ID   TESE_COMTE              Reviewed;         264 AA.
AC   Q83VZ5;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=2-hydroxyhexa-2,4-dienoate hydratase;
DE            EC=4.2.1.132;
GN   Name=tesE;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN THE TESTOSTERONE
RP   DEGRADATION.
RC   STRAIN=TA441;
RX   PubMed=12676694; DOI=10.1128/aem.69.4.2139-2152.2003;
RA   Horinouchi M., Hayashi T., Koshino H., Yamamoto T., Kudo T.;
RT   "Gene encoding the hydrolase for the product of the meta-cleavage reaction
RT   in testosterone degradation by Comamonas testosteroni.";
RL   Appl. Environ. Microbiol. 69:2139-2152(2003).
RN   [2]
RP   FUNCTION AS A HYDRATASE, AND CATALYTIC ACTIVITY.
RX   PubMed=16151114; DOI=10.1128/aem.71.9.5275-5281.2005;
RA   Horinouchi M., Hayashi T., Koshino H., Kurita T., Kudo T.;
RT   "Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-
RT   hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related
RT   enzymes involved in testosterone degradation in Comamonas testosteroni
RT   TA441.";
RL   Appl. Environ. Microbiol. 71:5275-5281(2005).
CC   -!- FUNCTION: Involved in the catatabolism of testosterone. Catalyzes the
CC       hydration of 2-hydroxyhexa-2,4-dienoic acid to 4-hydroxy-2-oxohexanoic
CC       acid. {ECO:0000269|PubMed:12676694, ECO:0000269|PubMed:16151114}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H2O = 4-hydroxy-2-
CC         oxohexanoate; Xref=Rhea:RHEA:32535, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:53800, ChEBI:CHEBI:63693; EC=4.2.1.132;
CC         Evidence={ECO:0000269|PubMed:16151114};
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family.
CC       {ECO:0000305}.
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DR   EMBL; AB063482; BAC67694.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q83VZ5; -.
DR   SMR; Q83VZ5; -.
DR   KEGG; ag:BAC67694; -.
DR   BioCyc; MetaCyc:MON-16928; -.
DR   GO; GO:0034856; F:2-hydroxyhexa-2,4-dienoate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006706; P:steroid catabolic process; IDA:UniProtKB.
DR   Gene3D; 3.90.850.10; -; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   1: Evidence at protein level;
KW   Lipid degradation; Lipid metabolism; Lyase; Steroid metabolism.
FT   CHAIN           1..264
FT                   /note="2-hydroxyhexa-2,4-dienoate hydratase"
FT                   /id="PRO_0000418840"
SQ   SEQUENCE   264 AA;  28240 MW;  F30C984B26CBFC2D CRC64;
     MSDKQFIETQ GQRLYEALRS ARTLAPLTDN HPEMTVEDAY HISLHMLRLR EASGERVIGK
     KIGVTSKPVQ DMLNVHQPDF GFLTDSMEYE DGAAVSLKAA GLIQPRAEGE IAFMLKKDLQ
     GPGVTREDVL AATEWVAPCF EIVDSRINDW KIKIQDTVAD NASCGVFVIG KQHTDPASLD
     LAAAAMQMSK NGQPAGSGLG SAVQGHPAEA VAWLANTLGA FGIPFKAGEV ILSGSLAPLV
     PAAAGDRFDM VIEGMGTCSI QFTE