TET5A_MOUSE
ID TET5A_MOUSE Reviewed; 447 AA.
AC D3Z5S8;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 2.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Terminal nucleotidyltransferase 5A {ECO:0000305};
DE EC=2.7.7.19 {ECO:0000269|PubMed:33882302};
GN Name=Tent5a {ECO:0000312|MGI:MGI:2670964};
GN Synonyms=Fam46a {ECO:0000312|MGI:MGI:2670964};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=19740458; DOI=10.1016/j.archoralbio.2009.08.005;
RA Etokebe G.E., Kuechler A.M., Haraldsen G., Landin M., Osmundsen H.,
RA Dembic Z.;
RT "Family-with-sequence-similarity-46, member A (Fam46a) gene is expressed in
RT developing tooth buds.";
RL Arch. Oral Biol. 54:1002-1007(2009).
RN [3]
RP MUTAGENESIS OF 157-GLU--ASN-447, TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=26803617; DOI=10.1007/s00335-016-9619-x;
RA Diener S., Bayer S., Sabrautzki S., Wieland T., Mentrup B., Przemeck G.K.,
RA Rathkolb B., Graf E., Hans W., Fuchs H., Horsch M., Schwarzmayr T.,
RA Wolf E., Klopocki E., Jakob F., Strom T.M., Hrabe de Angelis M.,
RA Lorenz-Depiereux B.;
RT "Exome sequencing identifies a nonsense mutation in Fam46a associated with
RT bone abnormalities in a new mouse model for skeletal dysplasia.";
RL Mamm. Genome 27:111-121(2016).
RN [4]
RP DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION, FUNCTION, CATALYTIC
RP ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=33882302; DOI=10.1016/j.celrep.2021.109015;
RA Gewartowska O., Aranaz-Novaliches G., Krawczyk P.S., Mroczek S.,
RA Kusio-Kobialka M., Tarkowski B., Spoutil F., Benada O., Kofronova O.,
RA Szwedziak P., Cysewski D., Gruchota J., Szpila M., Chlebowski A.,
RA Sedlacek R., Prochazka J., Dziembowski A.;
RT "Cytoplasmic polyadenylation by TENT5A is required for proper bone
RT formation.";
RL Cell Rep. 35:109015-109015(2021).
CC -!- FUNCTION: Cytoplasmic non-canonical poly(A) RNA polymerase that
CC catalyzes the transfer of one adenosine molecule from an ATP to an mRNA
CC poly(A) tail bearing a 3'-OH terminal group and participates in the
CC cytoplasmic polyadenylation (PubMed:33882302). Polyadenylates mRNA
CC encoding extracellular matrix constituents and other genes crucial for
CC bone mineralization and during osteoblast mineralization, mainly
CC focuses on ER-targeted mRNAs (PubMed:33882302, PubMed:26803617).
CC {ECO:0000269|PubMed:26803617, ECO:0000269|PubMed:33882302}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173115; EC=2.7.7.19;
CC Evidence={ECO:0000269|PubMed:33882302};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11333;
CC Evidence={ECO:0000269|PubMed:33882302};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:33882302}.
CC -!- TISSUE SPECIFICITY: Expressed in primary osteoblasts derived from
CC neonatal calvaria and osteocytes (PubMed:33882302). Strongly expressed
CC in calvaria and femur (PubMed:26803617). {ECO:0000269|PubMed:26803617,
CC ECO:0000269|PubMed:33882302}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in the dental sheet, oral
CC epithelium, and mesenchyme at E12.5; in the tooth germ bud,
CC ectomesenchyme and oral epithelium at 13.5; and in the dental papilla
CC and dental epithelium at E14.5. {ECO:0000269|PubMed:19740458}.
CC -!- INDUCTION: Increased during osteoblast differentiation.
CC {ECO:0000269|PubMed:33882302}.
CC -!- DISRUPTION PHENOTYPE: Homozygous mice lacking Tent5a have slightly
CC decreased survival, probably due to perinatal or embryonic lethality,
CC since the effect is already visible at postnatal day 6 (P6). Mutant
CC mice have an abnormal posture with frequent kyphosis and a wavy tail.
CC Mutant mice exhibit frequent bone fractures and newborn mice reveal rib
CC fractures. Adult mutant mice show decreased cartilage ossification of
CC the tail. {ECO:0000269|PubMed:33882302}.
CC -!- SIMILARITY: Belongs to the TENT family. {ECO:0000305}.
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DR EMBL; AC159809; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS52875.1; -.
DR RefSeq; NP_001153850.1; NM_001160378.1.
DR AlphaFoldDB; D3Z5S8; -.
DR SMR; D3Z5S8; -.
DR STRING; 10090.ENSMUSP00000034802; -.
DR jPOST; D3Z5S8; -.
DR MaxQB; D3Z5S8; -.
DR PaxDb; D3Z5S8; -.
DR PRIDE; D3Z5S8; -.
DR ProteomicsDB; 334921; -.
DR Antibodypedia; 46387; 107 antibodies from 20 providers.
DR Ensembl; ENSMUST00000034802; ENSMUSP00000034802; ENSMUSG00000032265.
DR GeneID; 212943; -.
DR KEGG; mmu:212943; -.
DR UCSC; uc012gxn.1; mouse.
DR CTD; 55603; -.
DR MGI; MGI:2670964; Tent5a.
DR VEuPathDB; HostDB:ENSMUSG00000032265; -.
DR eggNOG; KOG3852; Eukaryota.
DR GeneTree; ENSGT00940000157191; -.
DR InParanoid; D3Z5S8; -.
DR OMA; CCENPTM; -.
DR OrthoDB; 612201at2759; -.
DR PhylomeDB; D3Z5S8; -.
DR TreeFam; TF315239; -.
DR BioGRID-ORCS; 212943; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Tent5a; mouse.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; D3Z5S8; protein.
DR Bgee; ENSMUSG00000032265; Expressed in molar tooth and 199 other tissues.
DR ExpressionAtlas; D3Z5S8; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:1990817; F:RNA adenylyltransferase activity; IMP:UniProtKB.
DR GO; GO:0048255; P:mRNA stabilization; IBA:GO_Central.
DR GO; GO:0030501; P:positive regulation of bone mineralization; IMP:UniProtKB.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; IMP:UniProtKB.
DR GO; GO:0030278; P:regulation of ossification; IMP:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR InterPro; IPR012937; TET5.
DR PANTHER; PTHR12974; PTHR12974; 1.
DR Pfam; PF07984; NTP_transf_7; 1.
DR SMART; SM01153; DUF1693; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Transferase.
FT CHAIN 1..447
FT /note="Terminal nucleotidyltransferase 5A"
FT /id="PRO_0000454711"
FT MUTAGEN 157..447
FT /note="Missing: Homozygous mice show severe morphological
FT and skeletal abnormalities including short stature along
FT with limb, rib, pelvis, and skull deformities with minimal
FT trabecular bone and reduced cortical bone thickness in long
FT bones."
FT /evidence="ECO:0000269|PubMed:26803617"
SQ SEQUENCE 447 AA; 50793 MW; 63675AF08782CADC CRC64;
MHQRYFWTDQ GQVAFGGHYM AEGEGYFAMA EDELTGGPYI PLGGDFGGGG SSFGDRCSDY
CESPTAHCNV LNWEQVQRLD GILSETIPIH GRGNFPTLEL QPSLIVKVVR RRLEEKGIGV
RDVRLNGSAA SHVLHQDSGL GYKDLDLIFC ADLRGEEEFQ TVKDVVLDCL LDFLPEGVNK
EKITPLTLKE AYVQKMVKVC NDSDRWSLIS LSNNSGKNVE LKFVDSLRRQ FEFSVDSFQI
KLDSLLLFYE CSENPMTETF HPTIIGESVY GDFHEAFDHL CNKIIATRNP EEIRGGGLLK
YCNLLVRGFR PASEEIKTLQ RYMCSRFFID FSDIGEQQRK LESYLQNHFV GLEDRKYDYL
MTLHGVVNES TVCLMGHERR QTLNLITMLA IRVLADQNVI PNVANVTCYY QPAPYVADAN
FSNYYIAQVQ PVFTCQQQTY STWLPCN
