ID   TERL_BPMU               Reviewed;         551 AA.
AC   Q9T1W6;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   23-FEB-2022, entry version 70.
DE   RecName: Full=Probable terminase, large subunit gp28;
DE            EC=3.1.-.- {ECO:0000305};
DE   AltName: Full=Gene product 28;
DE            Short=gp28;
DE   AltName: Full=Gene product E;
DE            Short=gpE;
DE   AltName: Full=Pacase, large subunit;
DE   AltName: Full=Packaging protein E;
GN   OrderedLocusNames=Mup28;
OS   Escherichia phage Mu (Bacteriophage Mu).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Muvirus.
OX   NCBI_TaxID=10677;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11922669; DOI=10.1006/jmbi.2002.5437;
RA   Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.;
RT   "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like
RT   prophages in Haemophilus, Neisseria and Deinococcus.";
RL   J. Mol. Biol. 317:337-359(2002).
RN   [2]
RP   INDUCTION.
RX   PubMed=8293968; DOI=10.1093/genetics/135.3.619;
RA   Chiang L.W., Howe M.M.;
RT   "Mutational analysis of a C-dependent late promoter of bacteriophage Mu.";
RL   Genetics 135:619-629(1993).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=14745593; DOI=10.1007/s00705-003-0216-4;
RA   Siboo I.R., Sieder F., Kumar K., Howe M.M., DuBow M.S.;
RT   "Characterization of Plys-proximal morphogenetic genes of transposable
RT   bacteriophage Mu.";
RL   Arch. Virol. 149:241-259(2004).
CC   -!- FUNCTION: The terminase large subunit acts as an ATP driven molecular
CC       motor necessary for viral DNA translocation into empty capsids and as
CC       an endonuclease that cuts the viral genome to initiate and to end a
CC       packaging reaction. The terminase lies at a unique vertex of the
CC       procapsid and is composed of two subunits, a small terminase subunit
CC       involved in viral DNA recognition (packaging sequence), and a large
CC       terminase subunit possessing endonucleolytic and ATPase activities.
CC       Both terminase subunits heterooligomerize and are docked on the portal
CC       protein to form the packaging machine. The terminase large subunit
CC       exhibits endonuclease activity and cleaves the viral genome concatemer
CC       once the capsid is full (headful packaging). Once the capsid is
CC       packaged with the DNA, the terminase complex is substituted by neck
CC       proteins. {ECO:0000250|UniProtKB:P17312}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:P17312};
CC       Note=Binds 2 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:P17312};
CC   -!- SUBUNIT: Interacts with the terminase small subunit gp28.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       Expression of late genes is activated by the viral late transcription
CC       activator C. {ECO:0000269|PubMed:8293968}.
CC   -!- SIMILARITY: Belongs to the T4likevirus large terminase family.
CC       {ECO:0000305}.
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DR   EMBL; AF083977; AAF01106.1; -; Genomic_DNA.
DR   RefSeq; NP_050632.1; NC_000929.1.
DR   GeneID; 2636303; -.
DR   KEGG; vg:2636303; -.
DR   Proteomes; UP000002611; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0032359; P:provirus excision; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR012036; Phage_Mu_Gp28.
DR   PIRSF; PIRSF007056; UCP007056; 1.
PE   2: Evidence at transcript level;
KW   Endonuclease; Host cytoplasm; Hydrolase; Late protein; Magnesium;
KW   Metal-binding; Nuclease; Reference proteome; Viral genome excision;
KW   Viral genome packaging; Viral release from host cell.
FT   CHAIN           1..551
FT                   /note="Probable terminase, large subunit gp28"
FT                   /id="PRO_0000077822"
SQ   SEQUENCE   551 AA;  62636 MW;  B63D646144653A7C CRC64;
     MNTRENNLKA LHAPRKINLR EEAGLLGVDI VTDIGEAQPR NEPVFLGYQR RWFEDESQIC
     IAEKSRRTGL TWAEAGRNVM TAAKPKRRGG RNVFYVGSRQ EMALEYIAAC ALFARAFNQL
     AKADVWEQTF WDSDKKEEIL TYMIRFPNSG FKIQALSSRP SNLRGLQGDV VIDEAAFHEA
     LDELLKAAFA LNMWGASVRI ISTHNGVDNL FNQYIQDARE GRKDYSVHRI TLDDAIADGL
     YRRICYVTNQ PWSPEAEKAW RDGLYRNAPN KESADEEYGC IPKKSGGAYL SRVLIEAAMT
     PARDIPVLRF EAPDDFESLT PQMRHGIVQD WCEQELLPLL DALSPLNKHV LGEDFARRGD
     LTVFVPLAIT PDLRKRECFR VELRNVTYDQ QRQILLFILS RLPRFTGAAF DATGNGGYLA
     EAARLIYGPE MIDCISLTPA WYQEWMPKLK GEFEAQNITI ARHQTTLDDL LHIKVDKGIP
     QIDKGRTKDE GGKGRRHGDF AVALCMAVRA SYMNGFVIDE DSIQALPPRH RGDDVDNDDF
     DDYHQFERGG W