BRAM2_CAEEL
ID BRAM2_CAEEL Reviewed; 1007 AA.
AC G5ECL4;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Neurexin-related protein bam-2 {ECO:0000305};
DE AltName: Full=Branching abnormal protein 2 {ECO:0000312|WormBase:Y71F9AR.1};
DE Flags: Precursor;
GN Name=bam-2 {ECO:0000303|PubMed:14551437, ECO:0000312|WormBase:Y71F9AR.1};
GN ORFNames=Y71F9AR.1 {ECO:0000312|WormBase:Y71F9AR.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAQ76550.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF GLU-513;
RP 639-SER--ASP-1007 AND THR-728.
RX PubMed=14551437; DOI=10.1126/science.1089163;
RA Colavita A., Tessier-Lavigne M.;
RT "A Neurexin-related protein, BAM-2, terminates axonal branches in C.
RT elegans.";
RL Science 302:293-296(2003).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH CASY-1, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF 639-SER--ASP-1007.
RX PubMed=28901288; DOI=10.7554/elife.29257;
RA Kim B., Emmons S.W.;
RT "Multiple conserved cell adhesion protein interactions mediate neural
RT wiring of a sensory circuit in C. elegans.";
RL Elife 6:0-0(2017).
CC -!- FUNCTION: Maintains the normal guidance and termination of axonal
CC branches of VC motorneurons (PubMed:14551437). By associating with cell
CC adhesion protein casy-1, mediates axonal interactions to establish
CC synaptic connections between the male-specific sensory neuron HOA and
CC the AVG interneuron (PubMed:28901288). Plays a role in regulating male
CC mating behavior (PubMed:28901288). {ECO:0000269|PubMed:14551437,
CC ECO:0000269|PubMed:28901288}.
CC -!- SUBUNIT: Interacts with casy-1; the interaction establishes synaptic
CC connections between neurons. {ECO:0000269|PubMed:28901288}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14551437};
CC Single-pass type I membrane protein {ECO:0000255}. Cell projection,
CC axon {ECO:0000269|PubMed:28901288}.
CC -!- TISSUE SPECIFICITY: Expressed in head and tail neurons, the ventral
CC nerve cord, the VulF vulval cell and in the somatic gonad
CC (PubMed:14551437). Expressed in the male-specific sensory neuron HOA
CC (PubMed:28901288). {ECO:0000269|PubMed:14551437,
CC ECO:0000269|PubMed:28901288}.
CC -!- DEVELOPMENTAL STAGE: Expressed from embryogenesis to adulthood.
CC {ECO:0000269|PubMed:14551437}.
CC -!- SIMILARITY: Belongs to the neurexin family. {ECO:0000305}.
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DR EMBL; AY373340; AAQ76550.1; -; mRNA.
DR EMBL; BX284601; CCD73520.1; -; Genomic_DNA.
DR RefSeq; NP_001021809.1; NM_001026638.2.
DR AlphaFoldDB; G5ECL4; -.
DR STRING; 6239.Y71F9AR.1; -.
DR EPD; G5ECL4; -.
DR PaxDb; G5ECL4; -.
DR PeptideAtlas; G5ECL4; -.
DR EnsemblMetazoa; Y71F9AR.1.1; Y71F9AR.1.1; WBGene00000237.
DR GeneID; 171868; -.
DR KEGG; cel:CELE_Y71F9AR.1; -.
DR CTD; 171868; -.
DR WormBase; Y71F9AR.1; CE33243; WBGene00000237; bam-2.
DR eggNOG; ENOG502SMRX; Eukaryota.
DR HOGENOM; CLU_011835_0_0_1; -.
DR InParanoid; G5ECL4; -.
DR OMA; EFNSHRE; -.
DR OrthoDB; 162867at2759; -.
DR PRO; PR:G5ECL4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000237; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:WormBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR SUPFAM; SSF49899; SSF49899; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Glycoprotein; Membrane; Neurogenesis;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..1007
FT /note="Neurexin-related protein bam-2"
FT /id="PRO_5015091919"
FT TOPO_DOM 27..865
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 866..886
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 887..1007
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 980..1007
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 981..995
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 217
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 487
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 617
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 756
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT MUTAGEN 513
FT /note="E->K: In cy4; 66% of animals display defective axon
FT branch formation and guidance in VC motorneurons."
FT /evidence="ECO:0000269|PubMed:14551437"
FT MUTAGEN 639..1007
FT /note="Missing: In cy6; impaired male mating behavior
FT characterized by defective vulva location behavior. 80% of
FT animals display defective axon branch formation and
FT guidance in VC motorneurons. Disrupts axonal connections
FT between the male-specific sensory neuron HOA and the
FT interneuron AVG."
FT /evidence="ECO:0000269|PubMed:14551437,
FT ECO:0000269|PubMed:28901288"
FT MUTAGEN 728
FT /note="T->I: In cy7; 80% of animals display defective axon
FT branch formation and guidance in VC motorneurons."
FT /evidence="ECO:0000269|PubMed:14551437"
SQ SEQUENCE 1007 AA; 113154 MW; 371F8CB2E2DA3F9D CRC64;
MSSERRKDNP LRFALILHLL AVLVQGDIQP RFSCYNHYLA GIRTSSVLPI SGHSSAPSNV
KCIFENDVAF TEIHSALENS YQVMGKKSVN FQVLDLFHLR ELVRRSHCTQ TLTVTWKNVP
EAGKGGLKVV SLLNETTRIE YSGDERQDFL FDETFAGVRH LIPDEDDNES NPTVTTTRLL
CKHIPQPECL VRGKFEVDLE ANLEWSYAFS FKTDITNQTL FTLRCGDTTS EVRLENDFFI
RADGNAPVAV THLSDATWHT AIVKHNQPDS HFLKIDDFPE IELGKSISDD TDKTITLSIS
VNGNIQLIDP TDTNDDCMYS FDKPQKRLQE TVSTRTMCVG CGCPKLSGTF DGLSKCDEED
ENSYNLRRDV DRLSFIHIDN AFDIDSNGAA IPAISTSFKS DSDVGLIFFG YWQNFHGKGR
LQVYYHYDTI SAVFCKHTDD EECSSCSIRS SEGFGKDQWI QTILWGGGDE IHLAVDSSVC
RLQQLSNISL AEVYAIPQIS GGAGLFIGGT WHEKKRRGLY KADAEQKYFE NTREKAPVLR
GCIKDVFVRG SKTDVSEMFE VQKQAMLNEP DDSNAFAVRK YCQSCIPACS EGTRCRSRAP
LQDSAMICDC ADELQFNTSE GTCNRERDTP VALSTAFLSE NQIVLDIQNT KAILSKVWLK
FALPKQINQA QRIVEFNSHR ETLFNINLET DGTVNVQLHG QDTASRQLNL LDDRVHLLQL
QRRTPMGTRH SAKKYDLYID GFHTVVSDIG KLVLNNVSVT AAETEDEWSS VIVHDLGLSY
EYDEHFAISH PSNVIHQVDL HSQLLPYQIR APDLSKTGIL DDSLWEKPAY VTDSEAAPVL
ESSPHGEVVD FTADIIEPEQ LLSGRWILYS LFLTILLCLF ILMCIVCYWC VLRPRAMRRT
DTGSQRTIIR DSPDYAPVKM RRDSIGGISI DDDGSIGTDD TDLQAYRDIP SHRVKIYRES
MVSILVPSID QPAEAAIVKR SHSTISDQTP SRPHDTSHAP LVAVNDD
