TERS_BPMU
ID TERS_BPMU Reviewed; 191 AA.
AC Q9T1W7;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 23-FEB-2022, entry version 59.
DE RecName: Full=Probable terminase, small subunit gp27;
DE AltName: Full=Gene product 27;
DE Short=gp27;
DE AltName: Full=Gene product D;
DE Short=gpD;
DE AltName: Full=Pacase, small subunit;
DE AltName: Full=Packaging protein D;
GN OrderedLocusNames=Mup27;
OS Escherichia phage Mu (Bacteriophage Mu).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Muvirus.
OX NCBI_TaxID=10677;
OH NCBI_TaxID=543; Enterobacteriaceae.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11922669; DOI=10.1006/jmbi.2002.5437;
RA Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.;
RT "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like
RT prophages in Haemophilus, Neisseria and Deinococcus.";
RL J. Mol. Biol. 317:337-359(2002).
RN [2]
RP INDUCTION.
RX PubMed=8293968; DOI=10.1093/genetics/135.3.619;
RA Chiang L.W., Howe M.M.;
RT "Mutational analysis of a C-dependent late promoter of bacteriophage Mu.";
RL Genetics 135:619-629(1993).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=14745593; DOI=10.1007/s00705-003-0216-4;
RA Siboo I.R., Sieder F., Kumar K., Howe M.M., DuBow M.S.;
RT "Characterization of Plys-proximal morphogenetic genes of transposable
RT bacteriophage Mu.";
RL Arch. Virol. 149:241-259(2004).
CC -!- FUNCTION: The terminase small subunit binds to the packaging initiation
CC site (pac) and regulates the ATPase activity of the terminase large
CC subunit. The terminase lies at a unique vertex of the procapsid and is
CC composed of two subunits, a small terminase subunit involved in viral
CC DNA recognition (packaging sequence), and a large terminase subunit
CC possessing endonucleolytic and ATPase activities. Both terminase
CC subunits heterooligomerize and are docked on the portal protein to form
CC the packaging machine. The terminase large subunit exhibits
CC endonuclease activity and cleaves the viral genome concatemer once the
CC capsid is full (headful packaging). Once the capsid is packaged with
CC the DNA, the terminase complex is substituted by neck proteins.
CC {ECO:0000305|PubMed:14745593}.
CC -!- SUBUNIT: Heterodimer with the terminase large subunit gp28; the active
CC complex is probably heterooligomeric. {ECO:0000305|PubMed:14745593}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC Expression of late genes is activated by the viral late transcription
CC activator C. {ECO:0000269|PubMed:8293968}.
CC -!- SIMILARITY: Belongs to the mulikevirus small terminase family.
CC {ECO:0000305}.
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DR EMBL; AF083977; AAF01105.1; -; Genomic_DNA.
DR RefSeq; NP_050631.1; NC_000929.1.
DR GeneID; 2636271; -.
DR KEGG; vg:2636271; -.
DR Proteomes; UP000002611; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032359; P:provirus excision; IEA:UniProtKB-KW.
DR InterPro; IPR021874; Phage_Mu_Gp27.
DR Pfam; PF11985; DUF3486; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Host cytoplasm; Late protein; Reference proteome;
KW Viral genome excision; Viral genome packaging;
KW Viral release from host cell.
FT CHAIN 1..191
FT /note="Probable terminase, small subunit gp27"
FT /id="PRO_0000077820"
SQ SEQUENCE 191 AA; 21625 MW; 08286454756642E9 CRC64;
MDRKTRGRAS KVDLLPENVR KTLHEMLRDK AIPQARILEE INALIEDAGL PDEMKLSRSG
LNRYATNVEQ VGHNLRQMRE MTSALTAELG DKPMGETTKL ILEMARSQLF KAMMRQIENP
ESDVDIDLLK NAMLAAQRLE STAMSSHRRE KEIRQAFAEE AANAVSEELR GQDGISEELE
QRIRDVLLGK A
