TESB_ECO57
ID TESB_ECO57 Reviewed; 286 AA.
AC P0AGG3; P23911;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Acyl-CoA thioesterase 2;
DE EC=3.1.2.20 {ECO:0000250|UniProtKB:P0AGG2};
DE AltName: Full=Thioesterase II;
DE Short=TEII;
GN Name=tesB; OrderedLocusNames=Z0564, ECs0506;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Thioesterase that has relatively broad substrate specificity,
CC hydrolyzing primarily medium- and long-chain acyl-CoA substrates to
CC free fatty acids and CoA. Functions in the thioesterase-dependent
CC pathway of beta-oxidation of oleate and conjugated linoleate ((9Z,11E)-
CC octadecadienoate or CLA), which provides all energy and carbon
CC precursors required for the growth of E.coli. Thus, supports growth on
CC oleate or conjugated linoleate as the sole source of carbon by
CC hydrolyzing 3,5-tetradecadienoyl-CoA, the terminal metabolite of oleate
CC beta-oxidation via the alternative thioesterase-dependent pathway, and
CC 3,5-dodecadienoyl-CoA, the end product of CLA beta-oxidation,
CC respectively. Seems to be involved in 3-hydroxyalkanoate production in
CC E.coli. {ECO:0000250|UniProtKB:P0AGG2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + H2O = a fatty acid + CoA + H(+);
CC Xref=Rhea:RHEA:16781, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28868, ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=3.1.2.20;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16782;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate;
CC Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3E,5Z)-tetradecadienoyl-CoA + H2O = (3E,5Z)-tetradecadienoate
CC + CoA + H(+); Xref=Rhea:RHEA:55044, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:71586,
CC ChEBI:CHEBI:71590; Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55045;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+);
CC Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3Z,5E)-dodecadienoyl-CoA + H2O = (3Z,5E)-dodecadienoate + CoA
CC + H(+); Xref=Rhea:RHEA:65172, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:156333, ChEBI:CHEBI:156334;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65173;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+);
CC Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate;
CC Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate;
CC Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40116;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + pentanoyl-CoA = CoA + H(+) + pentanoate;
CC Xref=Rhea:RHEA:55052, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:31011, ChEBI:CHEBI:57287, ChEBI:CHEBI:57389;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55053;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+);
CC Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-methylpentanoyl-CoA + H2O = 4-methylpentanoate + CoA + H(+);
CC Xref=Rhea:RHEA:55064, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:74904, ChEBI:CHEBI:131445;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55065;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3R)-3-hydroxypentanoyl-CoA + H2O = (3R)-3-hydroxypentanoate +
CC CoA + H(+); Xref=Rhea:RHEA:55084, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:138587,
CC ChEBI:CHEBI:138588; Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55085;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-3-hydroxypentanoyl-CoA + H2O = (3S)-3-hydroxypentanoate +
CC CoA + H(+); Xref=Rhea:RHEA:55096, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:138607,
CC ChEBI:CHEBI:138608; Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55097;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate;
CC Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate;
CC Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+);
CC Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3R)-3-hydroxybutanoyl-CoA + H2O = (R)-3-hydroxybutanoate +
CC CoA + H(+); Xref=Rhea:RHEA:65204, ChEBI:CHEBI:10983,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57315; Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65205;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-3-hydroxybutanoyl-CoA + H2O = (S)-3-hydroxybutanoate +
CC CoA + H(+); Xref=Rhea:RHEA:65208, ChEBI:CHEBI:11047,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57316; Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65209;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-dodecenoyl-CoA + H2O = (2E)-dodecenoate + CoA + H(+);
CC Xref=Rhea:RHEA:65212, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57330, ChEBI:CHEBI:84274;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65213;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-oxododecanoyl-CoA + H2O = 3-oxododecanoate + CoA + H(+);
CC Xref=Rhea:RHEA:65216, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29743, ChEBI:CHEBI:57287, ChEBI:CHEBI:62615;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65217;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxydodecanoyl-CoA + H2O = 3-hydroxydodecanoate + CoA +
CC H(+); Xref=Rhea:RHEA:65232, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76616, ChEBI:CHEBI:156383;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65233;
CC Evidence={ECO:0000250|UniProtKB:P0AGG2};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P0AGG2}.
CC -!- SIMILARITY: Belongs to the C/M/P thioester hydrolase family.
CC {ECO:0000305}.
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DR EMBL; AE005174; AAG54802.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB33929.1; -; Genomic_DNA.
DR PIR; B90692; B90692.
DR PIR; F85542; F85542.
DR RefSeq; NP_308533.1; NC_002695.1.
DR RefSeq; WP_000075876.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AGG3; -.
DR SMR; P0AGG3; -.
DR STRING; 155864.EDL933_0527; -.
DR EnsemblBacteria; AAG54802; AAG54802; Z0564.
DR EnsemblBacteria; BAB33929; BAB33929; ECs_0506.
DR GeneID; 66671246; -.
DR GeneID; 914609; -.
DR KEGG; ece:Z0564; -.
DR KEGG; ecs:ECs_0506; -.
DR PATRIC; fig|386585.9.peg.611; -.
DR eggNOG; COG1946; Bacteria.
DR HOGENOM; CLU_032690_0_0_6; -.
DR OMA; SQVWFRT; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0102991; F:myristoyl-CoA hydrolase activity; IEA:RHEA.
DR GO; GO:0016290; F:palmitoyl-CoA hydrolase activity; IEA:RHEA.
DR GO; GO:0006637; P:acyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.40.160.210; -; 1.
DR InterPro; IPR042171; Acyl-CoA_hotdog.
DR InterPro; IPR003703; Acyl_CoA_thio.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR11066; PTHR11066; 1.
DR SUPFAM; SSF54637; SSF54637; 2.
DR TIGRFAMs; TIGR00189; tesB; 1.
PE 3: Inferred from homology;
KW Hydrolase; Lipid metabolism; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..286
FT /note="Acyl-CoA thioesterase 2"
FT /id="PRO_0000202145"
FT ACT_SITE 204
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P0AGG2"
FT ACT_SITE 228
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P0AGG2"
FT ACT_SITE 278
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P0AGG2"
SQ SEQUENCE 286 AA; 31966 MW; 8E7C4A4BCF73CA4B CRC64;
MSQALKNLLT LLNLEKIEEG LFRGQSEDLG LRQVFGGQVV GQALYAAKET VPEERLVHSF
HSYFLRPGDS KKPIIYDVET LRDGNSFSAR RVAAIQNGKP IFYMTASFQA PEAGFEHQKT
MPSAPAPDGL PSETQIAQSL AHLLPPVLKD KFICDRPLEV RPVEFHNPLK GHVAEPHRQV
WIRANGSVPD DLRVHQYLLG YASDLNFLPV ALQPHGIGFL EPGIQIATID HSMWFHRPFN
LNEWLLYSVE STSASSARGF VRGEFYTQDG VLVASTVQEG VMRNHN
