TF2B_DICDI
ID TF2B_DICDI Reviewed; 325 AA.
AC Q54FD6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Transcription initiation factor IIB {ECO:0000250|UniProtKB:Q00403};
DE AltName: Full=General transcription factor TFIIB {ECO:0000250|UniProtKB:Q00403};
GN Name=gtf2b; Synonyms=tfiib; ORFNames=DDB_G0290929;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: General transcription factor that plays a role in
CC transcription initiation by RNA polymerase II (Pol II). Involved in the
CC pre-initiation complex (PIC) formation and Pol II recruitment at
CC promoter DNA. {ECO:0000250|UniProtKB:Q00403}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q00403}.
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
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DR EMBL; AAFI02000172; EAL61980.1; -; Genomic_DNA.
DR RefSeq; XP_635486.1; XM_630394.1.
DR AlphaFoldDB; Q54FD6; -.
DR SMR; Q54FD6; -.
DR STRING; 44689.DDB0216283; -.
DR PaxDb; Q54FD6; -.
DR EnsemblProtists; EAL61980; EAL61980; DDB_G0290929.
DR GeneID; 8627902; -.
DR KEGG; ddi:DDB_G0290929; -.
DR dictyBase; DDB_G0290929; gtf2b.
DR eggNOG; KOG1597; Eukaryota.
DR HOGENOM; CLU_043736_1_1_1; -.
DR InParanoid; Q54FD6; -.
DR OMA; DHDQRMK; -.
DR PhylomeDB; Q54FD6; -.
DR Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DDI-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR PRO; PR:Q54FD6; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005634; C:nucleus; ISS:dictyBase.
DR GO; GO:0032993; C:protein-DNA complex; ISS:UniProtKB.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0000993; F:RNA polymerase II complex binding; ISS:UniProtKB.
DR GO; GO:0001139; F:RNA polymerase II complex recruiting activity; ISS:dictyBase.
DR GO; GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:1990114; P:RNA polymerase II core complex assembly; ISS:UniProtKB.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; ISS:UniProtKB.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:UniProtKB.
DR GO; GO:0001174; P:transcriptional start site selection at RNA polymerase II promoter; ISS:UniProtKB.
DR CDD; cd00043; CYCLIN; 2.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR023486; TFIIB_CS.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS00782; TFIIB; 1.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..325
FT /note="Transcription initiation factor IIB"
FT /id="PRO_0000328160"
FT REPEAT 131..207
FT /note="1"
FT REPEAT 227..303
FT /note="2"
FT ZN_FING 19..52
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 23
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 26
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 44
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 47
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ SEQUENCE 325 AA; 35949 MW; 30DDE9AD6F4CAA53 CRC64;
MLSAAPKPTP QNKKQSFYNK LWCMVCRIQD PDIIEDYAKG DLICRGCGVV VGDRIVDEHS
EWRTFSNSES TGADPNRVGG PINPLLRDSA LSTTVGKGSK DSGTLTRLQN KSALGTGDRN
LLAAFKEIGR MADHMNLPQT VQDRANELFR FMDDKKSTKG RSVDGMVAAA LYIACRQEHL
SRTFKEIAAL TNVPKKEISR CYKIMKETFA SVLNLQTISS EDFTSRFCST LKLPNDVKKG
AEHVSKMAMD MGIVAGKSPI SVTAASIYMV SQLSPEKRTQ KQIADVSGVS EVTIRNAYKD
LYAKRDQLIP SDSPYFSQVQ YLPTN
