BRC1_DROME
ID BRC1_DROME Reviewed; 727 AA.
AC Q01295; O46065; O46066; Q01293; Q01296; Q9W571; Q9W574;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 2.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Broad-complex core protein isoforms 1/2/3/4/5;
GN Name=br; Synonyms=Br-C; ORFNames=CG11491;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Oregon-R; TISSUE=Larva;
RX PubMed=1743483; DOI=10.1093/genetics/129.2.385;
RA DiBello P.R., Withers D.A., Bayer C.A., Fristrom J.W., Guild G.M.;
RT "The Drosophila Broad-Complex encodes a family of related proteins
RT containing zinc fingers.";
RL Genetics 129:385-397(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 4 AND 5).
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5]
RP DEVELOPMENTAL STAGE, AND CHARACTERIZATION OF ISOFORMS.
RC TISSUE=Imaginal disk, and Larva;
RX PubMed=8660872; DOI=10.1006/dbio.1996.0140;
RA Bayer C.A., Holley B., Fristrom J.W.;
RT "A switch in broad-complex zinc-finger isoform expression is regulated
RT posttranscriptionally during the metamorphosis of Drosophila imaginal
RT discs.";
RL Dev. Biol. 177:1-14(1996).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=8223281; DOI=10.1242/dev.118.2.613;
RA Huet F., Ruiz C., Richards G.;
RT "Puffs and PCR: the in vivo dynamics of early gene expression during
RT ecdysone responses in Drosophila.";
RL Development 118:613-627(1993).
RN [7]
RP CHARACTERIZATION OF ISOFORMS, AND MUTAGENESIS.
RX PubMed=9242423; DOI=10.1006/dbio.1997.8620;
RA Bayer C.A., von Kalm L., Fristrom J.W.;
RT "Relationships between protein isoforms and genetic functions demonstrate
RT functional redundancy at the Broad-Complex during Drosophila
RT metamorphosis.";
RL Dev. Biol. 187:267-282(1997).
CC -!- FUNCTION: Broad-complex proteins are required for puffing and
CC transcription of salivary gland late genes during metamorphosis.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=1; Synonyms=BRCORE-TNT1-Q1-Z1;
CC IsoId=Q01295-1; Sequence=Displayed;
CC Name=2; Synonyms=BRCORE-Q1-Z1;
CC IsoId=Q01295-2; Sequence=VSP_006836;
CC Name=3; Synonyms=BRCORE-Q2-Z1;
CC IsoId=Q01295-3; Sequence=VSP_006835;
CC Name=4; Synonyms=BRCORE-Z2;
CC IsoId=Q01295-4; Sequence=VSP_006837, VSP_006838;
CC Name=5; Synonyms=BRCORE-NS-Z3;
CC IsoId=Q01295-5; Sequence=VSP_006839;
CC Name=6; Synonyms=BRCORE-Z4;
CC IsoId=Q24206-1; Sequence=External;
CC -!- DEVELOPMENTAL STAGE: Isoforms Z1 accumulate slowly in mid instar larvae
CC salivary glands at beginning of ecdysone response (94-114 hours of
CC development at puff stage 1) and become the predominant isoform after 6
CC hours. Levels diminish at puff stage 2 and are moderately abundant in
CC late larvae from stages 3-10. In prepupae, transcripts appear at puff
CC stage 15 onwards, reaching maximum at stages 18-20. In gut, levels
CC remain constant between stages 1-11. In Malpighian tubules, Z1 isoforms
CC are seen at stages 3 and 7, but not at stage 11. In fat body and wing
CC disks, low levels increase between stages 3 and 11. Isoform Z2
CC accumulates to a high level at the beginning of the ecdysone response
CC during puff stage 1 and abruptly disappears after several hours. In
CC prepupae, transcripts are reinduced at low levels. Low levels are seen
CC in the Malpighian Tubules, gut and fat body between stages 1-11 and
CC high levels in the wing disk. Isoform Z3; in mid instar larval salivary
CC gland transcript accumulates to a high level at the beginning of the
CC ecdysone response, 94-98 hours of development in puff stage 1, and
CC abruptly disappears after several hours. Levels increase by puff stage
CC 3 remaining abundant in late larvae until stage 10, then diminish by
CC stage 11. In prepupae, transcripts are abundant and increase during
CC puff stages 11-14 and 18-20. High levels are seen in Malpighian
CC tubules, gut and fat body between stages 1-11 and low levels in wing
CC disk. {ECO:0000269|PubMed:8223281, ECO:0000269|PubMed:8660872}.
CC -!- INDUCTION: Primary response to 20-hydroxyecdysone in third instar
CC larval imaginal disks.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA15629.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X54666; CAA38477.1; -; mRNA.
DR EMBL; X54665; CAA38476.1; -; mRNA.
DR EMBL; X54663; CAA38474.1; -; mRNA.
DR EMBL; X54664; CAA38475.1; -; mRNA.
DR EMBL; AE014298; AAF45648.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45651.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09054.1; -; Genomic_DNA.
DR EMBL; AL009146; CAA15629.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL009146; CAA15626.1; -; Genomic_DNA.
DR EMBL; AL009146; CAA15628.1; -; Genomic_DNA.
DR PIR; S21912; S21912.
DR PIR; S21913; S21913.
DR PIR; S21914; S21914.
DR RefSeq; NP_001188525.1; NM_001201596.3. [Q01295-4]
DR RefSeq; NP_001188526.1; NM_001201597.1.
DR RefSeq; NP_001188527.1; NM_001201598.1.
DR RefSeq; NP_001245462.1; NM_001258533.1. [Q01295-2]
DR RefSeq; NP_524759.2; NM_080020.4.
DR RefSeq; NP_726749.2; NM_166893.2. [Q01295-4]
DR RefSeq; NP_726750.1; NM_166894.4. [Q01295-5]
DR RefSeq; NP_726754.1; NM_166898.2. [Q01295-2]
DR AlphaFoldDB; Q01295; -.
DR SMR; Q01295; -.
DR BioGRID; 69091; 41.
DR IntAct; Q01295; 12.
DR DNASU; 44505; -.
DR EnsemblMetazoa; FBtr0070265; FBpp0070255; FBgn0283451. [Q01295-5]
DR EnsemblMetazoa; FBtr0070266; FBpp0070256; FBgn0283451. [Q01295-2]
DR EnsemblMetazoa; FBtr0300429; FBpp0289658; FBgn0283451. [Q01295-4]
DR EnsemblMetazoa; FBtr0303562; FBpp0292595; FBgn0283451. [Q01295-4]
DR EnsemblMetazoa; FBtr0308319; FBpp0300638; FBgn0283451. [Q01295-2]
DR GeneID; 44505; -.
DR CTD; 103946; -.
DR FlyBase; FBgn0283451; br.
DR VEuPathDB; VectorBase:FBgn0283451; -.
DR OMA; TYHKTRP; -.
DR SignaLink; Q01295; -.
DR BioGRID-ORCS; 44505; 1 hit in 1 CRISPR screen.
DR ChiTaRS; br; fly.
DR GenomeRNAi; 44505; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0283451; Expressed in wing disc and 23 other tissues.
DR ExpressionAtlas; Q01295; baseline and differential.
DR Genevisible; Q01295; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0006914; P:autophagy; IMP:FlyBase.
DR GO; GO:0071390; P:cellular response to ecdysone; IMP:FlyBase.
DR GO; GO:0001752; P:compound eye photoreceptor fate commitment; IMP:FlyBase.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0007562; P:eclosion; IEP:FlyBase.
DR GO; GO:0042332; P:gravitaxis; IMP:FlyBase.
DR GO; GO:0048808; P:male genitalia morphogenesis; IMP:FlyBase.
DR GO; GO:0055001; P:muscle cell development; IGI:FlyBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0007458; P:progression of morphogenetic furrow involved in compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0040034; P:regulation of development, heterochronic; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035075; P:response to ecdysone; IMP:FlyBase.
DR GO; GO:0009608; P:response to symbiont; IMP:FlyBase.
DR GO; GO:0035070; P:salivary gland histolysis; IMP:FlyBase.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..727
FT /note="Broad-complex core protein isoforms 1/2/3/4/5"
FT /id="PRO_0000047069"
FT DOMAIN 32..97
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 636..659
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 666..689
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 135..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 218..378
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 445..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 604..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 694..727
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..267
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..344
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..366
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 703..727
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 432..495
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_006836"
FT VAR_SEQ 432..478
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_006835"
FT VAR_SEQ 433..727
FT /note="KLTTNATTTTTTINNSITNNNNNNNNNNYDYSLPTKNSNSQKTPSPTTTTLT
FT TPTTTTPTRPTAITSASGICGLNLSTFAANGSSSGGSNGGLSMTALLPQQQQQQQQQHQ
FT MSQQQQQQQQQQQQQGNSSSGQQQQPNGILACSTPKANTPTTTQQQMYAAVMAAAASAS
FT ASTSGSANSSLNNSNSTLNTSGGLNNSASGGDDFRCNPCNKNLSSLTRLKRHIQNVHMR
FT PTKEPVCNICKRVYSSLNSLRNHKSIYHRNLKQPKQEPGVGATQAAANSFYHQQHQQQQ
FT LNHHSSS -> PHSITRSAATSPTSSTSSPPSPPTALISPTSSLKGSLAAAVYSLHSHA
FT HGHVLGHATSPPRPGSVGSSVGSNLCTSTSMGCGVNSGNNSGNNNGNNANNNSNNGNAT
FT NNNNNSSSSSTSPGSQATAAGGTVTQAGTPPLPLRMPPPTSGGINEPQECPYCRRTFSC
FT YYSLKRHFQDKHEQSDTLYVCEFCHRRYRTKNSLTTHKSLQHRGSSGMLKRLLKTTAIK
FT HGLVGHGHGHGHVHHPHAHHHALSHPRTSLYDFTSELGQPPPGIQ (in isoform
FT 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_006839"
FT VAR_SEQ 433..514
FT /note="KLTTNATTTTTTINNSITNNNNNNNNNNYDYSLPTKNSNSQKTPSPTTTTLT
FT TPTTTTPTRPTAITSASGICGLNLSTFAAN -> NSPKKLFSCQLCGKLLCSKASLKRH
FT IADKHAVRQEEYRCAICERVYCSRNSLMTHIYTYHKSRPGEMEMKDIKLYNQFNSSI
FT (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_006837"
FT VAR_SEQ 515..727
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_006838"
FT CONFLICT 230
FT /note="V -> L (in Ref. 1; CAA38477/CAA38474/CAA38475)"
FT /evidence="ECO:0000305"
FT CONFLICT 457..459
FT /note="Missing (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 460
FT /note="N -> NN (in Ref. 1; CAA38477)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 727 AA; 77393 MW; 97BBA20F3BD734D6 CRC64;
MDDTQHFCLR WNNYQSSITS AFENLRDDEA FVDVTLACEG RSIKAHRVVL SACSPYFREL
LKSTPCKHPV ILLQDVNFMD LHALVEFIYH GEVNVHQKSL QSFLKTAEVL RVSGLTQQQA
EDTHSHLAQI QNLANSGGRT PLNTHTQSLP HPHHGSLHDD GGSSTLFSRQ GAGSPPPTAV
PSLPSHINNQ LLKRMAMMHR SSAAAAAEET SHAFKRLRGS DNSLPLSGAV GSGSNNNSPD
LPPLHARSAS PQQTPADFST IKHHNNNNTP PLKEEKRNGP TGNGNSGNGN GNGNGASNGN
GISISDKLGS LTPSPLARAG ADDVKSEPMD MVCSNNNANA NDEHSNDSTG EHDANRSSSG
DGGKGSLSSG NDEEIGDGLA SHHAAPQFIM SPAENKMFHA AAFNFPNIDP SALLGLNTQL
QQSGDLAVSP QGKLTTNATT TTTTINNSIT NNNNNNNNNN YDYSLPTKNS NSQKTPSPTT
TTLTTPTTTT PTRPTAITSA SGICGLNLST FAANGSSSGG SNGGLSMTAL LPQQQQQQQQ
QHQMSQQQQQ QQQQQQQQGN SSSGQQQQPN GILACSTPKA NTPTTTQQQM YAAVMAAAAS
ASASTSGSAN SSLNNSNSTL NTSGGLNNSA SGGDDFRCNP CNKNLSSLTR LKRHIQNVHM
RPTKEPVCNI CKRVYSSLNS LRNHKSIYHR NLKQPKQEPG VGATQAAANS FYHQQHQQQQ
LNHHSSS
