TEPA_BACSU
ID TEPA_BACSU Reviewed; 245 AA.
AC Q99171;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 4.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Translocation-enhancing protein TepA;
GN Name=tepA; Synonyms=ylxI, ymfB; OrderedLocusNames=BSU16790;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP SEQUENCE REVISION TO 142 AND 144.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-245.
RC STRAIN=168;
RX PubMed=3129532; DOI=10.1099/00221287-133-9-2359;
RA Butler P.D., Mandelstam J.;
RT "Nucleotide sequence of the sporulation operon, spoIIIE, of Bacillus
RT subtilis.";
RL J. Gen. Microbiol. 133:2359-2370(1987).
RN [4]
RP FUNCTION.
RC STRAIN=168;
RX PubMed=10455123; DOI=10.1074/jbc.274.35.24585;
RA Bolhuis A., Matzen A., Hyyrylaeinen H.-L., Kontinen V.P., Meima R.,
RA Chapuis J., Venema G., Bron S., Freudl R., van Dijl J.M.;
RT "Signal peptide peptidase- and ClpP-like proteins of Bacillus subtilis
RT required for efficient translocation and processing of secretory
RT proteins.";
RL J. Biol. Chem. 274:24585-24592(1999).
CC -!- FUNCTION: Required for efficient translocation of pre-proteins across
CC the membrane. {ECO:0000269|PubMed:10455123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase S14 family. TepA subfamily.
CC {ECO:0000305}.
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DR EMBL; AL009126; CAB13552.3; -; Genomic_DNA.
DR EMBL; M17445; AAA22783.1; -; mRNA.
DR PIR; A69885; A69885.
DR RefSeq; NP_389561.3; NC_000964.3.
DR RefSeq; WP_003245712.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; Q99171; -.
DR SMR; Q99171; -.
DR STRING; 224308.BSU16790; -.
DR MEROPS; S14.012; -.
DR PaxDb; Q99171; -.
DR PRIDE; Q99171; -.
DR EnsemblBacteria; CAB13552; CAB13552; BSU_16790.
DR GeneID; 936370; -.
DR KEGG; bsu:BSU16790; -.
DR PATRIC; fig|224308.179.peg.1821; -.
DR eggNOG; COG0740; Bacteria.
DR OMA; KMQDRVI; -.
DR PhylomeDB; Q99171; -.
DR BioCyc; BSUB:BSU16790-MON; -.
DR BRENDA; 3.4.24.78; 658.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR023562; ClpP/TepA.
DR Pfam; PF00574; CLP_protease; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Hydrolase; Protease; Protein transport; Reference proteome;
KW Translocation; Transport.
FT CHAIN 1..245
FT /note="Translocation-enhancing protein TepA"
FT /id="PRO_0000179764"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 124
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 147
FT /evidence="ECO:0000250"
FT CONFLICT 142..144
FT /note="ATM -> STV (in Ref. 3; AAA22783)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 26918 MW; EFE3663A9D7C7244 CRC64;
MDHRMENTEE ERPEKNDAKD SIMNKIQQLG ETTLPQLPQD TNIHCLTIIG QIEGHVQLPP
QNKTTKYEHV IPQIVAIEQN PKIEGLLIIL NTVGGDVEAG LAIAEMLASL SKPTVSIVLG
GGHSIGVPIA VSCDYSYIAE TATMTIHPVR LTGLVIGVPQ TFEYLDKMQE RVVKFVTSHS
NITEEKFKEL MFSKGNLTRD IGTNVVGKDA VKYGLIDHAG GVGQAINKLN ELIDEARKEE
GRMIQ
