TF2H3_RAT
ID TF2H3_RAT Reviewed; 309 AA.
AC Q561R7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=General transcription factor IIH subunit 3;
DE AltName: Full=General transcription factor IIH polypeptide 3;
GN Name=Gtf2h3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:Q13889}.
CC -!- SUBUNIT: Part of a TFIID-containing RNA polymerase II pre-initiation
CC complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1,
CC GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2,
CC ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10,
CC TAF11, TAF12 and TAF13. Component of the 7-subunit TFIIH core complex
CC composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and
CC GTF2H5, which is active in NER. The core complex associates with the 3-
CC subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H,
CC CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription (By similarity). Interacts with RARA; the interaction
CC requires prior phosphorylation of RARA on 'Ser-369' which then enhances
CC interaction of RARA with CDK7 (By similarity).
CC {ECO:0000250|UniProtKB:Q13889, ECO:0000250|UniProtKB:Q8VD76}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13889}.
CC -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000305}.
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DR EMBL; BC093380; AAH93380.1; -; mRNA.
DR RefSeq; NP_001019407.1; NM_001024236.1.
DR AlphaFoldDB; Q561R7; -.
DR SMR; Q561R7; -.
DR STRING; 10116.ENSRNOP00000001368; -.
DR PaxDb; Q561R7; -.
DR Ensembl; ENSRNOT00000001368; ENSRNOP00000001368; ENSRNOG00000001035.
DR GeneID; 288651; -.
DR KEGG; rno:288651; -.
DR UCSC; RGD:1305494; rat.
DR CTD; 2967; -.
DR RGD; 1305494; Gtf2h3.
DR eggNOG; KOG2487; Eukaryota.
DR GeneTree; ENSGT00390000013143; -.
DR HOGENOM; CLU_040211_1_0_1; -.
DR InParanoid; Q561R7; -.
DR OMA; CFCHRKV; -.
DR OrthoDB; 1220881at2759; -.
DR PhylomeDB; Q561R7; -.
DR TreeFam; TF314336; -.
DR Reactome; R-RNO-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-RNO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-RNO-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-RNO-5696400; Dual Incision in GG-NER.
DR Reactome; R-RNO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-RNO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-RNO-6782135; Dual incision in TC-NER.
DR Reactome; R-RNO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-RNO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-RNO-72086; mRNA Capping.
DR Reactome; R-RNO-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-RNO-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-RNO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-RNO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-RNO-73863; RNA Polymerase I Transcription Termination.
DR Reactome; R-RNO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-RNO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-RNO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-RNO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR PRO; PR:Q561R7; -.
DR Proteomes; UP000002494; Chromosome 12.
DR Bgee; ENSRNOG00000001035; Expressed in thymus and 20 other tissues.
DR Genevisible; Q561R7; RN.
DR GO; GO:0000438; C:core TFIIH complex portion of holo TFIIH complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISO:RGD.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:UniProtKB.
DR GO; GO:0097550; C:transcription preinitiation complex; ISO:RGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:RGD.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:RGD.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12831; PTHR12831; 1.
DR Pfam; PF03850; Tfb4; 1.
DR TIGRFAMs; TIGR00627; tfb4; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..309
FT /note="General transcription factor IIH subunit 3"
FT /id="PRO_0000329322"
FT ZN_FING 269..286
FT /note="C4-type"
SQ SEQUENCE 309 AA; 34278 MW; 2D985D1FD32599B3 CRC64;
MAADEDELNL LVIIVDTNPI WWGKQALKES QFTLSKCMDA VMVLANAHLF MNRSNQLAVI
ASHIQESRFL YPGKNGRLGD FFGDPGNALP DCNPSGSKDG KYELLTAANE VIAEEIKDLM
TKSDIKGQHT ETLLAGSLAK ALCYIHRASK AVKDNQEMKS RILVIKAAED SALQYMNFMN
VIFAAQKQNI LIDACVLDSD SGLLQQACDI TGGLYLKVPQ MPSLLQYLLW VFLPDQDQRS
QLILPPPIHV DYRAACFCHR SLIEIGYVCS VCLSIFCNFS PICTTCETAF KISLPPVLKA
KKKKQKVSL
