TF2B_METJA
ID TF2B_METJA Reviewed; 673 AA.
AC Q58192;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Transcription initiation factor IIB;
DE Short=TFIIB;
DE Contains:
DE RecName: Full=Endonuclease Mja Tfb;
DE EC=3.1.-.-;
DE AltName: Full=Mja TFIIB intein;
DE AltName: Full=Mja Tfb intein;
GN Name=tfb; OrderedLocusNames=MJ0782;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Stabilizes TBP binding to an archaeal box-A promoter. Also
CC responsible for recruiting RNA polymerase II to the pre-initiation
CC complex (DNA-TBP-TFIIB).
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
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DR EMBL; L77117; AAB98771.1; -; Genomic_DNA.
DR PIR; F64397; F64397.
DR PDB; 5O9J; X-ray; 2.00 A; A/B=101-225, A/B=356-435.
DR PDBsum; 5O9J; -.
DR AlphaFoldDB; Q58192; -.
DR SMR; Q58192; -.
DR STRING; 243232.MJ_0782; -.
DR MEROPS; N10.007; -.
DR PRIDE; Q58192; -.
DR EnsemblBacteria; AAB98771; AAB98771; MJ_0782.
DR KEGG; mja:MJ_0782; -.
DR eggNOG; arCOG01981; Archaea.
DR HOGENOM; CLU_026356_0_0_2; -.
DR InParanoid; Q58192; -.
DR OMA; HNTIHDK; -.
DR OrthoDB; 11535at2157; -.
DR PhylomeDB; Q58192; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR CDD; cd00043; CYCLIN; 2.
DR HAMAP; MF_00383; TF2B_arch; 1.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR023484; TFIIB_arc.
DR InterPro; IPR023486; TFIIB_CS.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00379; INTEIN.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF47954; SSF47954; 2.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS00782; TFIIB; 2.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Autocatalytic cleavage; Endonuclease; Hydrolase;
KW Intron homing; Metal-binding; Nuclease; Protein splicing;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..99
FT /note="Transcription initiation factor IIB, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000033642"
FT CHAIN 100..434
FT /note="Endonuclease Mja Tfb"
FT /evidence="ECO:0000255"
FT /id="PRO_0000033643"
FT CHAIN 435..673
FT /note="Transcription initiation factor IIB, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000033644"
FT DOMAIN 238..357
FT /note="DOD-type homing endonuclease"
FT REPEAT 490..573
FT /note="1"
FT REPEAT 584..665
FT /note="2"
FT ZN_FING 38..69
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 42
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 45
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 64
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT STRAND 106..111
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 114..119
FT /evidence="ECO:0007829|PDB:5O9J"
FT HELIX 120..129
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 134..137
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 140..144
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 146..153
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 159..163
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 166..171
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 175..180
FT /evidence="ECO:0007829|PDB:5O9J"
FT HELIX 181..183
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 184..188
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 192..198
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 201..206
FT /evidence="ECO:0007829|PDB:5O9J"
FT HELIX 207..209
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 215..225
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 394..405
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 408..416
FT /evidence="ECO:0007829|PDB:5O9J"
FT TURN 418..420
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 422..429
FT /evidence="ECO:0007829|PDB:5O9J"
FT STRAND 431..434
FT /evidence="ECO:0007829|PDB:5O9J"
SQ SEQUENCE 673 AA; 76969 MW; 20EA4713EAB031A9 CRC64;
MVWLMEALKT KENETTKEKK LTTKVEKSEK KEENVREEEI VCPICGSKEV VKDYERAEIV
CAKCGCVIKE KLFDIGPEWR AFDHEQKIKR CRVGAPMTYS VDYNEPIIIK ENGEIKVVKI
GELIDKIIEN SENIRREGIL EIAKCKGIEV IAFNSNYKFK FMPVSEVSRH PVSEMFEIVV
EGNKKVRVTR SHSVFTIRDN EVVPIRVDEL KVGDILVLAK ELPNIEEDIE IDKKFSKILG
YIIAEGYYDD KKIVLSYDYN EKEFINETID YFKSLNSDIT IYSKDLNIQI EVKNKKIINL
LKKLRVKNKR IPSIIFKSPY EIKKSFIDGI FNGKDAKVFV SKELAEDVIF LLLQIKENAT
INKKSINDIE VYEVRRITNI YTNRKLEKLI NSDFIFLKIK EINKVEPTSG YAYDLTVPNA
ENFVAGFGGF VLHNTIHDKG LSTVIDWRNK DSYGKDLSAN KRAQLYRLRK WQRRIRVSDA
AERNLAFALS ELDRITSKLG LPRHVRENAA IIYRGAVEKG LIRGRSIEGV VAAAIYAACR
RCRVPRTLDE IAEASRVDRK EIGRTYRFLA RELNIKLTPT NPIDYVPRFA SELGLPGEVE
SKAIQILQQA AEKGLTSGRG PTGVAAAAIY IASVLLGCRR TQREVAEVAG VTEVTIRNRY
KELTEHLDID VTL
