TF2H1_DROME
ID TF2H1_DROME Reviewed; 585 AA.
AC Q960E8; A4UZH0; Q0E978;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=General transcription factor IIH subunit 1;
DE AltName: Full=TFIIH basal transcription factor complex subunit 1;
GN Name=Tfb1; ORFNames=CG8151;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:P32780}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which
CC is active in NER. The core complex associates with the 3-subunit CDK-
CC activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and
CC MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription. {ECO:0000250|UniProtKB:P32780}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFB1 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58253.2; -; Genomic_DNA.
DR EMBL; AE013599; AAM71001.1; -; Genomic_DNA.
DR EMBL; AY052097; AAK93521.1; -; mRNA.
DR RefSeq; NP_001246321.1; NM_001259392.2.
DR RefSeq; NP_610957.1; NM_137113.4.
DR RefSeq; NP_725382.1; NM_166048.1.
DR RefSeq; NP_725383.1; NM_166049.1.
DR AlphaFoldDB; Q960E8; -.
DR SMR; Q960E8; -.
DR BioGRID; 62348; 6.
DR DIP; DIP-21835N; -.
DR IntAct; Q960E8; 4.
DR STRING; 7227.FBpp0086676; -.
DR PaxDb; Q960E8; -.
DR PRIDE; Q960E8; -.
DR DNASU; 36598; -.
DR EnsemblMetazoa; FBtr0087548; FBpp0086676; FBgn0033929.
DR EnsemblMetazoa; FBtr0087549; FBpp0086677; FBgn0033929.
DR EnsemblMetazoa; FBtr0087550; FBpp0086678; FBgn0033929.
DR EnsemblMetazoa; FBtr0305616; FBpp0294063; FBgn0033929.
DR GeneID; 36598; -.
DR KEGG; dme:Dmel_CG8151; -.
DR UCSC; CG8151-RA; d. melanogaster.
DR CTD; 36598; -.
DR FlyBase; FBgn0033929; Tfb1.
DR VEuPathDB; VectorBase:FBgn0033929; -.
DR eggNOG; KOG2074; Eukaryota.
DR GeneTree; ENSGT00390000015066; -.
DR HOGENOM; CLU_037467_0_0_1; -.
DR InParanoid; Q960E8; -.
DR OMA; ENVPAKM; -.
DR OrthoDB; 946128at2759; -.
DR PhylomeDB; Q960E8; -.
DR Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-DME-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DME-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-DME-5696400; Dual Incision in GG-NER.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DME-6782135; Dual incision in TC-NER.
DR Reactome; R-DME-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DME-72086; mRNA Capping.
DR Reactome; R-DME-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-DME-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DME-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DME-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DME-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-DME-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR SignaLink; Q960E8; -.
DR BioGRID-ORCS; 36598; 1 hit in 1 CRISPR screen.
DR ChiTaRS; Tfb1; fly.
DR GenomeRNAi; 36598; -.
DR PRO; PR:Q960E8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033929; Expressed in eye disc (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q960E8; baseline and differential.
DR Genevisible; Q960E8; DM.
DR GO; GO:0005634; C:nucleus; ISS:FlyBase.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; ISS:FlyBase.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IDA:FlyBase.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; ISS:FlyBase.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0001111; P:promoter clearance from RNA polymerase II promoter; ISS:FlyBase.
DR GO; GO:0009411; P:response to UV; IMP:FlyBase.
DR GO; GO:0006360; P:transcription by RNA polymerase I; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:FlyBase.
DR GO; GO:0001113; P:transcription open complex formation at RNA polymerase II promoter; ISS:FlyBase.
DR Gene3D; 1.10.3970.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR005607; BSD_dom.
DR InterPro; IPR035925; BSD_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR027079; Tfb1/GTF2H1.
DR InterPro; IPR013876; TFIIH_BTF_p62_N.
DR PANTHER; PTHR12856; PTHR12856; 1.
DR Pfam; PF03909; BSD; 1.
DR Pfam; PF08567; PH_TFIIH; 1.
DR SMART; SM00751; BSD; 2.
DR SUPFAM; SSF140383; SSF140383; 2.
DR PROSITE; PS50858; BSD; 2.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..585
FT /note="General transcription factor IIH subunit 1"
FT /id="PRO_0000119247"
FT DOMAIN 104..159
FT /note="BSD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00036"
FT DOMAIN 180..232
FT /note="BSD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00036"
FT REGION 324..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..360
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 585 AA; 66339 MW; 629BE277BC6AF6C0 CRC64;
MTTSSEDVLL QMGEVRYKKG DGTLYVMNER VAWMAEHRDT VTVSHRYADI KTQKISPEGK
PKVQLQVVLH DGNTSTFHFV NRQGQAAMLA DRDKVKELLQ QLLPNFKRKV DKDLEDKNRI
LVENPNLLQL YKDLVITKVL TSDEFWATHA KDHALKKMGR SQEIGVSGAF LADIKPQTDG
CNGLKYNLTS DVIHCIFKTY PAVKRKHFEN VPAKMSEAEF WTKFFQSHYF HRDRLTAGTK
DIFTECGKID DQALKAAVQQ GAGDPLLDLK KFEDVPLEEG FGSVAGDRNV VNSGNIVHQN
MIKRFNQHSI MVLKTCANVT SAPSTMTNGT NNANGPVSQS AYTNGMNGKG QATATATKSS
SDQVDKDEPQ SKKQRLMEKI HYVDLGDPIL EGDDSANGEK AKSKHFELSK VERYLNGPVQ
NSMYDNHNDP MSLEEVQYKL VRNSESWLNR NVQRTFICSK AAVNALGELS PGGSMMRGFQ
EQSAGQLVPN DFQRELRHLY LSLSELLKHF WSCFPPTSEE LETKLQRMHE TLQRFKMAKL
VPFENRAMHE LSPLRSSLTQ HLNQLLRTAN SKFATWKERK LRNTR
