TET18_ARATH
ID TET18_ARATH Reviewed; 270 AA.
AC Q93XY5; Q9SK78;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Tetraspanin-18;
DE AltName: Full=TOM2A homologous protein 2;
GN Name=TOM2AH2; OrderedLocusNames=At2g20230; ORFNames=F11A3.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=17151019; DOI=10.1074/mcp.m600250-mcp200;
RA Jaquinod M., Villiers F., Kieffer-Jaquinod S., Hugouvieux V., Bruley C.,
RA Garin J., Bourguignon J.;
RT "A proteomics dissection of Arabidopsis thaliana vacuoles isolated from
RT cell culture.";
RL Mol. Cell. Proteomics 6:394-412(2007).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=18474569; DOI=10.1099/vir.0.2008/000539-0;
RA Fujisaki K., Kobayashi S., Tsujimoto Y., Naito S., Ishikawa M.;
RT "Analysis of tobamovirus multiplication in Arabidopsis thaliana mutants
RT defective in TOM2A homologues.";
RL J. Gen. Virol. 89:1519-1524(2008).
CC -!- FUNCTION: May be involved in the regulation of cell differentiation.
CC {ECO:0000269|PubMed:18474569}.
CC -!- FUNCTION: Promotes intracellular multiplication of tobamoviruses,
CC probably being a component of the replication complex.
CC {ECO:0000269|PubMed:18474569}.
CC -!- SUBUNIT: Homodimer. Constituent of tobamovirus replication complex (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}. Vacuole membrane {ECO:0000269|PubMed:17151019};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in rosette leaves.
CC {ECO:0000269|PubMed:18474569}.
CC -!- DISRUPTION PHENOTYPE: Slightly reduced efficiency of intracellular
CC multiplication of tobamoviruses (e.g. crucifer strain TMV-Cg),
CC characterized by a reduced amplification of TMV-related RNAs.
CC {ECO:0000269|PubMed:18474569}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AC006569; AAD21765.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC06983.1; -; Genomic_DNA.
DR EMBL; AY054570; AAK96761.1; -; mRNA.
DR EMBL; AY064662; AAL47370.1; -; mRNA.
DR EMBL; AY088475; AAM66011.1; -; mRNA.
DR PIR; F84586; F84586.
DR RefSeq; NP_565468.1; NM_127582.3.
DR AlphaFoldDB; Q93XY5; -.
DR STRING; 3702.AT2G20230.1; -.
DR TCDB; 8.A.40.5.2; the tetraspanin (tetraspanin) family.
DR iPTMnet; Q93XY5; -.
DR SwissPalm; Q93XY5; -.
DR PaxDb; Q93XY5; -.
DR PRIDE; Q93XY5; -.
DR ProteomicsDB; 232849; -.
DR EnsemblPlants; AT2G20230.1; AT2G20230.1; AT2G20230.
DR GeneID; 816542; -.
DR Gramene; AT2G20230.1; AT2G20230.1; AT2G20230.
DR KEGG; ath:AT2G20230; -.
DR Araport; AT2G20230; -.
DR TAIR; locus:2038997; AT2G20230.
DR eggNOG; ENOG502QVPS; Eukaryota.
DR HOGENOM; CLU_055730_0_0_1; -.
DR InParanoid; Q93XY5; -.
DR OMA; DHHWEKD; -.
DR OrthoDB; 1180379at2759; -.
DR PhylomeDB; Q93XY5; -.
DR PRO; PR:Q93XY5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q93XY5; baseline and differential.
DR Genevisible; Q93XY5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR PANTHER; PTHR19282; PTHR19282; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..270
FT /note="Tetraspanin-18"
FT /id="PRO_0000421058"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..99
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..183
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..270
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 212..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 245
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9C5W7"
SQ SEQUENCE 270 AA; 29721 MW; 48A4F66676C46727 CRC64;
MRRNCCHVSF ASTLKILNFV QAFIGVSIII YSIWMLHEYS RHLPVDPPPS ASSSSGTEIA
TSVSEPLKNP IDFVASIILG SNGGDHGFNL RSLDLPAPWF IYSFMAVGIL VCIVTFIGFI
AAEAINGCCL CFYSILKTLL ILLEAALVAY IAIDRHWEKD LPYDPTGELS SLRAFIEENI
DICKWVGIAV VAVQLLSLLL AMVLRAMVST PKPELDEEED DENPRSRTWD PLLGPQGNQA
PAGSSKIENW SSRIREKYGL NQSPPVNPKG
