TF2H2_BOVIN
ID TF2H2_BOVIN Reviewed; 395 AA.
AC Q2TBV5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=General transcription factor IIH subunit 2;
DE AltName: Full=General transcription factor IIH polypeptide 2;
GN Name=GTF2H2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. The N-terminus of GTF2H2 interacts with
CC and regulates XPD whereas an intact C-terminus is required for a
CC successful escape of RNAP II form the promoter.
CC {ECO:0000250|UniProtKB:Q13888}.
CC -!- SUBUNIT: Component of the TFIID-containing RNA polymerase II pre-
CC initiation complex that is composed of TBP and at least GTF2A1, GTF2A2,
CC GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1,
CC ERCC2 and ERCC3. Component of the 7-subunit TFIIH core complex composed
CC of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5,
CC which is active in NER. The core complex associates with the 3-subunit
CC CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and
CC MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription. Interacts with XPB, XPD, GTF2H1 and GTF2H3.
CC {ECO:0000250|UniProtKB:Q13888}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13888}.
CC -!- SIMILARITY: Belongs to the GTF2H2 family. {ECO:0000305}.
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DR EMBL; BC109593; AAI09594.1; -; mRNA.
DR RefSeq; NP_001033619.1; NM_001038530.1.
DR AlphaFoldDB; Q2TBV5; -.
DR SMR; Q2TBV5; -.
DR STRING; 9913.ENSBTAP00000040137; -.
DR PaxDb; Q2TBV5; -.
DR Ensembl; ENSBTAT00000040362; ENSBTAP00000040137; ENSBTAG00000027983.
DR GeneID; 511829; -.
DR KEGG; bta:511829; -.
DR CTD; 2966; -.
DR VEuPathDB; HostDB:ENSBTAG00000027983; -.
DR eggNOG; KOG2807; Eukaryota.
DR GeneTree; ENSGT00490000043395; -.
DR InParanoid; Q2TBV5; -.
DR OrthoDB; 768809at2759; -.
DR Proteomes; UP000009136; Chromosome 20.
DR Bgee; ENSBTAG00000027983; Expressed in oocyte and 106 other tissues.
DR ExpressionAtlas; Q2TBV5; baseline and differential.
DR GO; GO:0000438; C:core TFIIH complex portion of holo TFIIH complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR CDD; cd01453; vWA_transcription_factor_IIH_type; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR046349; C1-like_sf.
DR InterPro; IPR007198; Ssl1-like.
DR InterPro; IPR004595; TFIIH_C1-like_dom.
DR InterPro; IPR012170; TFIIH_SSL1/p44.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF07975; C1_4; 1.
DR Pfam; PF04056; Ssl1; 1.
DR PIRSF; PIRSF015919; TFIIH_SSL1; 1.
DR SMART; SM01047; C1_4; 1.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR SUPFAM; SSF57889; SSF57889; 1.
DR TIGRFAMs; TIGR00622; ssl1; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..395
FT /note="General transcription factor IIH subunit 2"
FT /id="PRO_0000327565"
FT DOMAIN 60..236
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT ZN_FING 291..308
FT /note="C4-type"
FT MOD_RES 95
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:A0JN27"
SQ SEQUENCE 395 AA; 44512 MW; 485679E0E501390E CRC64;
MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH
LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP ISQIGIIVTK SKRAEKLTEL
SGNPRKHITS LKKAVDMTCH GEPSLYNSLS MAMQTLKHMP GHTSREVLII FSSLTTCDPS
NIYDLIKSLK AAKIRVSIIG LSAEVRVCTA LARETGGTYH VILDESHYKE LLTHHVSPPP
ASSNSECSLI RMGFPQHTIA SLSDQDAKPS FSMAHLDSNT EPGLTLGGYF CPQCRAKYCE
LPVECKICGL TLVSAPHLAR SYHHLFPLDA FQEIPLEEHN GERFCYACQG ELKDQHVYVC
SVCQNVFCVD CDVFVHDSLH CCPGCIHKIP VPSGI
