TF2H3_MOUSE
ID TF2H3_MOUSE Reviewed; 309 AA.
AC Q8VD76;
DT 30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=General transcription factor IIH subunit 3;
DE AltName: Full=Basic transcription factor 2 34 kDa subunit;
DE Short=BTF2 p34;
DE AltName: Full=General transcription factor IIH polypeptide 3;
DE AltName: Full=TFIIH basal transcription factor complex p34 subunit;
GN Name=Gtf2h3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP INTERACTION WITH RARA.
RX PubMed=9230306; DOI=10.1016/s0092-8674(00)80317-7;
RA Rochette-Egly C., Adam S., Rossignol M., Egly J.-M., Chambon P.;
RT "Stimulation of RAR alpha activation function AF-1 through binding to the
RT general transcription factor TFIIH and phosphorylation by CDK7.";
RL Cell 90:97-107(1997).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:Q13889}.
CC -!- SUBUNIT: Part of a TFIID-containing RNA polymerase II pre-initiation
CC complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1,
CC GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2,
CC ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10,
CC TAF11, TAF12 and TAF13. Component of the 7-subunit TFIIH core complex
CC composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and
CC GTF2H5, which is active in NER. The core complex associates with the 3-
CC subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H,
CC CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription (By similarity). Interacts with RARA; the interaction
CC requires prior phosphorylation of RARA on 'Ser-369' which then enhances
CC interaction of RARA with CDK7 (PubMed:9230306).
CC {ECO:0000250|UniProtKB:Q13889, ECO:0000269|PubMed:9230306}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13889}.
CC -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000305}.
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DR EMBL; BC017515; AAH17515.1; -; mRNA.
DR CCDS; CCDS19680.1; -.
DR RefSeq; NP_852075.1; NM_181410.3.
DR AlphaFoldDB; Q8VD76; -.
DR SMR; Q8VD76; -.
DR BioGRID; 229071; 3.
DR IntAct; Q8VD76; 1.
DR STRING; 10090.ENSMUSP00000031333; -.
DR iPTMnet; Q8VD76; -.
DR PhosphoSitePlus; Q8VD76; -.
DR EPD; Q8VD76; -.
DR MaxQB; Q8VD76; -.
DR PaxDb; Q8VD76; -.
DR PRIDE; Q8VD76; -.
DR ProteomicsDB; 259012; -.
DR Antibodypedia; 19294; 130 antibodies from 25 providers.
DR DNASU; 209357; -.
DR Ensembl; ENSMUST00000031333; ENSMUSP00000031333; ENSMUSG00000029387.
DR GeneID; 209357; -.
DR KEGG; mmu:209357; -.
DR UCSC; uc012edo.1; mouse.
DR CTD; 2967; -.
DR MGI; MGI:1277143; Gtf2h3.
DR VEuPathDB; HostDB:ENSMUSG00000029387; -.
DR eggNOG; KOG2487; Eukaryota.
DR GeneTree; ENSGT00390000013143; -.
DR HOGENOM; CLU_040211_1_0_1; -.
DR InParanoid; Q8VD76; -.
DR OMA; CFCHRKV; -.
DR OrthoDB; 1220881at2759; -.
DR PhylomeDB; Q8VD76; -.
DR TreeFam; TF314336; -.
DR Reactome; R-MMU-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-MMU-113418; Formation of the Early Elongation Complex.
DR Reactome; R-MMU-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-MMU-5696400; Dual Incision in GG-NER.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-MMU-6782135; Dual incision in TC-NER.
DR Reactome; R-MMU-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-MMU-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-MMU-72086; mRNA Capping.
DR Reactome; R-MMU-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-MMU-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-MMU-73863; RNA Polymerase I Transcription Termination.
DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-MMU-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-MMU-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR BioGRID-ORCS; 209357; 27 hits in 111 CRISPR screens.
DR PRO; PR:Q8VD76; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8VD76; protein.
DR Bgee; ENSMUSG00000029387; Expressed in epiblast (generic) and 200 other tissues.
DR ExpressionAtlas; Q8VD76; baseline and differential.
DR Genevisible; Q8VD76; MM.
DR GO; GO:0000438; C:core TFIIH complex portion of holo TFIIH complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISO:MGI.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:UniProtKB.
DR GO; GO:0097550; C:transcription preinitiation complex; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:MGI.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12831; PTHR12831; 1.
DR Pfam; PF03850; Tfb4; 1.
DR TIGRFAMs; TIGR00627; tfb4; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..309
FT /note="General transcription factor IIH subunit 3"
FT /id="PRO_0000119252"
FT ZN_FING 269..286
FT /note="C4-type"
SQ SEQUENCE 309 AA; 34244 MW; 39CD6D1B8CD86E2D CRC64;
MAADEDELNL LVIIVDTNPI WWGKQALKES QFTLSKCMDA VMVLANSHLF MNRSNQLAVI
ASHIQESRLL YPGKNGGLGD FFGDPGNALP DCNPSGSKDG KYELLTVANE VIAEEIKDLM
TKSDIKGQHT ETLLAGSLAK ALCYIHRVNK AVKDNQEMKS RILVIKAAED SALQYMNFMN
VIFAAQKQNI LIDACVLDSD SGLLQQACDI TGGLYLKVPQ MPSLLQYLLW VFLPDQDQRS
QLILPPPIHV DYRAACFCHR SLIEIGYVCS VCLSIFCNFS PICTTCETAF KISLPPVLKA
KKKKQKVSL
