTHER; PTHR19960; PTHR19960; 1.
DR PRINTS; PR00511; TEKTIN.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Cytoplasm; Cytoskeleton; Flagellum; Reference proteome.
FT CHAIN 1..447
FT /note="Tektin-4"
FT /id="PRO_0000261163"
FT COILED 322..348
FT /evidence="ECO:0000255"
FT COILED 375..423
FT /evidence="ECO:0000255"
FT CONFLICT 262
FT /note="H -> Q (in Ref. 4; AAI17511/AAI17527)"
FT CONFLICT 434
FT /note="R -> S (in Ref. 2; BAB24268)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 447 AA; 52045 MW; CBE31B4B53A8CEF7 CRC64;
MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS
YARYHQAFAD RDYSERQRHE SGQLVAETGA LAQRTQLDST RKVGERLEDM HCWKSELQRE
IDELSSETDL MMAQKLRLQR ALDATSVPYS IATDNLQCRE RRQHPDLVRD YVEVELLKET
ELIRNIQELL KRTIGQAVDQ IRLNREHKES CEMNWSDKVE VYNIDDTCSR YTNESTQVQF
YPHSSKFEES ASTPETWAKF NHDNLLRAER ERLASVNLRK LIDCILRDTA EDLRLQCDAV
NSAFSSRCQE LDDSLQKLQY HLRKTLTEIT DQEHQIAALK QAIKDKEAPL RVAQTRLYQR
SHRPNVELCR DNAQFRLLSE VEELNMSLRA LKEKLQDAEQ ALRNLEDSRM SLEKDIAVKT
NSLFIDRQKC MTHRNRYPSV LQLAGYQ
