ID   TERS_BPT4               Reviewed;         164 AA.
AC   P17311;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   02-DEC-2020, entry version 80.
DE   RecName: Full=Terminase, small subunit;
DE   AltName: Full=Gene product 16;
DE            Short=gp16;
DE   AltName: Full=Packaging protein Gp16;
GN   Name=16;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=D;
RX   PubMed=2374730; DOI=10.1093/nar/18.13.4005;
RA   Powell D., Franklin J., Arisaka F., Mosig G.;
RT   "Bacteriophage T4 DNA packaging genes 16 and 17.";
RL   Nucleic Acids Res. 18:4005-4005(1990).
RN   [2]
RP   SUBUNIT.
RX   PubMed=9013596; DOI=10.1074/jbc.272.6.3495;
RA   Lin H., Simon M.N., Black L.W.;
RT   "Purification and characterization of the small subunit of phage T4
RT   terminase, gp16, required for DNA packaging.";
RL   J. Biol. Chem. 272:3495-3501(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=12235385; DOI=10.1093/nar/gkf524;
RA   Mitchell M.S., Matsuzaki S., Imai S., Rao V.B.;
RT   "Sequence analysis of bacteriophage T4 DNA packaging/terminase genes 16 and
RT   17 reveals a common ATPase center in the large subunit of viral
RT   terminases.";
RL   Nucleic Acids Res. 30:4009-4021(2002).
RN   [5]
RP   INTERACTION WITH GP17.
RX   PubMed=12466275; DOI=10.1074/jbc.m208574200;
RA   Baumann R.G., Black L.W.;
RT   "Isolation and characterization of T4 bacteriophage gp17 terminase, a large
RT   subunit multimer with enhanced ATPase activity.";
RL   J. Biol. Chem. 278:4618-4627(2003).
RN   [6]
RP   FUNCTION.
RX   PubMed=19561086; DOI=10.1074/jbc.m109.025007;
RA   Al-Zahrani A.S., Kondabagil K., Gao S., Kelly N., Ghosh-Kumar M., Rao V.B.;
RT   "The small terminase, gp16, of bacteriophage T4 is a regulator of the DNA
RT   packaging motor.";
RL   J. Biol. Chem. 284:24490-24500(2009).
CC   -!- FUNCTION: The terminase small subunit binds to the packaging initiation
CC       site and regulates the ATPase activity of the terminase large subunit.
CC       The terminase lies at a unique vertex of the procapsid and is composed
CC       of two subunits, a small terminase subunit involved in viral DNA
CC       recognition (packaging sequence), and a large terminase subunit
CC       possessing endonucleolytic and ATPase activities. Both terminase
CC       subunits heterooligomerize and are docked on the portal protein to form
CC       the packaging machine. The terminase large subunit exhibits
CC       endonuclease activity and cleaves the viral genome concatemer once the
CC       capsid is full (headful packaging). Once the capsid is packaged with
CC       the DNA, the terminase complex is substituted by neck proteins.
CC       {ECO:0000305|PubMed:19561086}.
CC   -!- SUBUNIT: Homooctamer (Probable)(PubMed:9013596). Interacts with the
CC       terminase large subunit gp17; the active complex is probably
CC       heterooligomeric (PubMed:12466275). {ECO:0000269|PubMed:12466275,
CC       ECO:0000269|PubMed:9013596}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X52394; CAA36640.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42421.1; -; Genomic_DNA.
DR   PIR; JU0286; GSBPT4.
DR   RefSeq; NP_049775.1; NC_000866.4.
DR   SMR; P17311; -.
DR   DIP; DIP-60321N; -.
DR   IntAct; P17311; 1.
DR   GeneID; 1258811; -.
DR   KEGG; vg:1258811; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0043493; C:viral terminase complex; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR020342; Phage_T4_Gp16_DNA-pack.
DR   Pfam; PF11053; DNA_Packaging; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Viral genome packaging;
KW   Viral release from host cell.
FT   CHAIN           1..164
FT                   /note="Terminase, small subunit"
FT                   /id="PRO_0000165006"
FT   DNA_BIND        1..35
FT                   /evidence="ECO:0000269|PubMed:19561086"
FT   REGION          1..35
FT                   /note="Interaction with gp17"
FT                   /evidence="ECO:0000269|PubMed:19561086"
FT   REGION          36..114
FT                   /note="Oligomerization"
FT                   /evidence="ECO:0000269|PubMed:19561086"
FT   REGION          115..164
FT                   /note="Interaction with gp17"
FT                   /evidence="ECO:0000269|PubMed:19561086"
SQ   SEQUENCE   164 AA;  18388 MW;  2D26A3B563796FA5 CRC64;
     MEGLDINKLL DISDLPGIDG EEIKVYEPLQ LVEVKSNPQN RTPDLEDDYG VVRRNMHFQQ
     QMLMDAAKIF LETAKNADSP RHMEVFATLM GQMTTTNREI LKLHKDMKDI TSEQVGTKGA
     VPTGQMNIQN ATVFMGSPTE LMDEIGDAYE AQEAREKVIN GTTD