TERT_ORYSJ
ID TERT_ORYSJ Reviewed; 1259 AA.
AC Q8LKW0; Q2QTI2; Q9AU13;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Telomerase reverse transcriptase;
DE EC=2.7.7.49;
DE AltName: Full=OsTERT;
GN Name=TERT; OrderedLocusNames=Os12g0293100, LOC_Os12g19549;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Leaf;
RX PubMed=15527966; DOI=10.1016/j.gene.2004.07.011;
RA Oguchi K., Tamura K., Takahashi H.;
RT "Characterization of Oryza sativa telomerase reverse transcriptase and
RT possible role of its phosphorylation in the control of telomerase
RT activity.";
RL Gene 342:57-66(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=12100484; DOI=10.1046/j.1365-313x.2001.01337.x;
RA Heller-Uszynska K., Schnippenkoetter W., Kilian A.;
RT "Cloning and characterization of rice (Oryza sativa L) telomerase reverse
RT transcriptase, which reveals complex splicing patterns.";
RL Plant J. 31:75-86(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Telomerase is a ribonucleoprotein enzyme essential for the
CC replication of chromosome termini in most eukaryotes. It elongates
CC telomeres. It is a reverse transcriptase that adds simple sequence
CC repeats to chromosome ends by copying a template sequence within the
CC RNA component of the enzyme (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00405};
CC -!- SUBUNIT: Component of the telomerase ribonucleoprotein complex.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in shoot apices and immature embryos.
CC {ECO:0000269|PubMed:12100484, ECO:0000269|PubMed:15527966}.
CC -!- SIMILARITY: Belongs to the reverse transcriptase family. Telomerase
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM21641.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF288216; AAK35007.1; -; mRNA.
DR EMBL; AF494453; AAM21641.1; ALT_FRAME; Genomic_DNA.
DR EMBL; DP000011; ABA97494.2; -; Genomic_DNA.
DR EMBL; AP008218; BAF29629.1; -; Genomic_DNA.
DR EMBL; AP014968; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015620285.1; XM_015764799.1.
DR AlphaFoldDB; Q8LKW0; -.
DR STRING; 4530.OS12T0293100-01; -.
DR PaxDb; Q8LKW0; -.
DR PRIDE; Q8LKW0; -.
DR GeneID; 4352024; -.
DR KEGG; osa:4352024; -.
DR eggNOG; KOG1005; Eukaryota.
DR HOGENOM; CLU_710601_0_0_1; -.
DR InParanoid; Q8LKW0; -.
DR OrthoDB; 1297956at2759; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR Genevisible; Q8LKW0; OS.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0000333; C:telomerase catalytic core complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0070034; F:telomerase RNA binding; IBA:GO_Central.
DR GO; GO:0003721; F:telomerase RNA reverse transcriptase activity; IBA:GO_Central.
DR GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0007004; P:telomere maintenance via telomerase; IBA:GO_Central.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR021891; Telomerase_RBD.
DR InterPro; IPR003545; Telomerase_RT.
DR PANTHER; PTHR12066; PTHR12066; 1.
DR Pfam; PF00078; RVT_1; 1.
DR Pfam; PF12009; Telomerase_RBD; 1.
DR PRINTS; PR01365; TELOMERASERT.
DR SMART; SM00975; Telomerase_RBD; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00527; RIBOSOMAL_S14; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 2: Evidence at transcript level;
KW Chromosome; DNA-binding; Magnesium; Metal-binding; Nucleotidyltransferase;
KW Nucleus; Reference proteome; RNA-directed DNA polymerase; Telomere;
KW Transferase.
FT CHAIN 1..1259
FT /note="Telomerase reverse transcriptase"
FT /id="PRO_0000054930"
FT DOMAIN 742..1067
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT BINDING 837
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT BINDING 999
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT BINDING 1000
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT CONFLICT 34
FT /note="A -> T (in Ref. 1; AAK35007)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="V -> I (in Ref. 1; AAK35007)"
FT /evidence="ECO:0000305"
FT CONFLICT 610
FT /note="S -> F (in Ref. 1; AAK35007)"
FT /evidence="ECO:0000305"
FT CONFLICT 677
FT /note="K -> E (in Ref. 1; AAK35007)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1259 AA; 143607 MW; 11E6A4898AACE8D1 CRC64;
MPRRRRRRRA APGGQVPPEL RLAYGARALT LGRAVFSLLP SPRHCESPCP ACRGRVASGC
LACRRWEHLL RDGDPVAYRR LITRAVCAIA ADDLSAPPPP RYTPGNSGHS QARLVREMMK
SIVADQSHGT KNVLCNGLHE GGQSICISDL VSSSSWSILL HRIGDLLMCY LLRCTSIFLP
VKKNDYFQVS GVPLNVVLRN PIFASTVARK HQPQTTKAKC HTCYLWKSAN MAENLSICHD
SSNSGVNSSF SSTCKIVTQQ SCETCGSIRR AESKDPSEGC NCPKFPSDGR SGECCNCYTH
NTRKRKRLYS WQRRSKKKQV CSVDESSAEW SKLNGSNFNM SNGPSENLAG KMNDQAQSVE
LTVDNTSLAR SNDDSSSEIK VINATILSSE KSPCSVFDIR GSQGLSCHYS LSEVQYQSTC
PQVGPSSYLH LNSCSICFNC IISNASKHLS LDSLISRNGI FYNRRTTYSV FHCKHILSKR
KRPDALSLVK HIFGINSCCA SLLKYNCHES TIRKSNCLCC WLPKSIKNLI RNSKRCQYKK
LFLKHCSVKC KVAPDVTKND GKAHYPPGGK AAYYDRSFSR LEAYSTHQQV ASFVWAVLKR
IVPKPLLGNS FGKRSLRTNI WKFIKLRRFE TFQLSDCIGD LKVSHYSWLS NIEFSNCFCS
AIIGKQTGSS TSAEEQKQKN ILHCWISWLF SDIVIPVVRT YFYVTERESK RYDVFYYPKS
VWRDLTSNAI ASLNKKNFRI LRGEPRKAVR HLNCSSRVRF LPKAKDMRPL VDLRAKSKDA
NLNKCHLIMK KLRDEKPEMF GSSVFDYNNV HQNLSQFISS KRSQLMKKLK VYIVVADVSK
AFDCVSHDMV LKMIDDAFKC DEYTVRKCSK VICNRSKNSL YRFDSNASIG NGNSIYDLSI
QLSSGGGIFV DQGTICRILK EQFHHLLYEQ IKCNILKIGQ KYYLQQVGIA QGSKLSPNLC
SLYYGHLENS VLSKFLHDSK LNAGEAFSEP EYLLMRFIDD FIFISFSLEH AQKFLNRMRR
GFVFYNCYMN DSKYGFNFCA GNSEPSSNRL YRGDDGVSFM PWSGLLINCE TLEIQADYTR
YLDITIISTI TVKMHSSTKY IHSKLCHYMR PKCHPIFYDS NINSPGTIRV NIYQAFLLCA
MKFHCYIRSV SDANVSKLEL LQVIKRTFRY MHSLIVRRMQ DVELHYNVRP VLKLRRKETI
WLGLTAYIRV LQQKQSRYKD MLTLLTAELG RYCHLGHECD TLRYAVDDSH SSMFWKFKF
