ID   TF2B_ENCCU              Reviewed;         312 AA.
AC   Q8SRP3;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Transcription initiation factor IIB;
DE   AltName: Full=General transcription factor TFIIB;
GN   OrderedLocusNames=ECU06_1100i;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
CC   -!- FUNCTION: General factor that plays a major role in the activation of
CC       eukaryotic genes transcribed by RNA polymerase II. {ECO:0000250}.
CC   -!- SUBUNIT: Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB
CC       (DAB-complex) which is then recognized by polymerase II.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
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DR   EMBL; AL590446; CAD25470.1; -; Genomic_DNA.
DR   RefSeq; NP_585866.1; NM_001041488.1.
DR   AlphaFoldDB; Q8SRP3; -.
DR   SMR; Q8SRP3; -.
DR   STRING; 284813.Q8SRP3; -.
DR   PRIDE; Q8SRP3; -.
DR   GeneID; 859291; -.
DR   KEGG; ecu:ECU06_1100i; -.
DR   VEuPathDB; MicrosporidiaDB:ECU06_1100i; -.
DR   HOGENOM; CLU_043736_1_1_1; -.
DR   InParanoid; Q8SRP3; -.
DR   OMA; RTQKDFA; -.
DR   OrthoDB; 729732at2759; -.
DR   Proteomes; UP000000819; Chromosome VI.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR   GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR   CDD; cd00043; CYCLIN; 2.
DR   InterPro; IPR013763; Cyclin-like.
DR   InterPro; IPR036915; Cyclin-like_sf.
DR   InterPro; IPR000812; TFIIB.
DR   InterPro; IPR023486; TFIIB_CS.
DR   InterPro; IPR013150; TFIIB_cyclin.
DR   InterPro; IPR013137; Znf_TFIIB.
DR   PANTHER; PTHR11618; PTHR11618; 1.
DR   Pfam; PF08271; TF_Zn_Ribbon; 1.
DR   Pfam; PF00382; TFIIB; 2.
DR   PRINTS; PR00685; TIFACTORIIB.
DR   SMART; SM00385; CYCLIN; 2.
DR   SUPFAM; SSF47954; SSF47954; 2.
DR   PROSITE; PS00782; TFIIB; 1.
DR   PROSITE; PS51134; ZF_TFIIB; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..312
FT                   /note="Transcription initiation factor IIB"
FT                   /id="PRO_0000119305"
FT   REPEAT          120..196
FT                   /note="1"
FT   REPEAT          213..290
FT                   /note="2"
FT   ZN_FING         10..42
FT                   /note="TFIIB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         14
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         17
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         34
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         37
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ   SEQUENCE   312 AA;  35165 MW;  E1577DCD36EC987C CRC64;
     MKTLPKFKPF VQTCSDCGET QNIVEDYKNG YHVCGRCGCI VGNRIIDEGS EWRSFGDSNK
     VDPCRIGSAS NPYLESEQLD TMISTGGGMN SYVLSKIQMK NSMRGPERAL KHGMNLITAF
     CERSNLSRTI IDRAHYIFKN IEERKLLKGK NVEGIVAACI YIACRQEECP RTFKEISVMT
     AVQKREIGRC FKLISPHLER MATMSTENII ARFCSDLNLN IKIQKIATEI AKAAHELGCL
     AGKSPDSIAA AVIYMVTNLF PEEKKIQKDI QFVTNVTEVT IKNTYKELLT FKYDIIPENM
     VNKESIDKLP GY