ID   TETR3_ECOLX             Reviewed;         219 AA.
AC   P03039;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Tetracycline repressor protein class C;
GN   Name=tetR;
OS   Escherichia coli.
OG   Plasmid pSC101.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3916707; DOI=10.1093/oxfordjournals.molbev.a040334;
RA   Brow M.A.D., Pesin R., Sutcliffe J.G.;
RT   "The tetracycline repressor of pSC101.";
RL   Mol. Biol. Evol. 2:1-12(1985).
CC   -!- FUNCTION: TetR is the repressor of the tetracycline resistance element;
CC       its N-terminal region forms a helix-turn-helix structure and binds DNA.
CC       Binding of tetracycline to TetR reduces the repressor affinity for the
CC       tetracycline resistance gene (tetA) promoter operator sites.
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DR   EMBL; M36272; AAA25677.1; -; Genomic_DNA.
DR   PIR; A03575; RPECYS.
DR   RefSeq; WP_001038045.1; NZ_UIKY01000081.1.
DR   AlphaFoldDB; P03039; -.
DR   SMR; P03039; -.
DR   PRIDE; P03039; -.
DR   GeneID; 64209528; -.
DR   OMA; SALYWHF; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR023772; DNA-bd_HTH_TetR-type_CS.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR004111; Repressor_TetR_C.
DR   InterPro; IPR003012; Tet_transcr_reg_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF02909; TetR_C_1; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   PRINTS; PR00400; TETREPRESSOR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS01081; HTH_TETR_1; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   4: Predicted;
KW   Antibiotic resistance; DNA-binding; Magnesium; Metal-binding; Plasmid;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..219
FT                   /note="Tetracycline repressor protein class C"
FT                   /id="PRO_0000070614"
FT   DOMAIN          3..63
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        26..45
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   BINDING         64
FT                   /ligand="tetracycline"
FT                   /ligand_id="ChEBI:CHEBI:77932"
FT                   /evidence="ECO:0000250|UniProtKB:P0ACT4"
FT   BINDING         82
FT                   /ligand="tetracycline"
FT                   /ligand_id="ChEBI:CHEBI:77932"
FT                   /evidence="ECO:0000250|UniProtKB:P0ACT4"
FT   BINDING         100
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:P0ACT4"
SQ   SEQUENCE   219 AA;  24174 MW;  57DC010979A94879 CRC64;
     MNKLQREAVI RTALELLNDV GMEGLTTRRL AERLGVQQPA LYWHFKNKRA LLDALAEAML
     TINHTHSTPR DDDDWRSFLK GNACSFRRAL LAYRDGARIH AGTRPAAPQM EKADAQLRFL
     CDAGFSAGDA TYALMAISYF TVGAVLEQQA SEADAEERGE DQLTTSASTM PARLQSAMKI
     VYEGGPDAAF ERGLALIIGG LEKMRLTTND IEVLKNVDE