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BRCC3_BOVIN
ID   BRCC3_BOVIN             Reviewed;         316 AA.
AC   A5PJP6; Q58D60;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Lys-63-specific deubiquitinase BRCC36;
DE            EC=3.4.19.- {ECO:0000250|UniProtKB:P46736};
DE   AltName: Full=BRCA1-A complex subunit BRCC36;
DE   AltName: Full=BRCA1/BRCA2-containing complex subunit 3;
DE   AltName: Full=BRCA1/BRCA2-containing complex subunit 36;
DE   AltName: Full=BRISC complex subunit BRCC36;
GN   Name=BRCC3; Synonyms=BRCC36;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=Hereford; TISSUE=Hypothalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Metalloprotease that specifically cleaves 'Lys-63'-linked
CC       polyubiquitin chains. Does not have activity toward 'Lys-48'-linked
CC       polyubiquitin chains. Component of the BRCA1-A complex, a complex that
CC       specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and
CC       H2AX at DNA lesions sites, leading to target the BRCA1-BARD1
CC       heterodimer to sites of DNA damage at double-strand breaks (DSBs). In
CC       the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin
CC       on histones H2A and H2AX, antagonizing the RNF8-dependent
CC       ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the
CC       BRISC complex, a multiprotein complex that specifically cleaves 'Lys-
CC       63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-
CC       63'-specific deubiquitination associated with the COP9 signalosome
CC       complex (CSN), via the interaction of the BRISC complex with the CSN
CC       complex. The BRISC complex is required for normal mitotic spindle
CC       assembly and microtubule attachment to kinetochores via its role in
CC       deubiquitinating NUMA1. Plays a role in interferon signaling via its
CC       role in the deubiquitination of the interferon receptor IFNAR1;
CC       deubiquitination increases IFNAR1 activity by enhancing its stability
CC       and cell surface expression. Down-regulates the response to bacterial
CC       lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (By
CC       similarity). Deubiquitinates HDAC1 and PWWP2B leading to their
CC       stabilization (By similarity). {ECO:0000250|UniProtKB:P46736,
CC       ECO:0000250|UniProtKB:P46737}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:E2AXC7,
CC         ECO:0000250|UniProtKB:P46736};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:E2AXC7};
CC   -!- SUBUNIT: Component of the ARISC complex, at least composed of
CC       UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component
CC       of the BRCA1-A complex, at least composed of BRCA1, BARD1, UIMC1/RAP80,
CC       ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex,
CC       interacts directly with ABRAXAS1 and BABAM2. Component of the BRISC
CC       complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and
CC       BABAM1/NBA1. Identified in a complex with SHMT2 and the other subunits
CC       of the BRISC complex. In the BRISC complex, interacts directly with
CC       ABRAXAS2. Identified in a complex with ABRAXAS2 and NUMA1. The BRISC
CC       complex interacts with the CSN complex. Component of the BRCA1/BRCA2
CC       containing complex (BRCC), which also contains BRCA1, BRCA2, BARD1,
CC       BABAM2 and RAD51. BRCC is a ubiquitin E3 ligase complex that enhances
CC       cellular survival following DNA damage. Interacts with BRCA1. Binds
CC       polyubiquitin (By similarity). Interacts with PWWP2B (By similarity).
CC       Interacts with HDAC1; this interaction is enhanced in the presence of
CC       PWWP2B (By similarity). {ECO:0000250|UniProtKB:P46736,
CC       ECO:0000250|UniProtKB:P46737}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P46736}. Cytoplasm
CC       {ECO:0000250|UniProtKB:P46736}. Cytoplasm, cytoskeleton, spindle pole
CC       {ECO:0000250|UniProtKB:P46736}. Note=Localizes at sites of DNA damage
CC       at double-strand breaks (DSBs). Interaction with ABRAXAS2 retains BRCC3
CC       in the cytoplasm. {ECO:0000250|UniProtKB:P46736}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A5PJP6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A5PJP6-2; Sequence=VSP_037283;
CC   -!- SIMILARITY: Belongs to the peptidase M67A family. BRCC36 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BT021737; AAX46584.1; -; mRNA.
DR   EMBL; BC142193; AAI42194.1; -; mRNA.
DR   RefSeq; NP_001069258.1; NM_001075790.1. [A5PJP6-2]
DR   RefSeq; XP_005227704.1; XM_005227647.3. [A5PJP6-1]
DR   RefSeq; XP_005227706.1; XM_005227649.3. [A5PJP6-2]
DR   AlphaFoldDB; A5PJP6; -.
DR   SMR; A5PJP6; -.
DR   BioGRID; 176131; 1.
DR   STRING; 9913.ENSBTAP00000010280; -.
DR   MEROPS; M67.004; -.
DR   PaxDb; A5PJP6; -.
DR   PRIDE; A5PJP6; -.
DR   Ensembl; ENSBTAT00000010280; ENSBTAP00000010280; ENSBTAG00000007817. [A5PJP6-1]
DR   GeneID; 519513; -.
DR   KEGG; bta:519513; -.
DR   CTD; 79184; -.
DR   VEuPathDB; HostDB:ENSBTAG00000007817; -.
DR   eggNOG; KOG1555; Eukaryota.
DR   GeneTree; ENSGT00390000000360; -.
DR   InParanoid; A5PJP6; -.
DR   OMA; CIGEIDT; -.
DR   OrthoDB; 968461at2759; -.
DR   Proteomes; UP000009136; Chromosome X.
DR   Bgee; ENSBTAG00000007817; Expressed in liver and 108 other tissues.
DR   ExpressionAtlas; A5PJP6; baseline.
DR   GO; GO:0070531; C:BRCA1-A complex; ISS:UniProtKB.
DR   GO; GO:0070552; C:BRISC complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000152; C:nuclear ubiquitin ligase complex; IEA:Ensembl.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:InterPro.
DR   GO; GO:0030234; F:enzyme regulator activity; IEA:Ensembl.
DR   GO; GO:0070122; F:isopeptidase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0140492; F:metal-dependent deubiquitinase activity; ISS:UniProtKB.
DR   GO; GO:0008237; F:metallopeptidase activity; ISS:UniProtKB.
DR   GO; GO:0031593; F:polyubiquitin modification-dependent protein binding; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071479; P:cellular response to ionizing radiation; IEA:Ensembl.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0006302; P:double-strand break repair; ISS:UniProtKB.
DR   GO; GO:0070537; P:histone H2A K63-linked deubiquitination; ISS:UniProtKB.
DR   GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; ISS:UniProtKB.
DR   GO; GO:0045739; P:positive regulation of DNA repair; ISS:UniProtKB.
DR   GO; GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
DR   GO; GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB.
DR   GO; GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
DR   GO; GO:0010165; P:response to X-ray; IEA:Ensembl.
DR   CDD; cd08068; MPN_BRCC36; 1.
DR   InterPro; IPR040749; BRCC36_C.
DR   InterPro; IPR000555; JAMM/MPN+_dom.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR033860; MPN_BRCC36.
DR   Pfam; PF18110; BRCC36_C; 1.
DR   Pfam; PF01398; JAB; 1.
DR   SMART; SM00232; JAB_MPN; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Alternative splicing; Cell cycle; Cell division;
KW   Chromatin regulator; Cytoplasm; Cytoskeleton; DNA damage; DNA repair;
KW   Hydrolase; Metal-binding; Metalloprotease; Mitosis; Nucleus;
KW   Phosphoprotein; Protease; Reference proteome; Ubl conjugation pathway;
KW   Zinc.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P46736"
FT   CHAIN           2..316
FT                   /note="Lys-63-specific deubiquitinase BRCC36"
FT                   /id="PRO_0000373944"
FT   DOMAIN          12..179
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   MOTIF           122..135
FT                   /note="JAMM motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   BINDING         122
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   BINDING         124
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   BINDING         135
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:P46736"
FT   MOD_RES         258
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P46736"
FT   VAR_SEQ         184..208
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16305752"
FT                   /id="VSP_037283"
SQ   SEQUENCE   316 AA;  36152 MW;  FF06B75667E7FF7C CRC64;
     MAVQVVQAVQ AVHLESDAFL VCLNHALSTE KEEVMGLCIG ELNDDLRNDP KFTYTGTEMR
     TVAEKVDTVR IVHIHSVIIL RRSDKRKDRV EISPEQLSAA STEAERLAEL TGRPMRVVGW
     YHSHPHITVW PSHVDVRTQA MYQMMDQGFV GLIFSCFIED KNTKTGRVLY TCFQSIQAQK
     SSESPRGPRD FWSSSQHISI EGQKEEERYE RIEIPIHIVP HVTIGKVCLE SAVELPKILC
     QEEQDAYRRI HSLTHLDSVT KIHNGSVFTK NLCSQMSAVS GPLLQWLEDR LEQNQQHVQE
     LQQEKEELLQ ELSSLE
 
 
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