ID   TF2B_METMA              Reviewed;         337 AA.
AC   P0CW15; Q977U3;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Transcription initiation factor IIB {ECO:0000255|HAMAP-Rule:MF_00383};
DE            Short=TFIIB {ECO:0000255|HAMAP-Rule:MF_00383};
GN   Name=tfb {ECO:0000255|HAMAP-Rule:MF_00383}; OrderedLocusNames=MM_1772;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- FUNCTION: Stabilizes TBP binding to an archaeal box-A promoter. Also
CC       responsible for recruiting RNA polymerase II to the pre-initiation
CC       complex (DNA-TBP-TFIIB). {ECO:0000255|HAMAP-Rule:MF_00383}.
CC   -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00383}.
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DR   EMBL; AE008384; AAM31468.1; -; Genomic_DNA.
DR   RefSeq; WP_011033712.1; NC_003901.1.
DR   AlphaFoldDB; P0CW15; -.
DR   SMR; P0CW15; -.
DR   STRING; 192952.MM_1772; -.
DR   EnsemblBacteria; AAM31468; AAM31468; MM_1772.
DR   GeneID; 44088592; -.
DR   GeneID; 66136747; -.
DR   KEGG; mma:MM_1772; -.
DR   PATRIC; fig|192952.21.peg.2048; -.
DR   eggNOG; arCOG01981; Archaea.
DR   HOGENOM; CLU_043736_0_1_2; -.
DR   OMA; DHDQRMK; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR   CDD; cd00043; CYCLIN; 2.
DR   HAMAP; MF_00383; TF2B_arch; 1.
DR   InterPro; IPR013763; Cyclin-like.
DR   InterPro; IPR036915; Cyclin-like_sf.
DR   InterPro; IPR000812; TFIIB.
DR   InterPro; IPR023484; TFIIB_arc.
DR   InterPro; IPR023486; TFIIB_CS.
DR   InterPro; IPR013150; TFIIB_cyclin.
DR   InterPro; IPR013137; Znf_TFIIB.
DR   PANTHER; PTHR11618; PTHR11618; 1.
DR   Pfam; PF08271; TF_Zn_Ribbon; 1.
DR   Pfam; PF00382; TFIIB; 2.
DR   PRINTS; PR00685; TIFACTORIIB.
DR   SMART; SM00385; CYCLIN; 2.
DR   SUPFAM; SSF47954; SSF47954; 2.
DR   PROSITE; PS00782; TFIIB; 2.
DR   PROSITE; PS51134; ZF_TFIIB; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..337
FT                   /note="Transcription initiation factor IIB"
FT                   /id="PRO_0000408040"
FT   REPEAT          154..237
FT                   /note="1"
FT   REPEAT          248..329
FT                   /note="2"
FT   ZN_FING         37..68
FT                   /note="TFIIB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         41
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         44
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         60
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         63
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ   SEQUENCE   337 AA;  38290 MW;  39C58CA8D9ACD879 CRC64;
     MVEVERVRYS DTLEREKIRA MIKARKEKQK EQTVENEKAV CPECGSRNLV HDYERAELVC
     GDCGLVIDAD FVDEGPEWRA FDHDQRMKRS RVGAPMTYTI HDKGLSTMID WRNRDSYGKS
     ISSKNRAQLY RLRKWQRRIR VSNATERNLA FALSELDRMA SALGLPRTVR ETAAVVYRKA
     VDKNLIRGRS IEGVAAAALY AACRQCSVPR TLDEIEEVSR VSRKEIGRTY RFISRELALK
     LMPTSPIDYV PRFCSGLNLK GEVQSKSVEI LRQASEKELT SGRGPTGVAA AAIYIASILC
     GERRTQREVA DVAGVTEVTI RNRYKELAEE LDIEIIL