TF2B_KLULA
ID TF2B_KLULA Reviewed; 357 AA.
AC Q05959;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Transcription initiation factor IIB;
DE AltName: Full=General transcription factor TFIIB;
GN Name=SUA7; OrderedLocusNames=KLLA0D13640g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8346020; DOI=10.1093/nar/21.15.3413;
RA Na J.G., Hampsey M.;
RT "The Kluyveromyces gene encoding the general transcription factor IIB:
RT structural analysis and expression in Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 21:3413-3417(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: General factor that plays a major role in the activation of
CC eukaryotic genes transcribed by RNA polymerase II.
CC -!- SUBUNIT: Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB
CC (DAB-complex) which is then recognized by polymerase II.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
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DR EMBL; L11606; AAA35258.1; -; Genomic_DNA.
DR EMBL; CR382124; CAH00767.1; -; Genomic_DNA.
DR PIR; S34953; S34953.
DR RefSeq; XP_453671.1; XM_453671.1.
DR AlphaFoldDB; Q05959; -.
DR SMR; Q05959; -.
DR STRING; 28985.XP_453671.1; -.
DR EnsemblFungi; CAH00767; CAH00767; KLLA0_D13640g.
DR GeneID; 2893512; -.
DR KEGG; kla:KLLA0_D13640g; -.
DR eggNOG; KOG1597; Eukaryota.
DR HOGENOM; CLU_043736_1_0_1; -.
DR InParanoid; Q05959; -.
DR OMA; DHDQRMK; -.
DR Proteomes; UP000000598; Chromosome D.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0097550; C:transcription preinitiation complex; IEA:EnsemblFungi.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001139; F:RNA polymerase II complex recruiting activity; IEA:EnsemblFungi.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IEA:EnsemblFungi.
DR GO; GO:0017025; F:TBP-class protein binding; IEA:EnsemblFungi.
DR GO; GO:2000679; P:positive regulation of transcription regulatory region DNA binding; IEA:EnsemblFungi.
DR GO; GO:0001113; P:transcription open complex formation at RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0001174; P:transcriptional start site selection at RNA polymerase II promoter; IEA:EnsemblFungi.
DR CDD; cd00043; CYCLIN; 2.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR023486; TFIIB_CS.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS00782; TFIIB; 2.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..357
FT /note="Transcription initiation factor IIB"
FT /id="PRO_0000119306"
FT REPEAT 140..216
FT /note="1"
FT REPEAT 248..324
FT /note="2"
FT ZN_FING 23..57
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT REGION 69..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 28
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 31
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 49
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 52
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ SEQUENCE 357 AA; 39148 MW; BD12819972080FA0 CRC64;
MSLPAAASAN VAVSSRRRGP NLNIVISCPE CKVYPPKVVE RFSEGDIVCA LCGLVLSDRI
VDTRSEWRTF SNDDQNGDDP SRVGEASNPL LDGNHLTTRI GQAQGSDARV TRDLNRAQSK
SIVDKKDNEL QAAYAKITMM CDAAELPKIV KDCAKEAYKL CFEERVLKGK SQESIMASVI
LVGCRRAEVG RSFKEILSLT NVRKKEIGKT FTIIKNILRE KNDRGFANID TSNITTGQTS
AETFIPRFCS HLGLSVQVAN AAEYIAKHSK DVNVLAGRSP ITIAAAAIYM ATLLFKLNIS
PTRISQTLQV TEGTVKGGYK ALYEHKEKVV DPQLIESGVV KWDNLPRVSN DKSEKAN
