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TET1_NAEGR
ID   TET1_NAEGR              Reviewed;         321 AA.
AC   D2W6T1;
DT   23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Tet-like dioxygenase 1 {ECO:0000303|PubMed:26323320};
DE            EC=1.14.11.n2 {ECO:0000269|PubMed:24390346, ECO:0000269|PubMed:26323320};
DE   AltName: Full=NgTet1 {ECO:0000303|PubMed:26323320};
GN   Name=TET1 {ECO:0000303|PubMed:26323320};
GN   ORFNames=NAEGRDRAFT_55029 {ECO:0000312|EMBL:EFC35221.1};
OS   Naegleria gruberi (Amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5762 {ECO:0000312|Proteomes:UP000006671};
RN   [1] {ECO:0000312|EMBL:EFC35221.1, ECO:0000312|Proteomes:UP000006671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 30224 / NEG-M;
RX   PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA   Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA   Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA   Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA   Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT   "The genome of Naegleria gruberi illuminates early eukaryotic
RT   versatility.";
RL   Cell 140:631-642(2010).
RN   [2] {ECO:0007744|PDB:4LT5}
RP   X-RAY CRYSTALLOGRAPHY (2.89 ANGSTROMS) IN COMPLEX WITH MANGANESE; SUBSTRATE
RP   ANALOG N-OXALYLGLYCINE AND 5-METHYLCYTOSINE DNA, FUNCTION, CATALYTIC
RP   ACTIVITY, COFACTOR, AND MUTAGENESIS OF ASN-147; ASP-234; HIS-297 AND
RP   GLN-310.
RX   PubMed=24390346; DOI=10.1038/nature12905;
RA   Hashimoto H., Pais J.E., Zhang X., Saleh L., Fu Z.Q., Dai N., Correa I.R.,
RA   Zheng Y., Cheng X.;
RT   "Structure of a Naegleria Tet-like dioxygenase in complex with 5-
RT   methylcytosine DNA.";
RL   Nature 506:391-395(2014).
RN   [3] {ECO:0007744|PDB:5CG8, ECO:0007744|PDB:5CG9}
RP   X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS) OF 57-321 IN COMPLEX WITH MANGANESE;
RP   2-OXOGLUTARATE AND 5-METHYLCYTOSINE DNA, CATALYTIC ACTIVITY, FUNCTION,
RP   COFACTOR, AND MUTAGENESIS OF ALA-212.
RX   PubMed=26323320; DOI=10.1093/nar/gkv870;
RA   Hashimoto H., Pais J.E., Dai N., Correa I.R., Zhang X., Zheng Y., Cheng X.;
RT   "Structure of Naegleria Tet-like dioxygenase (NgTet1) in complexes with a
RT   reaction intermediate 5-hydroxymethylcytosine DNA.";
RL   Nucleic Acids Res. 43:10713-10721(2015).
CC   -!- FUNCTION: Dioxygenase that catalyzes the conversion of the modified
CC       genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine
CC       (5hmC), and thereby plays a role in active DNA demethylation. Also
CC       mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and
CC       conversion of 5fC to 5-carboxylcytosine (5caC).
CC       {ECO:0000269|PubMed:24390346, ECO:0000269|PubMed:26323320}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + a 5-methyl-2'-deoxycytidine in DNA + O2 = a
CC         5-hydroxymethyl-2'-deoxycytidine in DNA + CO2 + succinate;
CC         Xref=Rhea:RHEA:52636, Rhea:RHEA-COMP:11370, Rhea:RHEA-COMP:13315,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:85454, ChEBI:CHEBI:136731;
CC         EC=1.14.11.n2; Evidence={ECO:0000269|PubMed:24390346,
CC         ECO:0000269|PubMed:26323320};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + a 5-hydroxymethyl-2'-deoxycytidine in DNA +
CC         O2 = a 5-formyl-2'-deoxycytidine in DNA + CO2 + H2O + succinate;
CC         Xref=Rhea:RHEA:53828, Rhea:RHEA-COMP:13315, Rhea:RHEA-COMP:13656,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:30031, ChEBI:CHEBI:136731,
CC         ChEBI:CHEBI:137731; Evidence={ECO:0000269|PubMed:24390346,
CC         ECO:0000269|PubMed:26323320};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + a 5-formyl-2'-deoxycytidine in DNA + O2 = a
CC         5-carboxyl-2'-deoxycytidine in DNA + CO2 + H(+) + succinate;
CC         Xref=Rhea:RHEA:53832, Rhea:RHEA-COMP:13656, Rhea:RHEA-COMP:13657,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:30031, ChEBI:CHEBI:137731,
CC         ChEBI:CHEBI:137732; Evidence={ECO:0000269|PubMed:24390346,
CC         ECO:0000269|PubMed:26323320};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00805,
CC         ECO:0000269|PubMed:24390346, ECO:0000269|PubMed:26323320};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU00805, ECO:0000269|PubMed:24390346,
CC       ECO:0000269|PubMed:26323320};
CC   -!- DOMAIN: Has good structural similarity to 2-oxoglutarate-dependent
CC       dioxygenases of the TET and the alkB families, in spite of very low
CC       overall sequence similarity. {ECO:0000305|PubMed:24390346}.
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DR   EMBL; GG739552; EFC35221.1; -; Genomic_DNA.
DR   PDB; 4LT5; X-ray; 2.89 A; A=2-321.
DR   PDB; 5CG8; X-ray; 2.70 A; A=57-321.
DR   PDB; 5CG9; X-ray; 2.69 A; A/D=57-321.
DR   PDBsum; 4LT5; -.
DR   PDBsum; 5CG8; -.
DR   PDBsum; 5CG9; -.
DR   AlphaFoldDB; D2W6T1; -.
DR   SMR; D2W6T1; -.
DR   EnsemblProtists; EFC35221; EFC35221; NAEGRDRAFT_55029.
DR   VEuPathDB; AmoebaDB:NAEGRDRAFT_55029; -.
DR   Proteomes; UP000006671; Unassembled WGS sequence.
DR   GO; GO:0010385; F:double-stranded methylated DNA binding; IDA:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; IDA:UniProtKB.
DR   GO; GO:0070579; F:methylcytosine dioxygenase activity; IDA:UniProtKB.
DR   GO; GO:0035511; P:oxidative DNA demethylation; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   3D-structure; Dioxygenase; DNA-binding; Iron; Metal-binding;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..321
FT                   /note="Tet-like dioxygenase 1"
FT                   /id="PRO_0000444157"
FT   DOMAIN          198..298
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT   BINDING         214
FT                   /ligand="2-oxoglutarate"
FT                   /ligand_id="ChEBI:CHEBI:16810"
FT                   /evidence="ECO:0000269|PubMed:24390346,
FT                   ECO:0000269|PubMed:26323320, ECO:0007744|PDB:4LT5,
FT                   ECO:0007744|PDB:5CG8, ECO:0007744|PDB:5CG9"
FT   BINDING         224
FT                   /ligand="2-oxoglutarate"
FT                   /ligand_id="ChEBI:CHEBI:16810"
FT                   /evidence="ECO:0000269|PubMed:24390346,
FT                   ECO:0000269|PubMed:26323320, ECO:0007744|PDB:4LT5,
FT                   ECO:0007744|PDB:5CG8, ECO:0007744|PDB:5CG9"
FT   BINDING         229
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805,
FT                   ECO:0000305|PubMed:24390346, ECO:0000305|PubMed:26323320"
FT   BINDING         231
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805,
FT                   ECO:0000305|PubMed:24390346, ECO:0000305|PubMed:26323320"
FT   BINDING         242
FT                   /ligand="2-oxoglutarate"
FT                   /ligand_id="ChEBI:CHEBI:16810"
FT                   /evidence="ECO:0000269|PubMed:24390346,
FT                   ECO:0000269|PubMed:26323320, ECO:0007744|PDB:4LT5,
FT                   ECO:0007744|PDB:5CG8, ECO:0007744|PDB:5CG9"
FT   BINDING         279
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805,
FT                   ECO:0000305|PubMed:24390346, ECO:0000305|PubMed:26323320"
FT   BINDING         289
FT                   /ligand="2-oxoglutarate"
FT                   /ligand_id="ChEBI:CHEBI:16810"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805,
FT                   ECO:0000269|PubMed:24390346, ECO:0000269|PubMed:26323320,
FT                   ECO:0007744|PDB:4LT5, ECO:0007744|PDB:5CG8,
FT                   ECO:0007744|PDB:5CG9"
FT   BINDING         310
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000269|PubMed:24390346,
FT                   ECO:0000269|PubMed:26323320"
FT   SITE            148
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000269|PubMed:24390346,
FT                   ECO:0000269|PubMed:26323320"
FT   MUTAGEN         147
FT                   /note="N->D: Reduced enzyme activity with DNA containing 5-
FT                   methylcytosine."
FT                   /evidence="ECO:0000269|PubMed:24390346"
FT   MUTAGEN         212
FT                   /note="A->F,I,L: Strongly reduced enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:26323320"
FT   MUTAGEN         212
FT                   /note="A->G: No effect on enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:26323320"
FT   MUTAGEN         212
FT                   /note="A->V: Decreases enzyme activity with DNA containing
FT                   5-hydroxymethylcytosine."
FT                   /evidence="ECO:0000269|PubMed:26323320"
FT   MUTAGEN         234
FT                   /note="D->A: Nearly abolishes enzyme activity with DNA
FT                   containing 5-methylcytosine."
FT                   /evidence="ECO:0000269|PubMed:24390346"
FT   MUTAGEN         234
FT                   /note="D->N: Strongly reduced enzyme activity with DNA
FT                   containing 5-methylcytosine."
FT                   /evidence="ECO:0000269|PubMed:24390346"
FT   MUTAGEN         297
FT                   /note="H->N: Strongly reduced enzyme activity with DNA
FT                   containing 5-methylcytosine."
FT                   /evidence="ECO:0000269|PubMed:24390346"
FT   MUTAGEN         297
FT                   /note="H->Q: Reduced enzyme activity with DNA containing 5-
FT                   methylcytosine."
FT                   /evidence="ECO:0000269|PubMed:24390346"
FT   MUTAGEN         310
FT                   /note="Q->A: Reduced enzyme activity with DNA containing 5-
FT                   methylcytosine."
FT                   /evidence="ECO:0000269|PubMed:24390346"
FT   HELIX           59..74
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          82..85
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          92..94
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          98..101
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          107..112
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           118..133
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          136..145
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          149..154
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           156..158
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           161..168
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           170..184
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           187..193
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           198..204
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          206..216
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           224..226
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          234..236
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          238..245
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          253..255
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   TURN            256..259
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          260..262
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          269..272
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   TURN            274..276
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          279..281
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          285..295
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   HELIX           298..302
FT                   /evidence="ECO:0007829|PDB:5CG9"
FT   STRAND          305..309
FT                   /evidence="ECO:0007829|PDB:5CG8"
FT   HELIX           310..320
FT                   /evidence="ECO:0007829|PDB:5CG9"
SQ   SEQUENCE   321 AA;  36498 MW;  3FEE0C3BBB3064FB CRC64;
     MTTFKQQTIK EKETKRKYCI KGTTANLTQT HPNGPVCVNR GEEVANTTTL LDSGGGINKK
     SLLQNLLSKC KTTFQQSFTN ANITLKDEKW LKNVRTAYFV CDHDGSVELA YLPNVLPKEL
     VEEFTEKFES IQTGRKKDTG YSGILDNSMP FNYVTADLSQ ELGQYLSEIV NPQINYYISK
     LLTCVSSRTI NYLVSLNDSY YALNNCLYPS TAFNSLKPSN DGHRIRKPHK DNLDITPSSL
     FYFGNFQNTE GYLELTDKNC KVFVQPGDVL FFKGNEYKHV VANITSGWRI GLVYFAHKGS
     KTKPYYEDTQ KNSLKIHKET K
 
 
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