TEX35_HUMAN
ID TEX35_HUMAN Reviewed; 233 AA.
AC Q5T0J7; Q5T0J8; Q5T0J9; Q8IYU6; Q8N7Z1; Q8NA15; Q9H0Q7;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Testis-expressed protein 35;
GN Name=TEX35; Synonyms=C1orf49;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Fetal brain;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), AND VARIANT
RP ARG-171.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=17077512; DOI=10.1248/bpb.29.2187;
RA Tang A., Yu Z., Gui Y., Guo X., Long Y., Cai Z.;
RT "Identification and characteristics of a novel testis-specific gene, Tsc24,
RT in human and mice.";
RL Biol. Pharm. Bull. 29:2187-2191(2006).
CC -!- INTERACTION:
CC Q5T0J7-2; P07550: ADRB2; NbExp=3; IntAct=EBI-12833746, EBI-491169;
CC Q5T0J7-2; Q9NWQ9: C14orf119; NbExp=3; IntAct=EBI-12833746, EBI-725606;
CC Q5T0J7-2; P22607: FGFR3; NbExp=3; IntAct=EBI-12833746, EBI-348399;
CC Q5T0J7-2; Q53GS7: GLE1; NbExp=3; IntAct=EBI-12833746, EBI-1955541;
CC Q5T0J7-2; Q14957: GRIN2C; NbExp=3; IntAct=EBI-12833746, EBI-8285963;
CC Q5T0J7-2; P06396: GSN; NbExp=3; IntAct=EBI-12833746, EBI-351506;
CC Q5T0J7-2; P01112: HRAS; NbExp=3; IntAct=EBI-12833746, EBI-350145;
CC Q5T0J7-2; P61970: NUTF2; NbExp=3; IntAct=EBI-12833746, EBI-591778;
CC Q5T0J7-2; P07237: P4HB; NbExp=3; IntAct=EBI-12833746, EBI-395883;
CC Q5T0J7-2; P60201-2: PLP1; NbExp=3; IntAct=EBI-12833746, EBI-12188331;
CC Q5T0J7-2; Q01105-2: SET; NbExp=3; IntAct=EBI-12833746, EBI-7481343;
CC Q5T0J7-2; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-12833746, EBI-5235340;
CC Q5T0J7-2; Q9P1I4: ST13; NbExp=3; IntAct=EBI-12833746, EBI-25892254;
CC Q5T0J7-2; Q9BZL1: UBL5; NbExp=3; IntAct=EBI-12833746, EBI-607755;
CC Q5T0J7-2; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-12833746, EBI-741480;
CC Q5T0J7-2; Q9Y3C0: WASHC3; NbExp=3; IntAct=EBI-12833746, EBI-712969;
CC Q5T0J7-2; Q9Y649; NbExp=3; IntAct=EBI-12833746, EBI-25900580;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q5T0J7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5T0J7-2; Sequence=VSP_020738, VSP_020740;
CC Name=3;
CC IsoId=Q5T0J7-3; Sequence=VSP_020739;
CC Name=4;
CC IsoId=Q5T0J7-4; Sequence=VSP_020737, VSP_020741;
CC Name=5;
CC IsoId=Q5T0J7-5; Sequence=VSP_045739;
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:17077512}.
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DR EMBL; AL136694; CAB66629.1; -; mRNA.
DR EMBL; AK093269; BAC04115.1; -; mRNA.
DR EMBL; AK097518; BAC05085.1; -; mRNA.
DR EMBL; AL513013; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC034972; AAH34972.1; -; mRNA.
DR CCDS; CCDS1323.1; -. [Q5T0J7-1]
DR CCDS; CCDS53433.1; -. [Q5T0J7-5]
DR CCDS; CCDS53434.1; -. [Q5T0J7-2]
DR RefSeq; NP_001164193.1; NM_001170722.1. [Q5T0J7-2]
DR RefSeq; NP_001164194.1; NM_001170723.1. [Q5T0J7-5]
DR RefSeq; NP_001164195.1; NM_001170724.1.
DR RefSeq; NP_115502.2; NM_032126.4. [Q5T0J7-1]
DR AlphaFoldDB; Q5T0J7; -.
DR SMR; Q5T0J7; -.
DR BioGRID; 123861; 35.
DR IntAct; Q5T0J7; 43.
DR MINT; Q5T0J7; -.
DR STRING; 9606.ENSP00000323795; -.
DR iPTMnet; Q5T0J7; -.
DR PhosphoSitePlus; Q5T0J7; -.
DR BioMuta; TEX35; -.
DR DMDM; 74744310; -.
DR jPOST; Q5T0J7; -.
DR MassIVE; Q5T0J7; -.
DR PaxDb; Q5T0J7; -.
DR PeptideAtlas; Q5T0J7; -.
DR PRIDE; Q5T0J7; -.
DR ProteomicsDB; 64172; -. [Q5T0J7-1]
DR ProteomicsDB; 64173; -. [Q5T0J7-2]
DR ProteomicsDB; 64174; -. [Q5T0J7-3]
DR ProteomicsDB; 64176; -.
DR Antibodypedia; 50038; 96 antibodies from 15 providers.
DR DNASU; 84066; -.
DR Ensembl; ENST00000319416.7; ENSP00000323795.2; ENSG00000240021.10. [Q5T0J7-1]
DR Ensembl; ENST00000367639.1; ENSP00000356611.1; ENSG00000240021.10. [Q5T0J7-2]
DR Ensembl; ENST00000367641.7; ENSP00000356613.3; ENSG00000240021.10. [Q5T0J7-3]
DR Ensembl; ENST00000367643.7; ENSP00000356615.3; ENSG00000240021.10. [Q5T0J7-5]
DR GeneID; 84066; -.
DR KEGG; hsa:84066; -.
DR MANE-Select; ENST00000319416.7; ENSP00000323795.2; NM_032126.5; NP_115502.2.
DR UCSC; uc001glt.3; human. [Q5T0J7-1]
DR CTD; 84066; -.
DR DisGeNET; 84066; -.
DR GeneCards; TEX35; -.
DR HGNC; HGNC:25366; TEX35.
DR HPA; ENSG00000240021; Tissue enriched (testis).
DR neXtProt; NX_Q5T0J7; -.
DR OpenTargets; ENSG00000240021; -.
DR PharmGKB; PA134904440; -.
DR VEuPathDB; HostDB:ENSG00000240021; -.
DR eggNOG; ENOG502RVNR; Eukaryota.
DR GeneTree; ENSGT00390000011962; -.
DR HOGENOM; CLU_086070_0_0_1; -.
DR InParanoid; Q5T0J7; -.
DR OMA; KCILCAL; -.
DR OrthoDB; 1400673at2759; -.
DR PhylomeDB; Q5T0J7; -.
DR TreeFam; TF337831; -.
DR PathwayCommons; Q5T0J7; -.
DR SignaLink; Q5T0J7; -.
DR BioGRID-ORCS; 84066; 18 hits in 1070 CRISPR screens.
DR ChiTaRS; TEX35; human.
DR GeneWiki; C1orf49; -.
DR GenomeRNAi; 84066; -.
DR Pharos; Q5T0J7; Tdark.
DR PRO; PR:Q5T0J7; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q5T0J7; protein.
DR Bgee; ENSG00000240021; Expressed in left testis and 112 other tissues.
DR ExpressionAtlas; Q5T0J7; baseline and differential.
DR Genevisible; Q5T0J7; HS.
DR GO; GO:0015630; C:microtubule cytoskeleton; IDA:LIFEdb.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR InterPro; IPR027874; Tex35.
DR PANTHER; PTHR36860; PTHR36860; 1.
DR Pfam; PF15079; Tsc35; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Reference proteome.
FT CHAIN 1..233
FT /note="Testis-expressed protein 35"
FT /id="PRO_0000251187"
FT REGION 199..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 45..111
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..76
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_020737"
FT VAR_SEQ 13
FT /note="L -> LVKERHCWT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020738"
FT VAR_SEQ 182..233
FT /note="EKCLLCALKNNYNRGNIPSEASGLYKGGEEPVTTQPSVGHAVPAPKSQTEGR
FT -> VSETLPEPSTGARPLAPAPW (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_020739"
FT VAR_SEQ 196..233
FT /note="GNIPSEASGLYKGGEEPVTTQPSVGHAVPAPKSQTEGR -> GIYAAVGLLD
FT LW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020740"
FT VAR_SEQ 196..233
FT /note="GNIPSEASGLYKGGEEPVTTQPSVGHAVPAPKSQTEGR -> AAPLKEARHL
FT LTANSIDPSAALVLLIEFLLLTLVTGAAAPGLDACGHQRNMRDEINSERR (in
FT isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_020741"
FT VAR_SEQ 196..233
FT /note="GNIPSEASGLYKGGEEPVTTQPSVGHAVPAPKSQTEGR -> AQLQNLPAVP
FT IHHLQAP (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_045739"
FT VARIANT 55
FT /note="E -> G (in dbSNP:rs16852957)"
FT /id="VAR_027656"
FT VARIANT 146
FT /note="A -> G (in dbSNP:rs12079481)"
FT /id="VAR_027657"
FT VARIANT 171
FT /note="L -> P (in dbSNP:rs3813636)"
FT /id="VAR_059592"
FT VARIANT 171
FT /note="L -> R (in dbSNP:rs3813636)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_027658"
FT CONFLICT 14
FT /note="S -> C (in Ref. 1; CAB66629)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 233 AA; 26518 MW; 0B8B9168E1809B05 CRC64;
MSAKRAELKK THLSKNYKAV CLELKPEPTK TFDYKAVKQE GRFTKAGVTQ DLKNELREVR
EELKEKMEEI KQIKDLMDKD FDKLHEFVEI MKEMQKDMDE KMDILINTQK NYKLPLRRAP
KEQQELRLMG KTHREPQLRP KKMDGASGVN GAPCALHKKT MAPQKTKQGS LDPLHHCGTC
CEKCLLCALK NNYNRGNIPS EASGLYKGGE EPVTTQPSVG HAVPAPKSQT EGR
