TEL2_SCHPO
ID TEL2_SCHPO Reviewed; 868 AA.
AC Q9P3W5;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=DNA replication checkpoint protein tel2;
GN Name=tel2; ORFNames=SPAC458.03;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=17189249; DOI=10.1074/jbc.m607432200;
RA Shikata M., Ishikawa F., Kanoh J.;
RT "Tel2 is required for activation of the Mrc1-mediated replication
RT checkpoint.";
RL J. Biol. Chem. 282:5346-5355(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP IDENTIFICATION IN THE TORC1 AND TORC2 COMPLEXES, INTERACTION WITH TTI1, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18076573; DOI=10.1111/j.1365-2443.2007.01141.x;
RA Hayashi T., Hatanaka M., Nagao K., Nakaseko Y., Kanoh J., Kokubu A.,
RA Ebe M., Yanagida M.;
RT "Rapamycin sensitivity of the Schizosaccharomyces pombe tor2 mutant and
RT organization of two highly phosphorylated TOR complexes by specific and
RT common subunits.";
RL Genes Cells 12:1357-1370(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-490, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Component of the TORC1 and TORC2 complexes required for the
CC regulation of the cellular respons to changes in available nutrients.
CC Required for normal entry into S phase. Regulates activation of the DNA
CC replication checkpoint. Required for efficient phosphorylation of mrc1,
CC which is essential for the activation of cds1 kinase. Also plays an
CC important role in genome stability. {ECO:0000269|PubMed:17189249}.
CC -!- SUBUNIT: Component of the TORC1 complex composed of at least mip1,
CC orb5, tel2, toc1, toc89, tor2, tti1 and wat1. Component of the TORC2
CC complex composed of at least bit61, orb5, sin1, ste20, tel2, tor1, tti1
CC and wat1. Interacts with tti1. {ECO:0000269|PubMed:18076573}.
CC -!- INTERACTION:
CC Q9P3W5; O14356: tor1; NbExp=3; IntAct=EBI-2014377, EBI-2014420;
CC Q9P3W5; Q9Y7K2: tor2; NbExp=3; IntAct=EBI-2014377, EBI-2014299;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the TEL2 family. {ECO:0000305}.
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DR EMBL; AB362536; BAF80889.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB93845.1; -; Genomic_DNA.
DR RefSeq; NP_594697.1; NM_001020125.2.
DR AlphaFoldDB; Q9P3W5; -.
DR BioGRID; 280053; 23.
DR IntAct; Q9P3W5; 9.
DR MINT; Q9P3W5; -.
DR STRING; 4896.SPAC458.03.1; -.
DR iPTMnet; Q9P3W5; -.
DR MaxQB; Q9P3W5; -.
DR PaxDb; Q9P3W5; -.
DR PRIDE; Q9P3W5; -.
DR EnsemblFungi; SPAC458.03.1; SPAC458.03.1:pep; SPAC458.03.
DR GeneID; 2543639; -.
DR KEGG; spo:SPAC458.03; -.
DR PomBase; SPAC458.03; tel2.
DR VEuPathDB; FungiDB:SPAC458.03; -.
DR eggNOG; KOG4346; Eukaryota.
DR HOGENOM; CLU_329325_0_0_1; -.
DR InParanoid; Q9P3W5; -.
DR OMA; AGIMVKL; -.
DR PhylomeDB; Q9P3W5; -.
DR PRO; PR:Q9P3W5; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0070209; C:ASTRA complex; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0110078; C:TTT complex; IDA:PomBase.
DR GO; GO:0051879; F:Hsp90 protein binding; IBA:GO_Central.
DR GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IMP:PomBase.
DR GO; GO:0050821; P:protein stabilization; IBA:GO_Central.
DR GO; GO:0007004; P:telomere maintenance via telomerase; ISO:PomBase.
DR Gene3D; 1.25.40.720; -; 1.
DR InterPro; IPR038528; TEL2_C_sf.
DR InterPro; IPR019337; Telomere_length_regulation_dom.
DR Pfam; PF10193; Telomere_reg-2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..868
FT /note="DNA replication checkpoint protein tel2"
FT /id="PRO_0000353821"
FT REGION 484..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 490
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 868 AA; 99050 MW; 9CCE08BAC91C0798 CRC64;
MKSFASELSK PIQKPRLKEI LKELQHIGVP SPCNLRLYHE LISVIVPTFW NGKINSEIDF
LLAKCFSTLL GLKLLCVRLD NFVQEANLQN IHLVTVYLEL LEKAFECIDL DDNCKLIWES
TVLSDHQKQS LWTEFIFFLG NFKITNSMSA AMLTFKVYDD SKRLKASSMA DYIGVLSSKL
ACIISKPAVK TPEIYSKLFH YLLHSSNLKA FINPLIPLTQ KFCVQLQKLF ADLTVSDQML
FLNQLLLEHN TKYPTNFSYS TARDDRITGS LATLLRLNFS STHFLRLIEF YWGVPTNLII
KRVEVVCSSI SYKESDKKEL EKTVDSLFNI WSNAQFLRSY TLLAQESLTI YLLLFIPKLE
AKYLNNLSKS LMFSNAISCR LDSLDDDIRM HGMIMAEVIS TYSGVSLSNP LAFDVPIMKT
TKAKVLKSLS SLKDEFLPIE ILNDESVIAE TSIEKEETNV THLEKPVISK NDDLIRGDDL
EPYDFPDIDS EDTDDDPTVS RSKTHSPVYV QDLCKMLKDT ESFEKQRVAL ENASKLIKRK
SAFGTELRDH ADELLQTLIS LQNRFDLMNF DEMQMTAIVE LLLTCLDICG PVICTNLFVS
DYSMRQKILI LSCISLAASK FNDDDNERLF PSQLLPGNLH DQFYSPTIEK ISDELERKLV
FPVMSECKDY AEGPKFLQTR YISKQSEIES KKPLPKANRL SLKVDKSLFL LLTSGYMLAS
RKTAFIEPLF LVYYLKTLGI LFFETFTSKI EGANRMLREY VDVLNEVCRL RSDTPEVNEG
LLFSFLAILE ISSGRTLANE FGKHLLFFEA YTKFLFESPD QGEKVKSLSA AVLILFENKL
SEFRTLALEK LLDDSAPLVP ERFGLAGL
