ID   BRC2B_ARATH             Reviewed;        1155 AA.
AC   Q7Y1C4; Q84W87; Q9M013;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Protein BREAST CANCER SUSCEPTIBILITY 2 homolog B {ECO:0000303|PubMed:16415210};
DE            Short=AtBRCA2B {ECO:0000303|PubMed:16415210};
GN   Name=BRCA2B {ECO:0000303|PubMed:16415210};
GN   Synonyms=BRCA2(V) {ECO:0000303|PubMed:15014444};
GN   OrderedLocusNames=At5g01630 {ECO:0000312|Araport:AT5G01630};
GN   ORFNames=F7A7.150 {ECO:0000312|EMBL:CAB82279.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAD51 AND DMC1, AND
RP   TISSUE SPECIFICITY.
RX   PubMed=15014444; DOI=10.1038/sj.emboj.7600146;
RA   Siaud N., Dray E., Gy I., Gerard E., Takvorian N., Doutriaux M.P.;
RT   "Brca2 is involved in meiosis in Arabidopsis thaliana as suggested by its
RT   interaction with Dmc1.";
RL   EMBO J. 23:1392-1401(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 632-1155.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   INTERACTION WITH RAD51; DMC1; DSS1(V) AND DSS1(I).
RX   PubMed=16415210; DOI=10.1104/pp.105.075838;
RA   Dray E., Siaud N., Dubois E., Doutriaux M.P.;
RT   "Interaction between Arabidopsis Brca2 and its partners Rad51, Dmc1, and
RT   Dss1.";
RL   Plant Physiol. 140:1059-1069(2006).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=22039535; DOI=10.1371/journal.pone.0026696;
RA   Dumont M., Massot S., Doutriaux M.P., Gratias A.;
RT   "Characterization of Brca2-deficient plants excludes the role of NHEJ and
RT   SSA in the meiotic chromosomal defect phenotype.";
RL   PLoS ONE 6:E26696-E26696(2011).
RN   [7]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=22077663; DOI=10.1111/j.1469-8137.2011.03947.x;
RA   Seeliger K., Dukowic-Schulze S., Wurz-Wildersinn R., Pacher M., Puchta H.;
RT   "BRCA2 is a mediator of RAD51- and DMC1-facilitated homologous
RT   recombination in Arabidopsis thaliana.";
RL   New Phytol. 193:364-375(2012).
CC   -!- FUNCTION: Involved in double-strand break repair and/or homologous
CC       recombination by mediating RAD51- and DMC1-facilitated DNA repair.
CC       Plays an essential role in both somatic and meiotic homologous
CC       recombination. Is crucial for the formation of RAD51 and DMC1 foci
CC       during male meiotic homologous recombination in prophase I.
CC       {ECO:0000269|PubMed:15014444, ECO:0000269|PubMed:22039535,
CC       ECO:0000269|PubMed:22077663}.
CC   -!- SUBUNIT: Interacts with RAD51 and DMC1 (PubMed:15014444,
CC       PubMed:16415210). Interacts with DSS1(I) and DSS1(V) (PubMed:16415210).
CC       Can interact with both RAD51 and DSS1(I) or both DMC1 and DSS1(I) in a
CC       tripartite complex (PubMed:16415210). {ECO:0000269|PubMed:15014444,
CC       ECO:0000269|PubMed:16415210}.
CC   -!- INTERACTION:
CC       Q7Y1C4; Q39009: DMC1; NbExp=6; IntAct=EBI-307707, EBI-307715;
CC       Q7Y1C4; Q9XIR8: DSS1(I); NbExp=2; IntAct=EBI-307707, EBI-931045;
CC       Q7Y1C4; Q9FL96: DSS1(V); NbExp=2; IntAct=EBI-307707, EBI-931034;
CC       Q7Y1C4; P94102: RAD51; NbExp=5; IntAct=EBI-307707, EBI-307687;
CC   -!- TISSUE SPECIFICITY: Expressed in flower buds.
CC       {ECO:0000269|PubMed:15014444}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions, but the double mutants brca2a and brca2b are sterile due to
CC       aberrant chromosome aggregates, chromosomal fragmentation and
CC       missegregation during meiosis. {ECO:0000269|PubMed:22039535,
CC       ECO:0000269|PubMed:22077663}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB82279.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AJ488305; CAD32572.1; -; mRNA.
DR   EMBL; AL161946; CAB82279.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED90369.1; -; Genomic_DNA.
DR   EMBL; BT004117; AAO42140.1; -; mRNA.
DR   PIR; T48184; T48184.
DR   RefSeq; NP_195783.3; NM_120241.4.
DR   AlphaFoldDB; Q7Y1C4; -.
DR   SMR; Q7Y1C4; -.
DR   BioGRID; 16986; 4.
DR   IntAct; Q7Y1C4; 5.
DR   STRING; 3702.AT5G01630.1; -.
DR   iPTMnet; Q7Y1C4; -.
DR   PaxDb; Q7Y1C4; -.
DR   PRIDE; Q7Y1C4; -.
DR   ProteomicsDB; 222820; -.
DR   EnsemblPlants; AT5G01630.1; AT5G01630.1; AT5G01630.
DR   GeneID; 831710; -.
DR   Gramene; AT5G01630.1; AT5G01630.1; AT5G01630.
DR   KEGG; ath:AT5G01630; -.
DR   Araport; AT5G01630; -.
DR   TAIR; locus:2149800; AT5G01630.
DR   eggNOG; KOG4751; Eukaryota.
DR   HOGENOM; CLU_004336_0_0_1; -.
DR   InParanoid; Q7Y1C4; -.
DR   OMA; CYPTRAA; -.
DR   OrthoDB; 91604at2759; -.
DR   PhylomeDB; Q7Y1C4; -.
DR   PRO; PR:Q7Y1C4; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q7Y1C4; baseline and differential.
DR   Genevisible; Q7Y1C4; AT.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IGI:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IMP:TAIR.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   CDD; cd04493; BRCA2DBD_OB1; 1.
DR   Gene3D; 2.40.50.140; -; 4.
DR   InterPro; IPR015525; BRCA2.
DR   InterPro; IPR015252; BRCA2_hlx.
DR   InterPro; IPR036315; BRCA2_hlx_sf.
DR   InterPro; IPR015187; BRCA2_OB_1.
DR   InterPro; IPR002093; BRCA2_repeat.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   PANTHER; PTHR11289; PTHR11289; 2.
DR   Pfam; PF09169; BRCA-2_helical; 1.
DR   Pfam; PF09103; BRCA-2_OB1; 1.
DR   Pfam; PF00634; BRCA2; 3.
DR   PIRSF; PIRSF002397; BRCA2; 1.
DR   SUPFAM; SSF50249; SSF50249; 3.
DR   SUPFAM; SSF81872; SSF81872; 1.
DR   PROSITE; PS50138; BRCA2_REPEAT; 2.
PE   1: Evidence at protein level;
KW   Cell cycle; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Meiosis; Reference proteome; Repeat.
FT   CHAIN           1..1155
FT                   /note="Protein BREAST CANCER SUSCEPTIBILITY 2 homolog B"
FT                   /id="PRO_0000430160"
FT   REPEAT          63..97
FT                   /note="BRCA2 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00032"
FT   REPEAT          116..150
FT                   /note="BRCA2 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00032"
FT   REPEAT          163..197
FT                   /note="BRCA2 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00032"
FT   REPEAT          257..291
FT                   /note="BRCA2 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00032"
SQ   SEQUENCE   1155 AA;  127255 MW;  3D310C37E044C2AE CRC64;
     MSTWHLFSDS SGDGFRWEVA GRILQSVSDS TPTKALESTA PLPSMADLLL QGCSKLIERE
     ESMPGEIPMF RTGLGKSVVL KESSIAKAKS ILAENVAYSD LQNTNCSIPQ TRQVDTAETM
     PMFRTALGKT VPLKESSIAK PLSILGSDMI IDSDNVLPRE SGFGVPNSLF QTASNKKVNV
     SSAGLARAKA LLGLEEDDLN GFNHVNQSSS SLQQHGWSGL KTHEEFDATV VKHHSGTPGQ
     YENYVSGKRS EILNPSLKVP PTKFQTAGGK SLSVSAEALK RARNLLGDPE LGSFFDDVAG
     GDQFFTPQKD ERLSDIAINN GSVNTGYIAH EEKTSNKHTS NSFVSPLHSS SKQFRSVNLE
     NLASGGNLIK KFDTAVDETN CALNISKPAT HGLSNNRPLA SDMAVNNSKG NGFIPRARQL
     GRPADQPLVD ITNRRDTAYA NNKQDSTQKK RLGKTVSVSP FKRPRISSFK TPLKKNAQQA
     SSGLSVVSCD TLTSKKVLST RYPEKSPRVY IKEFFGMHPT ATTRMDYVPD HVRRIKSSNA
     DKYVFCDESS SNKVGAETFL QMLAESGASL QHASRKWVTN HYRWIVWKLA CYDIYYPAKC
     RGNFLTITNV LEELKYRYER EVNHGHCSAI KRILSGDAPA SSMMVLCISA INPRTDNGSQ
     EAHCSDNCSN VKVELTDGWY SMNAALDVVL TKQLNAGKLF VGQKLRILGA GLSGWATPTS
     PLEAVISSTI CLLLNINGTY RAHWADRLGF CKEIGVPLAF NCIKCNGGPV PKTLAGITRI
     YPILYKERLG EKKSIVRSER IESRIIQLHN QRRSALVEGI MCEYQRGING VHSQNDTDSE
     EGAKVFKLLE TAAEPELLMA EMSLEQLTSF TTYKAKFEAA KQMQMEKSVA KALEDAGLGE
     RNVTPFMRIR LVGLTSLSNE GEHNPKEGIV TIWDPTERQR TELTEGKIYI MKGLVPMNSD
     SETLYLHARG SSSRWQPLSP KDSENFQPFF NPRKPISLSN LGEIPLSSEF DIAAYVVYVG
     DAYTDVLQKK QWVFVTDGST QHSGEISNSL LAISFSTPFM DDSSVSHISH NLVGSVVGFC
     NLIKRAKDAT NEMWVAETTE NSVYFINAEA AYSSHLKTRS AHIQTWAKLY SSKSVIHELR
     QRVLFIIGAC KSPSC