BRAF_PSEAE
ID BRAF_PSEAE Reviewed; 255 AA.
AC P21629;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=High-affinity branched-chain amino acid transport ATP-binding protein BraF;
GN Name=braF; OrderedLocusNames=PA1071;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=2120183; DOI=10.1128/jb.172.10.5531-5539.1990;
RA Hoshino T., Kose K.;
RT "Cloning, nucleotide sequences, and identification of products of the
RT Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity
RT branched-chain amino acid transport system.";
RL J. Bacteriol. 172:5531-5539(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Component of the high affinity leucine, isoleucine, valine,
CC transport system (LIV-I), which is operative without Na(+) and is
CC specific for alanine and threonine, in addition to branched-chain amino
CC acids.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Peripheral
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR EMBL; D90223; BAA14257.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04460.1; -; Genomic_DNA.
DR PIR; D36125; D36125.
DR RefSeq; NP_249762.1; NC_002516.2.
DR RefSeq; WP_003082125.1; NZ_QZGE01000006.1.
DR AlphaFoldDB; P21629; -.
DR SMR; P21629; -.
DR STRING; 287.DR97_872; -.
DR TCDB; 3.A.1.4.8; the atp-binding cassette (abc) superfamily.
DR PaxDb; P21629; -.
DR PRIDE; P21629; -.
DR DNASU; 878078; -.
DR EnsemblBacteria; AAG04460; AAG04460; PA1071.
DR GeneID; 878078; -.
DR KEGG; pae:PA1071; -.
DR PATRIC; fig|208964.12.peg.1109; -.
DR PseudoCAP; PA1071; -.
DR HOGENOM; CLU_000604_1_2_6; -.
DR InParanoid; P21629; -.
DR OMA; EHDMRFI; -.
DR PhylomeDB; P21629; -.
DR BioCyc; PAER208964:G1FZ6-1094-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015190; F:L-leucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015192; F:L-phenylalanine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042941; P:D-alanine transport; IMP:PseudoCAP.
DR GO; GO:0015808; P:L-alanine transport; IMP:PseudoCAP.
DR GO; GO:1903806; P:L-isoleucine import across plasma membrane; IMP:PseudoCAP.
DR GO; GO:1903805; P:L-valine import across plasma membrane; IMP:PseudoCAP.
DR GO; GO:0098713; P:leucine import across plasma membrane; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR032823; BCA_ABC_TP_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF12399; BCA_ABC_TP_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; ATP-binding; Cell inner membrane; Cell membrane;
KW Membrane; Nucleotide-binding; Reference proteome; Transport.
FT CHAIN 1..255
FT /note="High-affinity branched-chain amino acid transport
FT ATP-binding protein BraF"
FT /id="PRO_0000091948"
FT DOMAIN 6..254
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 255 AA; 28284 MW; E9FCA1D448D2847A CRC64;
MSRPILEVSG LTMRFGGLLA VNGVNLKVEE KQVVSMIGPN GAGKTTVFNC LTGFYQPTGG
LIRLDGEEIQ GLPGHKIARK GVVRTFQNVR LFKEMTAVEN LLVAQHRHLN TNFLAGLFKT
PAFRRSEREA MEYAAHWLEE VNLTEFANRS AGTLAYGQQR RLEIARCMMT RPRILMLDEP
AAGLNPKETD DLKALIAKLR SEHNVTVLLI EHDMKLVMSI SDHIVVINQG APLADGTPEQ
IRDNPDVIKA YLGEA