TETR_MYCA9
ID TETR_MYCA9 Reviewed; 190 AA.
AC B1MM06;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=DNA-binding transcriptional repressor TetR {ECO:0000303|PubMed:29632012};
DE AltName: Full=MabTetRx {ECO:0000303|PubMed:29632012};
GN Name=tetR {ECO:0000303|PubMed:29632012}; OrderedLocusNames=MAB_1497c;
OS Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM
OS 13569 / NCTC 13031 / TMC 1543) (Mycobacterium abscessus).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacteroides; Mycobacteroides abscessus.
OX NCBI_TaxID=561007;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC
RC 1543;
RX PubMed=19543527; DOI=10.1371/journal.pone.0005660;
RA Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M.,
RA Macheras E., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L.,
RA Gaillard J.L.;
RT "Non mycobacterial virulence genes in the genome of the emerging pathogen
RT Mycobacterium abscessus.";
RL PLoS ONE 4:E5660-E5660(2009).
RN [2]
RP FUNCTION, INDUCTION BY TETRACYCLINE, OPERON, AND DNA-BINDING.
RX PubMed=29632012; DOI=10.1128/aac.00119-18;
RA Rudra P., Hurst-Hess K., Lappierre P., Ghosh P.;
RT "High Levels of Intrinsic Tetracycline Resistance in Mycobacterium
RT abscessus Are Conferred by a Tetracycline-Modifying Monooxygenase.";
RL Antimicrob. Agents Chemother. 62:0-0(2018).
CC -!- FUNCTION: Binds to its own palindromic promoter and represses
CC transcription of its operon; addition of tetracycline or doxycycline
CC (but not tigecycline) interferes with DNA binding. Addition of TetX to
CC the DNA-TetR-antibiotic complex restores DNA binding.
CC {ECO:0000269|PubMed:29632012}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INDUCTION: Expression strongly increases after 30 minutes and then
CC decreases over 3 hours in the presence of tetracycline; not under
CC control of whiB7. Probably part of the TetR-TetX operon.
CC {ECO:0000269|PubMed:29632012}.
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DR EMBL; CU458896; CAM61583.1; -; Genomic_DNA.
DR RefSeq; WP_005084713.1; NZ_MLCG01000002.1.
DR AlphaFoldDB; B1MM06; -.
DR SMR; B1MM06; -.
DR EnsemblBacteria; CAM61583; CAM61583; MAB_1497c.
DR GeneID; 66971882; -.
DR KEGG; mab:MAB_1497c; -.
DR OMA; EMVIGPM; -.
DR Proteomes; UP000007137; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProt.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR023772; DNA-bd_HTH_TetR-type_CS.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001647; HTH_TetR.
DR InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR InterPro; IPR011075; TetR_C.
DR Pfam; PF16859; TetR_C_11; 1.
DR Pfam; PF00440; TetR_N; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF48498; SSF48498; 1.
DR PROSITE; PS01081; HTH_TETR_1; 1.
DR PROSITE; PS50977; HTH_TETR_2; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; DNA-binding; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..190
FT /note="DNA-binding transcriptional repressor TetR"
FT /id="PRO_0000448377"
FT DOMAIN 6..66
FT /note="HTH tetR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT DNA_BIND 29..48
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ SEQUENCE 190 AA; 20721 MW; 6EA58284D6594CE6 CRC64;
MVARGETRSA ALLAVALQVL IRDGYDRFSM DSVAALAHAS KTTIYRRWSN KAELIKAALD
AHDASFNDEV FDTGGLRTDL IATMGMLRRK AQALPPTLYP DLIRAMEHDE TLSDAIRRHL
ADPGLSPFDA PLSRAVGRGE AGVDVDRQLI HDVAEAMLTH RLTLGGPLDD AFIGRLVDDV
LLVLIRPGAR
