TF2H2_CAEEL
ID TF2H2_CAEEL Reviewed; 376 AA.
AC P34567;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 3.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=General transcription factor IIH subunit 2;
DE AltName: Full=TFIIH basal transcription factor complex subunit 2;
GN Name=gtf-2H2C {ECO:0000312|WormBase:T16H12.4};
GN ORFNames=T16H12.4 {ECO:0000312|WormBase:T16H12.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:Q13888}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of xpb-
CC 1, xpd-1, gtf-2H1, gtf-2H2C, gtf-2H3, Y73F8A.24 and gtf-2H5, which is
CC active in NER. The core complex associates with the 3-subunit CDK-
CC activating kinase (CAK) module composed of cyh-1, cdk-7 and mnat-1 to
CC form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.
CC {ECO:0000250|UniProtKB:Q13888}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GTF2H2 family. {ECO:0000305}.
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DR EMBL; BX284603; CAA83139.3; -; Genomic_DNA.
DR PIR; S42385; S42385.
DR RefSeq; NP_499239.1; NM_066838.3.
DR AlphaFoldDB; P34567; -.
DR SMR; P34567; -.
DR BioGRID; 41615; 1.
DR IntAct; P34567; 1.
DR STRING; 6239.T16H12.4.1; -.
DR EPD; P34567; -.
DR PaxDb; P34567; -.
DR PeptideAtlas; P34567; -.
DR EnsemblMetazoa; T16H12.4.1; T16H12.4.1; WBGene00011814.
DR GeneID; 176421; -.
DR KEGG; cel:CELE_T16H12.4; -.
DR UCSC; T16H12.4.1; c. elegans.
DR CTD; 176421; -.
DR WormBase; T16H12.4; CE29053; WBGene00011814; gtf-2H2C.
DR eggNOG; KOG2807; Eukaryota.
DR GeneTree; ENSGT00490000043395; -.
DR HOGENOM; CLU_028556_1_0_1; -.
DR InParanoid; P34567; -.
DR OMA; CMCHIEN; -.
DR OrthoDB; 768809at2759; -.
DR PhylomeDB; P34567; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-113418; Formation of the Early Elongation Complex.
DR Reactome; R-CEL-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-CEL-5696400; Dual Incision in GG-NER.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-CEL-6782135; Dual incision in TC-NER.
DR Reactome; R-CEL-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-CEL-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-CEL-72086; mRNA Capping.
DR Reactome; R-CEL-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-CEL-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-CEL-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-CEL-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-CEL-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-CEL-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR PRO; PR:P34567; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00011814; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IEA:InterPro.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd01453; vWA_transcription_factor_IIH_type; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR046349; C1-like_sf.
DR InterPro; IPR007198; Ssl1-like.
DR InterPro; IPR004595; TFIIH_C1-like_dom.
DR InterPro; IPR012170; TFIIH_SSL1/p44.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR000433; Znf_ZZ.
DR Pfam; PF07975; C1_4; 1.
DR Pfam; PF04056; Ssl1; 1.
DR PIRSF; PIRSF015919; TFIIH_SSL1; 1.
DR SMART; SM01047; C1_4; 1.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR SUPFAM; SSF57889; SSF57889; 1.
DR TIGRFAMs; TIGR00622; ssl1; 1.
DR PROSITE; PS50234; VWFA; 1.
DR PROSITE; PS01357; ZF_ZZ_1; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..376
FT /note="General transcription factor IIH subunit 2"
FT /id="PRO_0000119250"
FT DOMAIN 64..206
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT ZN_FING 286..303
FT /note="C4-type"
SQ SEQUENCE 376 AA; 41611 MW; 03E8CF77EA00766F CRC64;
MDDDEQKGYT WEAGYAEGLN INDVLVEDEG GSIEKSIAKY VADSKRKARL TKRPERIRLG
IMRHVMIVID CSRFMTSKAM PPSRFVVVMK ALQTFLDRFF EQNPIAQIGL ITCKDRKADR
LTMMTGNIRV LKESLNTLTE AFCGGDFSLQ NALQLACANL KGMPGHVSRE VVLVISALST
IDPGNIYSTI ETMKRMNIRC SAIGLSAEMF VCKEMAKATK GEYSVALDPD HLQLLFSKHT
LPPSSAKSSE CNAIHVGFPH HELITTRSFC VCHPDTKPIS SRGFICTQCG ARHCSIPAEC
PVCKLTLVAA PQLARAFRHL QPLSAFEQIE VTRGFCYACE TRLSGEGFRC GSCQLVFCLD
CDTLLHESLH VCPGCN
