ID   TERM_BPPRD              Reviewed;         259 AA.
AC   P09009; Q3T4P5;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   23-FEB-2022, entry version 78.
DE   RecName: Full=DNA terminal protein;
DE   AltName: Full=Protein P8;
GN   Name=VIII;
OS   Enterobacteria phage PRD1 (Bacteriophage PRD1).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Kalamavirales; Tectiviridae; Alphatectivirus.
OX   NCBI_TaxID=10658;
OH   NCBI_TaxID=471; Acinetobacter calcoaceticus.
OH   NCBI_TaxID=562; Escherichia coli.
OH   NCBI_TaxID=584; Proteus mirabilis.
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
OH   NCBI_TaxID=294; Pseudomonas fluorescens.
OH   NCBI_TaxID=303; Pseudomonas putida (Arthrobacter siderocapsulatus).
OH   NCBI_TaxID=90371; Salmonella typhimurium.
OH   NCBI_TaxID=666; Vibrio cholerae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3684578; DOI=10.1093/nar/15.21.8999;
RA   Hsieh J.-C., Jung G., Leavitt M.C., Ito J.;
RT   "Primary structure of the DNA terminal protein of bacteriophage PRD1.";
RL   Nucleic Acids Res. 15:8999-9009(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3322943; DOI=10.1016/0378-1119(87)90183-1;
RA   Savilahti H., Bamford D.H.;
RT   "The complete nucleotide sequence of the left very early region of
RT   Escherichia coli bacteriophage PRD1 coding for the terminal protein and the
RT   DNA polymerase.";
RL   Gene 57:121-130(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1853567; DOI=10.1016/0042-6822(91)90995-n;
RA   Bamford J.K.H., Haenninen A.-L., Pakula T.M., Ojala P.M., Kalkkinen N.,
RA   Frilander M., Bamford D.H.;
RT   "Genome organization of membrane-containing bacteriophage PRD1.";
RL   Virology 183:658-676(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15946683; DOI=10.1016/j.jmb.2005.04.059;
RA   Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L.,
RA   Bamford D.H., Bamford J.K.H.;
RT   "A snapshot of viral evolution from genome analysis of the tectiviridae
RT   family.";
RL   J. Mol. Biol. 350:427-440(2005).
RN   [5]
RP   COVALENT DNA LINKAGE AT TYR-190.
RX   PubMed=1861973; DOI=10.1093/nar/19.14.3805;
RA   Shiue S.Y., Hsieh J.C., Ito J.;
RT   "Mapping of the DNA linking tyrosine residue of the PRD1 terminal
RT   protein.";
RL   Nucleic Acids Res. 19:3805-3810(1991).
RN   [6]
RP   FUNCTION.
RX   PubMed=20823229; DOI=10.1073/pnas.1010530107;
RA   Munoz-Espin D., Holguera I., Ballesteros-Plaza D., Carballido-Lopez R.,
RA   Salas M.;
RT   "Viral terminal protein directs early organization of phage DNA replication
RT   at the bacterial nucleoid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:16548-16553(2010).
RN   [7]
RP   SUBCELLULAR LOCATION, AND NUCLEAR LOCALIZATION SIGNAL.
RX   PubMed=23091024; DOI=10.1073/pnas.1216635109;
RA   Redrejo-Rodriguez M., Munoz-Espin D., Holguera I., Mencia M., Salas M.;
RT   "Functional eukaryotic nuclear localization signals are widespread in
RT   terminal proteins of bacteriophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:18482-18487(2012).
CC   -!- FUNCTION: Acts as a primer for viral genomic replication. DNA terminal
CC       protein is covalently linked to the 5'-ends of both strands of the
CC       genome through a phosphodiester bond between the beta-hydroxyl group of
CC       a tyrosine residue and the 5'-phosphate of the terminal deoxyadenylate.
CC       This protein is essential for DNA replication and is involved in the
CC       priming of DNA elongation (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:20823229}.
CC   -!- SUBUNIT: Heterodimer with viral polymerase. Binds to ssDNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host nucleus
CC       {ECO:0000269|PubMed:23091024}.
CC   -!- SIMILARITY: Belongs to the tectivirus DNA terminal protein family.
CC       {ECO:0000305}.
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DR   EMBL; X06321; CAA29636.1; -; Genomic_DNA.
DR   EMBL; M22161; AAA32449.1; -; Genomic_DNA.
DR   EMBL; AY848689; AAX45926.1; -; Genomic_DNA.
DR   PIR; S01613; TPBPPR.
DR   RefSeq; NP_040681.1; NC_001421.2.
DR   Proteomes; UP000002143; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0019073; P:viral DNA genome packaging; IDA:CACAO.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Covalent protein-DNA linkage; DNA replication; DNA-binding; Early protein;
KW   Host nucleus; Nucleotide-binding; Phosphoprotein; Reference proteome;
KW   Viral DNA replication; Virion.
FT   CHAIN           1..259
FT                   /note="DNA terminal protein"
FT                   /id="PRO_0000165347"
FT   MOTIF           243..259
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000269|PubMed:23091024"
FT   MOD_RES         190
FT                   /note="O-(5'-phospho-DNA)-tyrosine"
FT                   /evidence="ECO:0000269|PubMed:1861973"
SQ   SEQUENCE   259 AA;  29514 MW;  C58491546CE3B710 CRC64;
     MAKKKPVEKN GLVYKEFQKQ VSNLKKAGLI PKTLDVRKVK PTKHYKGLVS KYKDVATGGA
     KLAAIPNPAV IETLEARGES IIKKGGKAYL KARQQINQRG QIVNPFTVRV TKRGEVVRRY
     RKTTPEGKPV YITQRELPIK FENMEQWLTE LKAAGFQLQP GEQIYFTFNG NYSRRTYTSF
     DEAFNKFMTY DIIIDAVAGK LKVEDEADLV KSVGFQRISG PEAKAYNRNR IVLPEMQFSQ
     AAKKKYKRRQ KRGYGSKGV