TEP1_RAT
ID TEP1_RAT Reviewed; 2629 AA.
AC O08653;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Telomerase protein component 1;
DE Short=rTLP1;
DE AltName: Full=Telomerase-associated protein 1;
DE Short=Telomerase protein 1;
DE AltName: Full=p230;
DE AltName: Full=p240;
DE AltName: Full=p80 telomerase homolog;
GN Name=Tep1; Synonyms=Tlp1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION IN THE TELOMERASE
RP RIBONUCLEOPROTEIN COMPLEX.
RC STRAIN=Fischer 344; TISSUE=Fibroblast;
RX PubMed=9118230; DOI=10.1016/s0092-8674(00)81933-9;
RA Nakayama J., Saito M., Nakamura H., Matsuura A., Ishikawa F.;
RT "TLP1: a gene encoding a protein component of mammalian telomerase is a
RT novel member of WD repeats family.";
RL Cell 88:875-884(1997).
RN [2]
RP IDENTIFICATION IN THE VAULTS RIBONUCLEOPROTEIN PARTICLE.
RX PubMed=10551828; DOI=10.1074/jbc.274.46.32712;
RA Kickhoefer V.A., Stephen A.G., Harrington L., Robinson M.O., Rome L.H.;
RT "Vaults and telomerase share a common subunit, TEP1.";
RL J. Biol. Chem. 274:32712-32717(1999).
RN [3]
RP ELECTRON CRYOMICROSCOPY (22 ANGSTROMS) OF VAULT COMPLEX AND TEP1 WD REPEATS
RP MODELING.
RX PubMed=10864046; DOI=10.1017/s1355838200000157;
RA Kong L.B., Siva A.C., Kickhoefer V.A., Rome L.H., Stewart P.L.;
RT "RNA location and modeling of a WD40 repeat domain within the vault.";
RL RNA 6:890-900(2000).
CC -!- FUNCTION: Component of the telomerase ribonucleoprotein complex that is
CC essential for the replication of chromosome termini (By similarity).
CC Also a component of the ribonucleoprotein vaults particle, a multi-
CC subunit structure involved in nucleo-cytoplasmic transport. Responsible
CC for the localizing and stabilizing vault RNA (vRNA) association in the
CC vault ribonucleoprotein particle. Binds to TERC (By similarity).
CC {ECO:0000250|UniProtKB:P97499, ECO:0000250|UniProtKB:Q99973}.
CC -!- SUBUNIT: Associated component of the telomerase holoenzyme complex (By
CC similarity). Component of the vault ribonucleoprotein particle, at
CC least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds
CC to VAULTRC1, VAULTRC2 and VAULTRC4/hvg4 vRNAs (By similarity).
CC {ECO:0000250|UniProtKB:P97499, ECO:0000250|UniProtKB:Q99973}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
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DR EMBL; U89282; AAB51690.1; -; mRNA.
DR PIR; T32735; T32735.
DR RefSeq; NP_072113.1; NM_022591.2.
DR AlphaFoldDB; O08653; -.
DR STRING; 10116.ENSRNOP00000012235; -.
DR CarbonylDB; O08653; -.
DR iPTMnet; O08653; -.
DR PhosphoSitePlus; O08653; -.
DR PaxDb; O08653; -.
DR PRIDE; O08653; -.
DR GeneID; 64523; -.
DR KEGG; rno:64523; -.
DR UCSC; RGD:3869; rat.
DR CTD; 7011; -.
DR RGD; 3869; Tep1.
DR eggNOG; KOG3602; Eukaryota.
DR eggNOG; KOG4155; Eukaryota.
DR InParanoid; O08653; -.
DR OrthoDB; 29963at2759; -.
DR PhylomeDB; O08653; -.
DR PRO; PR:O08653; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0016363; C:nuclear matrix; ISO:RGD.
DR GO; GO:1990904; C:ribonucleoprotein complex; IDA:RGD.
DR GO; GO:0005697; C:telomerase holoenzyme complex; IDA:BHF-UCL.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0019899; F:enzyme binding; ISO:RGD.
DR GO; GO:0002039; F:p53 binding; ISO:RGD.
DR GO; GO:0003723; F:RNA binding; ISO:RGD.
DR GO; GO:0070034; F:telomerase RNA binding; ISO:RGD.
DR GO; GO:0006278; P:RNA-templated DNA biosynthetic process; IC:BHF-UCL.
DR GO; GO:0000722; P:telomere maintenance via recombination; ISO:RGD.
DR Gene3D; 2.130.10.10; -; 6.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR025139; DUF4062.
DR InterPro; IPR045804; DUF5920.
DR InterPro; IPR007111; NACHT_NTPase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008850; TEP1_N.
DR InterPro; IPR008858; TROVE_dom.
DR InterPro; IPR037214; TROVE_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF13271; DUF4062; 1.
DR Pfam; PF19334; DUF5920; 1.
DR Pfam; PF05729; NACHT; 1.
DR Pfam; PF05386; TEP1_N; 4.
DR Pfam; PF05731; TROVE; 1.
DR Pfam; PF00400; WD40; 5.
DR SMART; SM00320; WD40; 16.
DR SUPFAM; SSF140864; SSF140864; 1.
DR SUPFAM; SSF50978; SSF50978; 4.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50837; NACHT; 1.
DR PROSITE; PS51226; TEP1_N; 4.
DR PROSITE; PS50988; TROVE; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW ATP-binding; Chromosome; Nucleotide-binding; Nucleus; Reference proteome;
KW Repeat; Ribonucleoprotein; RNA-binding; Telomere; WD repeat.
FT CHAIN 1..2629
FT /note="Telomerase protein component 1"
FT /id="PRO_0000050984"
FT REPEAT 1..30
FT /note="TEP1 N-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00575,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 31..60
FT /note="TEP1 N-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00575,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 61..90
FT /note="TEP1 N-terminal 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00575,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 91..120
FT /note="TEP1 N-terminal 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00575,
FT ECO:0000269|PubMed:10864046"
FT DOMAIN 231..689
FT /note="TROVE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00343"
FT DOMAIN 1175..1582
FT /note="NACHT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT REPEAT 1424..1461
FT /note="WD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1685..1724
FT /note="WD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1727..1765
FT /note="WD 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1768..1807
FT /note="WD 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1809..1848
FT /note="WD 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1851..1890
FT /note="WD 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1893..1934
FT /note="WD 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1936..1975
FT /note="WD 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 1978..2016
FT /note="WD 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2019..2058
FT /note="WD 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2070..2109
FT /note="WD 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2116..2154
FT /note="WD 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2157..2194
FT /note="WD 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2200..2244
FT /note="WD 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2247..2285
FT /note="WD 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2288..2327
FT /note="WD 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2329..2365
FT /note="WD 17"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2378..2427
FT /note="WD 18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2470..2510
FT /note="WD 19"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2555..2592
FT /note="WD 20"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REPEAT 2594..2628
FT /note="WD 21"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:10864046"
FT REGION 390..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1181..1188
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
SQ SEQUENCE 2629 AA; 291708 MW; B90C3F72E5D4B6BE CRC64;
MEKLCGYVPV HPDILSLKNR CLTMLSDIQP LEKIHGQRSV NPDILSLENR CLTLLPDLQP
MEKIHGQRSV HPDILSSENR CLTLLPDLQS LEKLCGHMSS HPDVLSLENR CLATLPTVKR
TVSSGPLLQC LHRSHTAQAD LRDPNFRNCL FPEPPTIEAP CFLKELDLPT GPRALKSMSA
TARVQEVALG QRCVSEGKEL QEEKESAEVP MPLYSLSLGG EEEEVVGAPV LKLTSGDSDS
HPETTDQILQ EKKMALLTLL CSAMASSVNV KDASDPTRAS IHEVCSALAP LEPEFILKAS
LYARQQLNLR DIANIVLAVA ALLPACRPHV RRYYSAIVHL PSDWIQVAEF YQSLAEGDEK
KLVPLPACLR AAMTDKFAQF DEYQLAKYNP RKHRSKTRSR QPPRPQRTKP PFSESGKCFP
KSVWPLKNEQ ISFEAAYNAV SEKKRLPRFT LKKLVEQLHI HEPAQHVQAL LGYRYPSTLE
LFSRSHLPGP WDSSRAGQRM KLQRPETWER ELSLRGNRAS VWEELIDNGK LPFMAMLRNL
CNLLRTGISA HHHELVLQRL QHEKSVIHSR QFPFRFLNAH DSLDRLEAQL RSKASPFPSN
TTLMKRIMIR NSKKIKRPAN PRYLCTLTQR QLRAAMAIPV MYEHLKREKL RLHKARQWTC
DLELLERYRQ ALETAVNISV KHNLPPLPGR TLLVYLTDAN ANRLCPKSHL QGPPLNYVLL
LIGMMMARAE QTTVWLCGTG TVKTPVLTAD EGILKTAIKL QAQVQELEEN DEWPLETFEK
YLLSLAVRRT PIDRVILFGQ RMDTELLNVA KQIIWQHVNS KCLFVSVLLR KMQYMSPNLN
PNDVTLSGCT DGILKFIAEH GASRLLEHVG QLDKIFKIPP PPGKTKVSPL RPLEENNPGP
FVPISQHGWR NIRLFISSTF RDMHGERDLL MRSVLPALQA RAFPHRISLH AIDLRWGITE
EETRRNRQLE VCLGEVENSQ LFVGILGSRY GYTPPSYDLP DHPHFHWTQR YPSGRSVTEM
EVMQFLNRGQ RSEPSDQALI YFRDPGFLSS VPDVWKPDFI SESEEAAHRV SELKRFLQEQ
KEVTCRRYSC EWGGVAAGRP YTGGLEEFGQ LVLQDVWSVI QKRYLQPGAQ LEQPGSISEE
DLIQASFQQL KSPPSPARPR LLQDTVQQLM LPHGRLSLVI GQAGQGKTAF LASLVSALKV
PDQPNVAPFV FFHFSAARPD QCLAFNLLRR LCTHLHQKLG EPSALPSTYR GLVWELQQKL
LLKSAQWLQP GQTLVLIIDG ADKLVDHNGQ LISDWIPKSL PRRVHLVLSV SSDSGLGETL
QQSQSAYVVA LGSLVPSSRA QLVREELALY GKRLEESPFN NQMRLLLAKQ GSSLPLYLHL
VTDYLRLFTL YEQVSERLRT LPATLPLLLQ HILSTLEQEH GHNVLPQALT ALEVTHSGLT
VDQLHAVLST WLTLPKETKS WEEAVAASHS GNLYPLAPFA YLVQSLRSLL GEGPVERPGA
RLCLSDGPLR TAVKRRYGKR LGLEKTAHVL IAAHLWKMCD PDASGTFRSC PPEALKDLPY
HLLQSGNHGL LAKFLTNLHV VAAYLEVGLV PDLLEAYELY ASSKPEVNQK LPEADVAVFH
NFLKQQASLL TQYPLLLLQQ AASQPEESPV CCQAPLLTQR WHNQCILKWI NKPQTLKGQQ
SLSLPISSSP TAVAFSPNGQ RAAVGTAGGT IYLLNLRTWQ EEKALVSGCD GISSFAFLSD
TALFLTTFDG LLELWDLQHG CWVFQTKAHQ YQITGCCLSP DRRLLATVCL GGYVKLWDTV
QGQLAFQYTH PKSLNCITFH PEGQVVATGN WSGIVTFFQA DGLKVTKELG GPGPSVRTLA
FSAPGKVVAL GRIDGTVELW AWQEGTRLAA FPAQCGGVST VLFLHAGGRF LTAGEDGKAQ
LWSGFLGRPR GCLGSLYLSP ALSVALNPDG DQVAVGYRGD GIKIYRISSG PQEAQCQELN
VAVSALVWLS PSVLVSGAED GSLHGWMLRR NSLQSLWLSS VCQKPVLGLA ASQEFLASAS
EDFTVRLWPR QLLTQPHAVE ELPCAAELRG HEGPVCCCSF SPDGRILATA GRDRNLLCWD
VKVAQAPLLI HTFSSCHRDW ITGCTWTKDN ILISCSSDGS VGLWNPEAGQ QLGQFPGHQS
AVSAVVAVEE HIVSVSRDGT LKVWDRQGVE LTSIPAHSGP ISQCAAALEP RPAGQPGSEL
MVVTVGLDGA TKLWHPLLVC QIHTLQGHSG PVTAAAASEA SGLLLTSDNS SVRLWQIPKE
ADDTCKPRSS AVITAVAWAP DGSLVVSGNE AGELTLWQKA QAVATARAPG RVSDLIWCSA
NAFFVLSANE NVSEWQVELR KGSTCTNFRL YLKRVLQEDL GVLTGMALAP DGQSLILMKE
DVELLQMKPG STPSSICRRY AVHSSILCTS KDYGLFYLQQ GNSGSLSILE QEESGKFEKT
LDFNLNLNNP NGSPVSITQA EPESGSSLLC ATSDGMLWNL SECTPEGEWV VDNIWQKKSR
NPKSRTPGTD SSPGLFCMDS WVEPTHLKAR QCKKIHLGSV TALHVLPGLL VTASEDRDVK
LWERPSMQLL GLFRCEGPVS CLEPWMEPSS PLQLAVGDAQ GNLYFLSWE
