ID   TF2B_METMZ              Reviewed;         337 AA.
AC   P0CW14; Q977U3;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Transcription initiation factor IIB {ECO:0000255|HAMAP-Rule:MF_00383};
DE            Short=TFIIB {ECO:0000255|HAMAP-Rule:MF_00383};
GN   Name=tfb {ECO:0000255|HAMAP-Rule:MF_00383};
OS   Methanosarcina mazei (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S-6;
RX   PubMed=11397082; DOI=10.1006/jmbi.2001.4705;
RA   Thomsen J., de Biase A., Kaczanowski S., Macario A.J.L., Thomm M.,
RA   Zielenkiewicz P., MacColl R., Conway de Macario E.;
RT   "The basal transcription factors TBP and TFB from the mesophilic archaeon
RT   Methanosarcina mazeii; structure and conformational changes upon
RT   interaction with stress-gene promoters.";
RL   J. Mol. Biol. 309:589-603(2001).
CC   -!- FUNCTION: Stabilizes TBP binding to an archaeal box-A promoter. Also
CC       responsible for recruiting RNA polymerase II to the pre-initiation
CC       complex (DNA-TBP-TFIIB). {ECO:0000255|HAMAP-Rule:MF_00383}.
CC   -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00383}.
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DR   EMBL; AJ243755; CAC42919.1; -; Genomic_DNA.
DR   RefSeq; WP_011033712.1; NZ_JJRB01000066.1.
DR   AlphaFoldDB; P0CW14; -.
DR   SMR; P0CW14; -.
DR   IntAct; P0CW14; 1.
DR   MINT; P0CW14; -.
DR   GeneID; 44088592; -.
DR   GeneID; 66136747; -.
DR   OMA; DHDQRMK; -.
DR   OrthoDB; 35979at2157; -.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR   CDD; cd00043; CYCLIN; 2.
DR   HAMAP; MF_00383; TF2B_arch; 1.
DR   InterPro; IPR013763; Cyclin-like.
DR   InterPro; IPR036915; Cyclin-like_sf.
DR   InterPro; IPR000812; TFIIB.
DR   InterPro; IPR023484; TFIIB_arc.
DR   InterPro; IPR023486; TFIIB_CS.
DR   InterPro; IPR013150; TFIIB_cyclin.
DR   InterPro; IPR013137; Znf_TFIIB.
DR   PANTHER; PTHR11618; PTHR11618; 1.
DR   Pfam; PF08271; TF_Zn_Ribbon; 1.
DR   Pfam; PF00382; TFIIB; 2.
DR   PRINTS; PR00685; TIFACTORIIB.
DR   SMART; SM00385; CYCLIN; 2.
DR   SUPFAM; SSF47954; SSF47954; 2.
DR   PROSITE; PS00782; TFIIB; 2.
DR   PROSITE; PS51134; ZF_TFIIB; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Repeat; Transcription; Transcription regulation; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..337
FT                   /note="Transcription initiation factor IIB"
FT                   /id="PRO_0000119322"
FT   REPEAT          154..237
FT                   /note="1"
FT   REPEAT          248..329
FT                   /note="2"
FT   ZN_FING         37..68
FT                   /note="TFIIB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         41
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         44
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         60
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT   BINDING         63
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ   SEQUENCE   337 AA;  38290 MW;  39C58CA8D9ACD879 CRC64;
     MVEVERVRYS DTLEREKIRA MIKARKEKQK EQTVENEKAV CPECGSRNLV HDYERAELVC
     GDCGLVIDAD FVDEGPEWRA FDHDQRMKRS RVGAPMTYTI HDKGLSTMID WRNRDSYGKS
     ISSKNRAQLY RLRKWQRRIR VSNATERNLA FALSELDRMA SALGLPRTVR ETAAVVYRKA
     VDKNLIRGRS IEGVAAAALY AACRQCSVPR TLDEIEEVSR VSRKEIGRTY RFISRELALK
     LMPTSPIDYV PRFCSGLNLK GEVQSKSVEI LRQASEKELT SGRGPTGVAA AAIYIASILC
     GERRTQREVA DVAGVTEVTI RNRYKELAEE LDIEIIL