TENAE_SOYBN
ID TENAE_SOYBN Reviewed; 229 AA.
AC Q9SWB6;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Probable bifunctional TENA-E protein {ECO:0000250|UniProtKB:Q9ASY9};
DE EC=3.5.1.- {ECO:0000250|UniProtKB:Q9ASY9};
DE EC=3.5.99.2 {ECO:0000250|UniProtKB:Q9ASY9};
DE AltName: Full=Aminopyrimidine aminohydrolase {ECO:0000250|UniProtKB:Q9ASY9};
DE AltName: Full=Formylaminopyrimidine amidohydrolase {ECO:0000250|UniProtKB:Q9ASY9};
DE AltName: Full=Formylaminopyrimidine deformylase {ECO:0000250|UniProtKB:Q9ASY9};
DE AltName: Full=Seed maturation protein PM36 {ECO:0000303|Ref.1};
GN Name=TENA_E {ECO:0000250|UniProtKB:Q9ASY9};
GN Synonyms=PM36 {ECO:0000303|Ref.1}; OrderedLocusNames=Glyma17g03600;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Chow T.Y., Lin T.Y., Lin T.Y., Liu S.M., Hsing Y.I.C.;
RT "Characterization of a soybean seed maturation protein, PM36.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
CC -!- FUNCTION: May be involved in thiamine salvage.
CC {ECO:0000250|UniProtKB:Q9ASY9}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-
CC hydroxymethyl-2-methylpyrimidine + NH4(+); Xref=Rhea:RHEA:31799,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:16892, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:63416; EC=3.5.99.2; Evidence={ECO:0000305};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine = 4-
CC amino-5-aminomethyl-2-methylpyrimidine + formate;
CC Xref=Rhea:RHEA:46212, ChEBI:CHEBI:15377, ChEBI:CHEBI:15740,
CC ChEBI:CHEBI:63416, ChEBI:CHEBI:85895; Evidence={ECO:0000305};
CC -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the thiaminase-2 family. {ECO:0000305}.
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DR EMBL; AF169021; AAD51624.1; -; mRNA.
DR RefSeq; NP_001238329.1; NM_001251400.1.
DR AlphaFoldDB; Q9SWB6; -.
DR SMR; Q9SWB6; -.
DR STRING; 3847.GLYMA17G03600.1; -.
DR PRIDE; Q9SWB6; -.
DR EnsemblPlants; KRH02337; KRH02337; GLYMA_17G032700.
DR GeneID; 547557; -.
DR Gramene; KRH02337; KRH02337; GLYMA_17G032700.
DR KEGG; gmx:547557; -.
DR eggNOG; ENOG502QQ9D; Eukaryota.
DR HOGENOM; CLU_055855_1_0_1; -.
DR InParanoid; Q9SWB6; -.
DR OMA; FNTWLVQ; -.
DR OrthoDB; 1133952at2759; -.
DR UniPathway; UPA00060; -.
DR Proteomes; UP000008827; Chromosome 17.
DR Genevisible; Q9SWB6; GM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0050334; F:thiaminase activity; IEA:UniProtKB-EC.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.20.910.10; -; 1.
DR InterPro; IPR016084; Haem_Oase-like_multi-hlx.
DR InterPro; IPR004305; Thiaminase-2/PQQC.
DR Pfam; PF03070; TENA_THI-4; 1.
DR SUPFAM; SSF48613; SSF48613; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Hydrolase; Reference proteome; Thiamine biosynthesis.
FT CHAIN 1..229
FT /note="Probable bifunctional TENA-E protein"
FT /id="PRO_0000192045"
FT DISULFID 158..173
FT /evidence="ECO:0000250|UniProtKB:Q9ASY9"
SQ SEQUENCE 229 AA; 25988 MW; D251A569A495853E CRC64;
MEEKAKAEQK KIGMTETWLK KHRLLYNGAT RHPLIISIRD GTINTASFKT WLAQDYLFVR
AFVPFVASVL IKAWKESDCS GDMEVILGGM ASLEDEISWF KTEANKWGIS LSDVVPQQAN
KNYCGLLESL MSPDAEYTVA ITAFWAIETV YQESFAHCIE EGSKTPPELK ETCVRWGNEA
FGKYCQSLQN IANRCLQKAS DEELKKAEVM LLSVLEHEVE FWNMSRGNV
