TET5D_HUMAN
ID TET5D_HUMAN Reviewed; 389 AA.
AC Q8NEK8; B2R9Q6; Q7Z3F6; Q8NHU1;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Terminal nucleotidyltransferase 5D {ECO:0000305};
DE EC=2.7.7.19 {ECO:0000269|PubMed:28931820, ECO:0000269|PubMed:32433990};
DE AltName: Full=Non-canonical poly(A) polymerase FAM46D {ECO:0000303|PubMed:28931820};
GN Name=TENT5D {ECO:0000312|HGNC:HGNC:28399};
GN Synonyms=FAM46D {ECO:0000312|HGNC:HGNC:28399};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-185.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-185.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=19540335; DOI=10.1016/j.ygeno.2009.06.001;
RA Bettoni F., Filho F.C., Grosso D.M., Galante P.A., Parmigiani R.B.,
RA Geraldo M.V., Henrique-Silva F., Oba-Shinjo S.M., Marie S.K., Soares F.A.,
RA Brentani H.P., Simpson A.J., de Souza S.J., Camargo A.A.;
RT "Identification of FAM46D as a novel cancer/testis antigen using EST data
RT and serological analysis.";
RL Genomics 94:153-160(2009).
RN [7]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=28931820; DOI=10.1038/s41467-017-00578-5;
RA Mroczek S., Chlebowska J., Kulinski T.M., Gewartowska O., Gruchota J.,
RA Cysewski D., Liudkovska V., Borsuk E., Nowis D., Dziembowski A.;
RT "The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in
RT multiple myeloma.";
RL Nat. Commun. 8:619-619(2017).
RN [8]
RP FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF LYS-136; GLU-158; CYS-312
RP AND LEU-313.
RX PubMed=32433990; DOI=10.1016/j.str.2020.04.023;
RA Chen H., Lu D., Shang G., Gao G., Zhang X.;
RT "Structural and Functional Analyses of the FAM46C/Plk4 Complex.";
RL Structure 28:910-921.e4(2020).
CC -!- FUNCTION: Catalyzes the transfer of one adenosine molecule from an ATP
CC to an mRNA poly(A) tail bearing a 3'-OH terminal group.
CC {ECO:0000269|PubMed:28931820, ECO:0000269|PubMed:32433990}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173115; EC=2.7.7.19;
CC Evidence={ECO:0000269|PubMed:28931820, ECO:0000269|PubMed:32433990};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11333;
CC Evidence={ECO:0000305|PubMed:32433990};
CC -!- INTERACTION:
CC Q8NEK8; Q03989: ARID5A; NbExp=3; IntAct=EBI-744726, EBI-948603;
CC Q8NEK8; Q5SWW7: C10orf55; NbExp=3; IntAct=EBI-744726, EBI-12809220;
CC Q8NEK8; Q6P1W5: C1orf94; NbExp=3; IntAct=EBI-744726, EBI-946029;
CC Q8NEK8; Q96IM9: DYDC2; NbExp=3; IntAct=EBI-744726, EBI-749277;
CC Q8NEK8; Q9Y5J3: HEY1; NbExp=3; IntAct=EBI-744726, EBI-7231130;
CC Q8NEK8; Q8IUC1: KRTAP11-1; NbExp=3; IntAct=EBI-744726, EBI-1052037;
CC Q8NEK8; Q3LHN2: KRTAP19-2; NbExp=3; IntAct=EBI-744726, EBI-12196745;
CC Q8NEK8; Q3LI66: KRTAP6-2; NbExp=5; IntAct=EBI-744726, EBI-11962084;
CC Q8NEK8; P35548: MSX2; NbExp=3; IntAct=EBI-744726, EBI-6447480;
CC Q8NEK8; Q9H361: PABPC3; NbExp=3; IntAct=EBI-744726, EBI-1055272;
CC Q8NEK8; Q58EX7: PLEKHG4; NbExp=3; IntAct=EBI-744726, EBI-949255;
CC Q8NEK8; P26045: PTPN3; NbExp=3; IntAct=EBI-744726, EBI-1047946;
CC Q8NEK8; Q86WH2: RASSF3; NbExp=3; IntAct=EBI-744726, EBI-2845202;
CC -!- TISSUE SPECIFICITY: restricted to testis.
CC {ECO:0000269|PubMed:19540335}.
CC -!- SIMILARITY: Belongs to the TENT family. {ECO:0000305}.
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DR EMBL; AK128147; BAG54638.1; -; mRNA.
DR EMBL; AK313875; BAG36603.1; -; mRNA.
DR EMBL; BX537938; CAD97909.1; -; mRNA.
DR EMBL; AL591431; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471104; EAW98586.1; -; Genomic_DNA.
DR EMBL; BC028710; AAH28710.1; -; mRNA.
DR EMBL; BC030784; AAH30784.1; -; mRNA.
DR EMBL; BC034979; AAH34979.1; -; mRNA.
DR CCDS; CCDS14446.1; -.
DR RefSeq; NP_001164045.1; NM_001170574.1.
DR RefSeq; NP_689843.1; NM_152630.4.
DR AlphaFoldDB; Q8NEK8; -.
DR SMR; Q8NEK8; -.
DR BioGRID; 127991; 15.
DR IntAct; Q8NEK8; 14.
DR STRING; 9606.ENSP00000443410; -.
DR iPTMnet; Q8NEK8; -.
DR PhosphoSitePlus; Q8NEK8; -.
DR BioMuta; FAM46D; -.
DR DMDM; 74751253; -.
DR MassIVE; Q8NEK8; -.
DR PaxDb; Q8NEK8; -.
DR PeptideAtlas; Q8NEK8; -.
DR PRIDE; Q8NEK8; -.
DR Antibodypedia; 56143; 69 antibodies from 15 providers.
DR DNASU; 169966; -.
DR Ensembl; ENST00000308293.5; ENSP00000308575.5; ENSG00000174016.11.
DR Ensembl; ENST00000538312.5; ENSP00000443410.1; ENSG00000174016.11.
DR GeneID; 169966; -.
DR KEGG; hsa:169966; -.
DR MANE-Select; ENST00000308293.6; ENSP00000308575.5; NM_152630.5; NP_689843.1.
DR UCSC; uc004edl.1; human.
DR CTD; 169966; -.
DR DisGeNET; 169966; -.
DR GeneCards; TENT5D; -.
DR HGNC; HGNC:28399; TENT5D.
DR HPA; ENSG00000174016; Tissue enriched (testis).
DR MIM; 300976; gene.
DR neXtProt; NX_Q8NEK8; -.
DR OpenTargets; ENSG00000174016; -.
DR PharmGKB; PA134947605; -.
DR VEuPathDB; HostDB:ENSG00000174016; -.
DR eggNOG; KOG3852; Eukaryota.
DR GeneTree; ENSGT00940000162488; -.
DR HOGENOM; CLU_008115_2_0_1; -.
DR InParanoid; Q8NEK8; -.
DR OMA; TWDQIKI; -.
DR OrthoDB; 612201at2759; -.
DR PhylomeDB; Q8NEK8; -.
DR TreeFam; TF315239; -.
DR BRENDA; 2.7.7.19; 2681.
DR PathwayCommons; Q8NEK8; -.
DR SignaLink; Q8NEK8; -.
DR BioGRID-ORCS; 169966; 19 hits in 692 CRISPR screens.
DR GenomeRNAi; 169966; -.
DR Pharos; Q8NEK8; Tbio.
DR PRO; PR:Q8NEK8; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q8NEK8; protein.
DR Bgee; ENSG00000174016; Expressed in sperm and 7 other tissues.
DR Genevisible; Q8NEK8; HS.
DR GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IMP:UniProtKB.
DR GO; GO:1990817; F:RNA adenylyltransferase activity; IDA:UniProtKB.
DR GO; GO:0048255; P:mRNA stabilization; IBA:GO_Central.
DR InterPro; IPR012937; TET5.
DR PANTHER; PTHR12974; PTHR12974; 1.
DR Pfam; PF07984; NTP_transf_7; 1.
DR SMART; SM01153; DUF1693; 1.
PE 1: Evidence at protein level;
KW Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1..389
FT /note="Terminal nucleotidyltransferase 5D"
FT /id="PRO_0000259939"
FT VARIANT 185
FT /note="D -> E (in dbSNP:rs1113265)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:17974005"
FT /id="VAR_028980"
FT MUTAGEN 136
FT /note="K->P: Decrease about 50% the poly(A) elongation
FT activity; when associated with E-312 and E-313."
FT /evidence="ECO:0000269|PubMed:32433990"
FT MUTAGEN 158
FT /note="E->Q: Loss of poly(A) elongation activity."
FT /evidence="ECO:0000269|PubMed:32433990"
FT MUTAGEN 312
FT /note="C->E: Does not affect poly (A) elongation activity;
FT when associated with E-313. Decrease about 50% the poly(A)
FT elongation activity; when associated with P-136 and E-313."
FT /evidence="ECO:0000269|PubMed:32433990"
FT MUTAGEN 313
FT /note="L->E: Does not affect poly (A) elongation activity;
FT when associated with E-312. Decrease about 50% the poly(A)
FT elongation activity; when associated with P-136 and E-312."
FT /evidence="ECO:0000269|PubMed:32433990"
FT CONFLICT 10
FT /note="T -> A (in Ref. 5; AAH28710)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="F -> L (in Ref. 5; AAH28710)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 389 AA; 44500 MW; F7E117DF03203418 CRC64;
MSEIRFTNLT WDQVITLDQV LDEVIPIHGK GNFPTMEVKP KDIIHVVKDQ LIGQGIIVKD
ARLNGSVASY ILASHNGISY KDLDVIFGVE LPGNEEFQVV KDAVLDCLLD FLPKDVKKEK
LSPDIMKDAY VQKLVKVCNG HDCWSLISLS NNTGKNLELK FVSSLRRQFE FSVDSFQIVL
DPMLDFYSDK NAKLTKESYP VVVAESMYGD FQEAMTHLQH KLICTRKPEE IRGGGLLKYC
SLLVHGFKPA CMSEIKNLER YMCSRFFIDF PHIEEQQKKI ESYLHNHFIG EGMTKYDYLM
TLHGVVNEST VCLMSYERRQ ILHLITMMAL KVLGELNILP NTQKVTCFYQ PAPYFAAEAR
YPIYVIPEPP PVSFQPYHPL HFRGSNGMS
