ID   TERM_BP35C              Reviewed;         245 AA.
AC   Q6X3W5;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   23-FEB-2022, entry version 47.
DE   RecName: Full=DNA terminal protein;
GN   ORFNames=4;
OS   Bacillus phage Bam35c (Bacillus thuringiensis bacteriophage Bam35c).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Kalamavirales; Tectiviridae; Betatectivirus.
OX   NCBI_TaxID=236750;
OH   NCBI_TaxID=1428; Bacillus thuringiensis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=12954208; DOI=10.1016/s0042-6822(03)00295-2;
RA   Ravantti J.J., Gaidelyte A., Bamford D.H., Bamford J.K.;
RT   "Comparative analysis of bacterial viruses Bam35, infecting a gram-positive
RT   host, and PRD1, infecting gram-negative hosts, demonstrates a viral
RT   lineage.";
RL   Virology 313:401-414(2003).
RN   [2]
RP   SUBCELLULAR LOCATION, AND NUCLEAR LOCALIZATION SIGNAL.
RX   PubMed=23091024; DOI=10.1073/pnas.1216635109;
RA   Redrejo-Rodriguez M., Munoz-Espin D., Holguera I., Mencia M., Salas M.;
RT   "Functional eukaryotic nuclear localization signals are widespread in
RT   terminal proteins of bacteriophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:18482-18487(2012).
RN   [3]
RP   FUNCTION, MUTAGENESIS OF TYR-194; TYR-202; TYR-215; TYR-229; TYR-234;
RP   TYR-244 AND TYR-245, AND COVALENT DNA LINKAGE AT TYR-194.
RX   PubMed=27466389; DOI=10.1093/nar/gkw673;
RA   Berjon-Otero M., Villar L., Salas M., Redrejo-Rodriguez M.;
RT   "Disclosing early steps of protein-primed genome replication of the Gram-
RT   positive tectivirus Bam35.";
RL   Nucleic Acids Res. 44:9733-9744(2016).
RN   [4]
RP   INTERACTION WITH PROTEIN P1.
RX   PubMed=28747494; DOI=10.1128/jvi.00870-17;
RA   Berjon-Otero M., Lechuga A., Mehla J., Uetz P., Salas M.,
RA   Redrejo-Rodriguez M.;
RT   "Bam35 tectivirus intraviral interaction map unveils new function and
RT   localization of phage ORFan proteins.";
RL   J. Virol. 0:0-0(2017).
CC   -!- FUNCTION: Acts as a primer for viral genomic replication. DNA terminal
CC       protein is covalently linked to the 5'-ends of both strands of the
CC       genome through a phosphodiester bond between the beta-hydroxyl group of
CC       a tyrosine residue and the 5'-phosphate of the terminal
CC       deoxythymidylate. This protein is essential for DNA replication and is
CC       involved in the priming of DNA elongation.
CC       {ECO:0000269|PubMed:27466389}.
CC   -!- SUBUNIT: Interacts with the DNA-binding protein P1.
CC       {ECO:0000269|PubMed:28747494}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
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DR   EMBL; AY257527; AAP83474.1; -; Genomic_DNA.
DR   RefSeq; NP_943750.1; NC_005258.1.
DR   SMR; Q6X3W5; -.
DR   GeneID; 2658242; -.
DR   KEGG; vg:2658242; -.
DR   Proteomes; UP000002550; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Covalent protein-DNA linkage; DNA replication; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Viral DNA replication; Virion.
FT   CHAIN           1..245
FT                   /note="DNA terminal protein"
FT                   /id="PRO_0000445360"
FT   MOTIF           4..55
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000269|PubMed:23091024"
FT   SITE            172
FT                   /note="Interaction with the viral DNA polymerase"
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MOD_RES         194
FT                   /note="O-(5'-phospho-DNA)-tyrosine"
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         194
FT                   /note="Y->F: Loss of DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         202
FT                   /note="Y->F: No effect on DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         215
FT                   /note="Y->F: No effect on DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         229
FT                   /note="Y->F: No effect on DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         234
FT                   /note="Y->F: No effect on DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         244
FT                   /note="Y->F: No effect on DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
FT   MUTAGEN         245
FT                   /note="Y->F: No effect on DNA priming capacity."
FT                   /evidence="ECO:0000269|PubMed:27466389"
SQ   SEQUENCE   245 AA;  29207 MW;  ADE9F99F1F98D76C CRC64;
     MANKRLKKKL ETKRKKSLLV SEGYSKKETK KLKGRELETV YKKKAHNRKN RERAREIANL
     AKQWGLSPSK YNSWKKLLPE IERIKKEQDR EAPFLLIYYQ DFTGETDSKF IYDFKKRNNT
     RSRSQITESI IGWLQNAHNK LFLGRVAIRI VPKRDVSKTN TLWRNHGYVK IYEGQGKELS
     KLLTAIETIM VGVYDVKERD KYLKELVAKL RSLPYEKAKK NAKEIQKIYD TKSYKKESWD
     NDDYY