TF2B_PYRFU
ID TF2B_PYRFU Reviewed; 300 AA.
AC P61998; P29095; Q51731;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Transcription initiation factor IIB {ECO:0000255|HAMAP-Rule:MF_00383};
DE Short=TFIIB {ECO:0000255|HAMAP-Rule:MF_00383};
GN Name=tfb {ECO:0000255|HAMAP-Rule:MF_00383}; OrderedLocusNames=PF1377;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Zeng Q., Lewis L.M., Colangelo C.M., Dong J., Scott R.A.;
RT "A transcription factor (TFIIB) homolog from the hyperthermophilic archaeon
RT Pyrococcus furiosus binds Zn or Fe in an N-terminal Cys4 motif.";
RL J. Biol. Inorg. Chem. 1:162-168(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
RN [3]
RP STRUCTURE BY NMR OF 1-50.
RX PubMed=8564536; DOI=10.1038/nsb0296-122;
RA Zhu W., Zeng Q., Colangelo C.M., Lewis L.M., Summers M.F., Scott R.A.;
RT "The N-terminal domain of TFIIB from Pyrococcus furiosus forms a zinc
RT ribbon.";
RL Nat. Struct. Biol. 3:122-124(1996).
CC -!- FUNCTION: Stabilizes TBP binding to an archaeal box-A promoter. Also
CC responsible for recruiting RNA polymerase II to the pre-initiation
CC complex (DNA-TBP-TFIIB).
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000255|HAMAP-
CC Rule:MF_00383}.
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DR EMBL; U48391; AAC43724.1; -; Genomic_DNA.
DR EMBL; AE009950; AAL81501.1; -; Genomic_DNA.
DR PIR; T46883; T46883.
DR RefSeq; WP_011012524.1; NC_018092.1.
DR PDB; 1PFT; NMR; -; A=1-49.
DR PDBsum; 1PFT; -.
DR AlphaFoldDB; P61998; -.
DR SMR; P61998; -.
DR MINT; P61998; -.
DR STRING; 186497.PF1377; -.
DR EnsemblBacteria; AAL81501; AAL81501; PF1377.
DR GeneID; 41713185; -.
DR KEGG; pfu:PF1377; -.
DR PATRIC; fig|186497.12.peg.1440; -.
DR eggNOG; arCOG01981; Archaea.
DR HOGENOM; CLU_043736_0_1_2; -.
DR OMA; RTQKDFA; -.
DR OrthoDB; 35979at2157; -.
DR PhylomeDB; P61998; -.
DR EvolutionaryTrace; P61998; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR CDD; cd00043; CYCLIN; 2.
DR HAMAP; MF_00383; TF2B_arch; 1.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR023484; TFIIB_arc.
DR InterPro; IPR023486; TFIIB_CS.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS00782; TFIIB; 2.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..300
FT /note="Transcription initiation factor IIB"
FT /id="PRO_0000119327"
FT REPEAT 114..197
FT /note="1"
FT REPEAT 210..291
FT /note="2"
FT ZN_FING 2..34
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 7
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 10
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 26
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 29
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT TURN 8..10
FT /evidence="ECO:0007829|PDB:1PFT"
FT STRAND 15..18
FT /evidence="ECO:0007829|PDB:1PFT"
FT TURN 19..22
FT /evidence="ECO:0007829|PDB:1PFT"
FT STRAND 23..29
FT /evidence="ECO:0007829|PDB:1PFT"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:1PFT"
SQ SEQUENCE 300 AA; 34106 MW; 476D7CA32B2ED4C1 CRC64;
MNKQKVCPAC ESAELIYDPE RGEIVCAKCG YVIEENIIDM GPEWRAFDAS QRERRSRTGA
PESILLHDKG LSTEIGIDRS LSGLMREKMY RLRKWQSRLR VSDAAERNLA FALSELDRIT
AQLKLPRHVE EEAARLYREA VRKGLIRGRS IESVMAACVY AACRLLKVPR TLDEIADIAR
VDKKEIGRSY RFIARNLNLT PKKLFVKPTD YVNKFADELG LSEKVRRRAI EILDEAYKRG
LTSGKSPAGL VAAALYIASL LEGEKRTQRE VAEVARVTEV TVRNRYKELV EKLKIKVPIA
