TF2AY_MOUSE
ID TF2AY_MOUSE Reviewed; 468 AA.
AC Q8R4I4; Q99PM2; Q9DAF4;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=TFIIA-alpha and beta-like factor;
DE AltName: Full=General transcription factor II A, 1-like factor;
GN Name=Gtf2a1l; Synonyms=Alf, Gtf2a1lf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=CD-1; TISSUE=Testis;
RX PubMed=11159353; DOI=10.1095/biolreprod64.2.507;
RA Han S., Zhou L., Upadhyaya A.B., Lee S.H., Parker K.L., DeJong J.;
RT "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose
RT expression is up-regulated with other general transcription factors during
RT spermatogenesis in the mouse.";
RL Biol. Reprod. 64:507-517(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=11889132; DOI=10.1074/jbc.m200954200;
RA Xie W., Han S., Khan M., DeJong J.;
RT "Regulation of ALF gene expression in somatic and male germ line tissues
RT involves partial and site-specific patterns of methylation.";
RL J. Biol. Chem. 277:17765-17774(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May function as a testis specific transcription factor. Binds
CC DNA in conjunction with GTF2A2 and TBP (the TATA-binding protein) and
CC together with GTF2A2, allows mRNA transcription.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Testis specific. Expressed in pachytene
CC spermatocytes and haploid spermatids. {ECO:0000269|PubMed:11159353,
CC ECO:0000269|PubMed:11889132}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 14 days postnatal day.
CC {ECO:0000269|PubMed:11159353}.
CC -!- SIMILARITY: Belongs to the TFIIA subunit 1 family. {ECO:0000305}.
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DR EMBL; AF250835; AAG50432.1; -; mRNA.
DR EMBL; AF452125; AAM12730.1; -; Genomic_DNA.
DR EMBL; AK005881; BAB24296.1; -; mRNA.
DR EMBL; BC050756; AAH50756.1; -; mRNA.
DR CCDS; CCDS29024.1; -.
DR RefSeq; NP_076119.2; NM_023630.2.
DR AlphaFoldDB; Q8R4I4; -.
DR BioGRID; 214957; 8.
DR IntAct; Q8R4I4; 8.
DR MINT; Q8R4I4; -.
DR STRING; 10090.ENSMUSP00000024970; -.
DR PhosphoSitePlus; Q8R4I4; -.
DR PaxDb; Q8R4I4; -.
DR PRIDE; Q8R4I4; -.
DR ProteomicsDB; 259010; -.
DR Antibodypedia; 35034; 269 antibodies from 18 providers.
DR DNASU; 71828; -.
DR Ensembl; ENSMUST00000024970; ENSMUSP00000024970; ENSMUSG00000024154.
DR GeneID; 71828; -.
DR KEGG; mmu:71828; -.
DR UCSC; uc008dvt.2; mouse.
DR CTD; 11036; -.
DR MGI; MGI:1919078; Gtf2a1l.
DR VEuPathDB; HostDB:ENSMUSG00000024154; -.
DR eggNOG; KOG2652; Eukaryota.
DR GeneTree; ENSGT00940000163055; -.
DR HOGENOM; CLU_030027_3_1_1; -.
DR InParanoid; Q8R4I4; -.
DR OMA; CFNPVPK; -.
DR OrthoDB; 1443035at2759; -.
DR PhylomeDB; Q8R4I4; -.
DR TreeFam; TF350445; -.
DR BioGRID-ORCS; 71828; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q8R4I4; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8R4I4; protein.
DR Bgee; ENSMUSG00000024154; Expressed in seminiferous tubule of testis and 17 other tissues.
DR ExpressionAtlas; Q8R4I4; baseline and differential.
DR Genevisible; Q8R4I4; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:MGI.
DR GO; GO:0005672; C:transcription factor TFIIA complex; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0050890; P:cognition; ISO:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro.
DR Gene3D; 2.30.18.10; -; 1.
DR InterPro; IPR004855; TFIIA_asu/bsu.
DR InterPro; IPR009088; TFIIA_b-brl.
DR PANTHER; PTHR12694; PTHR12694; 1.
DR Pfam; PF03153; TFIIA; 1.
DR SMART; SM01371; TFIIA; 1.
DR SUPFAM; SSF50784; SSF50784; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..468
FT /note="TFIIA-alpha and beta-like factor"
FT /id="PRO_0000072496"
FT REGION 215..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 379..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 379..395
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 229
FT /note="S -> G (in Ref. 1; AAG50432)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 468 AA; 51525 MW; EEE36B01A6B85B63 CRC64;
MAFINLVPKL YQSVIEDVIE GVRDLFAEEG IEEQVLKDLK KLWETKVLQS KATEDFFRNS
TQVPLLTLQL PHALPPALQP EASLLIPAGR TLPSFTPEDL NTANCGANFA FAGYPIHVPA
GMAFQTASGH LYKVNVPVMV TQTSGRTEIL QHPFQQVLQQ LGQPLVIQTT VPTLHPCSLQ
AATEKSLRME AVLQPPPILH PPPVDRTHVE NAASDRRLLP GNELRPQESS PYLSLPGVGF
PPQAALTESS LEPVLGVSAS LTQNLHSDPF SQGPPGPLHH HLLESQLQSL KDSIYGCDST
KQLRKAEEPS SLRVSEKNCT SERDLNIRVT DDDINEIIQI DGTGDNSSTE EMGSIRDADE
NEFPGIIDAG DLNVLEEVDS VSNEDSTANS SDNEDHQINA PEEDPLNSGD DVSEQDVPDL
FDTENVIVCQ YDKIHRSKNR WKFYLKDGVM CFGGRDYVFA KAIGEAEW
