ID   TETM_STRLI              Reviewed;         639 AA.
AC   Q02652;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Tetracycline resistance protein TetM;
GN   Name=tetM; Synonyms=tet;
OS   Streptomyces lividans.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1916;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=66 / 1326;
RX   PubMed=1510403; DOI=10.1128/aac.36.5.1119;
RA   Dittrich W., Schrempf H.;
RT   "The unstable tetracycline resistance gene of Streptomyces lividans 1326
RT   encodes a putative protein with similarities to translational elongation
RT   factors and Tet(M) and Tet(O) proteins.";
RL   Antimicrob. Agents Chemother. 36:1119-1124(1992).
CC   -!- FUNCTION: Abolishes the inhibitory effect of tetracyclin on protein
CC       synthesis by a non-covalent modification of the ribosomes.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. TetM/TetO
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU01059}.
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DR   EMBL; M74049; AAA26830.1; -; Genomic_DNA.
DR   PIR; A48900; A48900.
DR   AlphaFoldDB; Q02652; -.
DR   SMR; Q02652; -.
DR   KEGG; ag:AAA26830; -.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   CDD; cd03711; Tet_C; 1.
DR   Gene3D; 3.30.230.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR035647; EFG_III/V.
DR   InterPro; IPR000640; EFG_V-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR035650; Tet_C.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR   Pfam; PF00679; EFG_C; 1.
DR   Pfam; PF03764; EFG_IV; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SMART; SM00889; EFG_IV; 1.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   SUPFAM; SSF54980; SSF54980; 2.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; GTP-binding; Nucleotide-binding;
KW   Protein biosynthesis.
FT   CHAIN           1..639
FT                   /note="Tetracycline resistance protein TetM"
FT                   /id="PRO_0000091501"
FT   DOMAIN          1..247
FT                   /note="tr-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT   REGION          247..267
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          611..639
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         10..17
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         74..78
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         128..131
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   639 AA;  67172 MW;  947FEBEA55BD5E1A CRC64;
     MRTLNIGILA HVDAGKTSLT ERLLFDHGAV DRLGSVDAGD TRTVDGGIER RRGITIRSAV
     AAFTVGDTRV NLIDTPGHSD FVAEVERALE VLDGAVLLLS AVEGVQARTR VLMRALRRLR
     LPTIVFVNKI DRAGARTDGL LGDVRRLLTP HVAPLTEVAD AGTPRARVTR RPPDGRTAEA
     LAEVDTEVLA ALVDGPEPTG EDVARALAAR TADGSFHPLY HGSALGGQGV AELVEGLLGL
     IPAATPGTSG GTSGGTEPRG TVFAVRPGPA GERTAYLRLY GGEVHPRRRL TFLRRESDGR
     TTEVSGRVTR LDVVGGDATL TAGNIAALTV PGGLRVGDRL GGPTDRAPQF APPTLQTLVR
     ARHPEQAAPL RSALLALADQ DPLLHARPAA SGATALLLYG EVQMEVLAAT LAEDFGIEAE
     FTPGRVRFLE RPAGTDEAAE EMPWLDRTRY FATIGLRVEP GPRGSGGAFG YETELGALPR
     AFHQAVEETV HDTLRTGLTG AAVTDYRVTL IRSGFSSPLS TAADFRGLTP LVLRRALARA
     GTVLHEPYQA FEAEVPADTL AAVTALLASL GADFTGTTGG DPAWIVTGEL PARRVREAEL
     RLPGLTHGEA VWSSRPCEDR PLKAGNSGPG TGVGGHSGE