TF2B_CAEEL
ID TF2B_CAEEL Reviewed; 306 AA.
AC O16991;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Transcription initiation factor IIB;
DE AltName: Full=General transcription factor TFIIB;
GN Name=ttb-1; ORFNames=W03F9.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11566890; DOI=10.1093/emboj/20.18.5269;
RA Walker A.K., Rothman J.H., Shi Y., Blackwell T.K.;
RT "Distinct requirements for C.elegans TAF(II)s in early embryonic
RT transcription.";
RL EMBO J. 20:5269-5279(2001).
CC -!- FUNCTION: General transcription factor that plays a role in
CC transcription initiation by RNA polymerase II (Pol II) (By similarity).
CC Involved in the pre-initiation complex (PIC) formation and Pol II
CC recruitment at promoter DNA (By similarity). Essential for early
CC embryonic development (PubMed:11566890). {ECO:0000250|UniProtKB:Q00403,
CC ECO:0000269|PubMed:11566890}.
CC -!- SUBUNIT: Associates with TFIID-TFIIA (DA complex) to form TFIID-IIA-IIB
CC (DAB-complex) which is then recognized by RNA polymerase II.
CC {ECO:0000250|UniProtKB:Q00403}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q00403}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes arrested
CC development at 90-100 cells and inhibits differentiation
CC (PubMed:11566890). The two E cell daughters (E2 cells), which form the
CC endoderm, divide abnormally early, immediately after the MS2 cells
CC (PubMed:11566890). Phosphorylation of the RNA Pol II large subunit C-
CC terminal domain (CTD) is reduced severely in embryos (PubMed:11566890).
CC {ECO:0000269|PubMed:11566890}.
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
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DR EMBL; FO081767; CCD74279.1; -; Genomic_DNA.
DR RefSeq; NP_503146.1; NM_070745.4.
DR AlphaFoldDB; O16991; -.
DR SMR; O16991; -.
DR BioGRID; 43619; 17.
DR STRING; 6239.W03F9.5.2; -.
DR EPD; O16991; -.
DR PaxDb; O16991; -.
DR PeptideAtlas; O16991; -.
DR EnsemblMetazoa; W03F9.5.1; W03F9.5.1; WBGene00006648.
DR EnsemblMetazoa; W03F9.5.2; W03F9.5.2; WBGene00006648.
DR EnsemblMetazoa; W03F9.5.3; W03F9.5.3; WBGene00006648.
DR GeneID; 178544; -.
DR KEGG; cel:CELE_W03F9.5; -.
DR UCSC; W03F9.5.1; c. elegans.
DR CTD; 178544; -.
DR WormBase; W03F9.5; CE27396; WBGene00006648; ttb-1.
DR eggNOG; KOG1597; Eukaryota.
DR GeneTree; ENSGT00390000006671; -.
DR HOGENOM; CLU_043736_1_1_1; -.
DR InParanoid; O16991; -.
DR OMA; DHDQRMK; -.
DR OrthoDB; 729732at2759; -.
DR PhylomeDB; O16991; -.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-CEL-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-CEL-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-CEL-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-CEL-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR PRO; PR:O16991; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00006648; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR CDD; cd00043; CYCLIN; 2.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR023486; TFIIB_CS.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS00782; TFIIB; 2.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..306
FT /note="Transcription initiation factor IIB"
FT /id="PRO_0000119298"
FT REPEAT 114..190
FT /note="1"
FT REPEAT 208..284
FT /note="2"
FT ZN_FING 3..34
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT REGION 47..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 7
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 10
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 26
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 29
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ SEQUENCE 306 AA; 33102 MW; ACA6B94A8A5A8D59 CRC64;
MSAPVQCPIH PDVHLIEDHR AGDLVCPACG LVVGDRLVDV GTEWRSFSNE RSGNDPSRVG
APENPLLSGG DLSTTIAVGF GGSDSDNSLA NAQRKSMNNT DRQMTAAMSL IREMSERIHL
PRNIQDSASR IFKDVLESKA LRGKNNEAQA AACLYIACRK DGVPRTFKEI CAVSRVSKKE
IGRCFKIIVR SLETNLEQIT SADFMSRFCG NLSLPNSIQA AATRIAKCAV DMDLVAGRTP
ISIAAAAIYM ASQASAEKRS AKEIGDVAGA AEITVRQTYK LLYPKALELF PKDFRFVTPI
DALPNS
